| | english | español | français |
Go to record ID

  Home|Finding Information|Record details   Printer-friendly version

Modified Organism
New Leaf™ Plus Russet Burbank potato
Record information and status
Record ID
15391
Status
Published
Date of creation
2006-07-13 14:01 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-04-25 16:15 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-04-25 16:15 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
New Leaf™ Plus Russet Burbank potato
Transformation event
RBMT22-238
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
Potatoes with insect-resistance and resistance to potato leafroll virus through inclusion of the cry3A gene from Bacillus thuringiensis which confers resistance to coleopteran pests, and DNA sequences corresponding to potato leafroll virus (PLRV) helicase and replicase domains which confers resistance to PLRV.  The epsps gene confers tolerance to the herbicide glyphosate. 
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Related LMOs
NMK-89896-6 - New Leaf™ Plus Russet Burbank potato
Resistance to diseases and pests - Insects - Coleoptera (beetles), Viruses - Potato leaf roll virus (PLRV) Resistance to herbicides - Glyphosate
New Leaf™ Plus Russet Burbank potato
Resistance to diseases and pests - Insects - Coleoptera (beetles), Viruses - Potato leaf roll virus (PLRV) Resistance to herbicides - Glyphosate
New Leaf™ Plus Russet Burbank potato
Resistance to diseases and pests - Insects - Coleoptera (beetles), Viruses - Potato leaf roll virus (PLRV) Resistance to herbicides - Glyphosate
Characteristics of the transformation process
Vector
PV-STMT22
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
rbcS Promoter
1.70 Kb
 
 
Cry3A
1.80 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
 
FMV 35S promoter
0.57 Kb
 
 
HSP17.9 Leader Sequence
0.08 Kb
 
 
PLRV Replicase gene
3.40 Kb
 
 
rbcS-E9 gene terminator
0.36 Kb
 
 
FMV 35S promoter
0.57 Kb
 
 
Chloroplast transit peptide 2
0.23 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.40 Kb
 
 
rbcS-E9 gene terminator
0.63 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The coding sequence of the Cry3A gene was modified to plant preferred codons. This resulted in changes to 399 of 1791 nucleotides but there were no changes to the resulting amino acid sequence.

PCR analysis indicated that the transformation cassette was inserted into the host genome in the same orientation as in the vector. Additionally no fragments outside the left and right border were detected.
LMO characteristics
Modified traits
Common use(s)
  • Food
Additional Information
Additional Information
Resistance to attack by CPB was accomplished by introducing the cry3A gene from Bacillus thuringiensis subsp. tenebrionis, which encodes an insecticidal crystalline Cry3A delta-endotoxin protein. The insecticidal activity of Cry3A protein is due to its selective binding to specific sites localized on the brush border midgut epithelium of susceptible insect species. Following binding, cation-specific pores are formed that disrupt midgut ion flow and thereby cause gut paralysis, ultimately leading to bacterial sepsis and death. Delta-endotoxins, such as the Cry3A protein expressed in CPB resistant potato lines, exhibit highly selective insecticidal activity against a narrow range of coleopteran insects such as CPB, elm leaf beetle and yellow mealworm. Their specificity of action is directly attributable to the presence of specific receptors in the target insects.

Introducing DNA sequences corresponding to the ORF-1 and ORF-2 regions from PLRV conferred resistance to PLRV infection. These two ORFs encode the putative viral helicase and replicase domains that are required for viral RNA synthesis. The introduced viral sequences do not result in the formation of any infectious particles, nor does their expression result in any disease pathology. These transgenic potato cultivars exhibit the trait of resistance to infection and subsequent disease caused by PLRV through an incompletely understood process that has been termed "replicase-mediated resistance", which may involve silencing of viral gene translation.

The line was also transformed to express resistance to glyphosate, the active ingredient in the herbicide Roundup®, through incorporation of a bacterial gene encoding a glyphosate-tolerant form of the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS).
Other relevant website address or attached documents

Records referencing this document (4)
IDDescription
4record(s) found
Country's Decision or any other Communication1 record
Modified Organism3 records