Information on the inserted DNA sequences:
The cp4epsps coding sequence cassette consists of the figwort
mosaic virus (FMV) promoter, a chloroplast targeting sequence from
Arabidosis thaliana, a CP4 EPSPS coding region from Agrobacterium
sp. strain CP4. The CP4 EPSPS protein is highly resistant to
inhibition by glyphosate, the active ingredient in Roundup
herbicide.The uidA coding region for the B-D-glucuronidase (GUS)
protein from E. coli and the 3' non-translated region from pea
which directs polyadenylation. This gene serves as a selectable
marker gene during the plant transformation process.The gox coding
sequence cassette consists of the figworth mosaic virus (FMV)
promoter, a chloroplast targeting sequence from Arabidosis thaliana
promoter coding region from Ochrobactrum anthropi and the 3'
non-translated region of the nopaline synthase gene, which
directs polyadenylation. When expressed, the function of the
glyphosate oxidase (GOX) enzyme is to metabolize glyphosate
(N-phosphonomethylglycine), the active ingredient in Roundup
herbicide to an inactive form.
Vector information
The plasmid vector PV-BVGT03 contains well characterized DNA
segments required for selection and replication of the plasmid in
the bacteria as well as a right border for initiating the region of
T-DNA, into the plant genomic DNA. The plasmids are composed of
several genetic elements: cp4 epsps coding sequence from
Agrobacterium tumefaciens, uid sequence (GUS) from Escherichi coli,
gox sequence from Ochrobactrum anthropi, and nptII coding sequence
from Tn5. In addition, the plasmid contains a bacterial selectable
marker coding sequence, aad as well as origins of replication
(ori-V and ori-322) necessary for replication and maintenance of
the plasmid PV-BVGT03 in bacteria.
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