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Modified Organism
Cantaloupe A (delayed ripening)
Record information and status
Record ID
15388
Status
Published
Date of creation
2006-07-13 13:31 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2012-07-25 19:10 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-07-25 19:10 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Cantaloupe A (delayed ripening)
Transformation event
Cantaloupe A
Developer(s)
Agritope, Inc
Url:Agritope Homepage
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Cucumis melo - Melon, Melons
Characteristics of the transformation process
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Introduced or modified genetic elements
Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
Neomycin Phosphotransferase II - Escherichia coli - ECOLX
Resistance to antibiotics - Kanamycin
S-adenosylmethionine hydrolase gene - Bacteriophage T3 - Phage T3, T3
Changes in physiology and/or production - Ripening
Notes regarding the genetic elements introduced or modified in this LMO
Canteloupe with delayed ripening due to expression of the SAMase gene from Escherichia coli bacteriophage T3
LMO characteristics
Modified traits
Additional Information
Additional Information
The A and B lines of cantaloupe (Cucumis melo) were developed through a specific genetic modification to express a reduced accumulation of S-adenosylmethionine (SAM) and consequently the trait of delayed ripening. This was accomplished by the introduction of a bacteriophage encoded enzyme, S-adenosylmethionine hydrolase, capable of degrading and thus reducing SAM. The conversion of SAM to 1-aminocyclopropane-1-carboxylic acid (ACC) is the first step in ethylene biosynthesis and the lack of sufficient pools of SAM results in significantly reduced synthesis of this phytohormone, which is known to play a key role in fruit ripening.

These lines were created by Agrobacterium-mediated transformation in which the transfer-DNA (T-DNA) contained the S-adenosylmethionine hydrolase encoding SAMase gene from Escherichia coli bacteriophage T3. The constitutive expression of the SAMase gene was controlled by inclusion of regulatory DNA sequences from A. tumefaciens. In addition, the T-DNA contained sequences encoding the enzyme neomycin phosphotransferase II (NPTII) from the Tn5 transposon of Escherichia coli, strain K12, under the control of the nos promoter from A. tumefaciens. The expression of NPTII activity was used as a selectable trait to screen transformed plants for the presence of the SAMase gene.

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