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Modified Organism
SY-GTSB77-7 - InVigor™ Sugar Beet
Record information and status
Record ID
15409
Status
Published
Date of creation
2006-07-13 15:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2015-08-27 15:48 UTC (dina.abdelhakim@cbd.int)
Date of publication
2015-08-27 15:48 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
InVigor™ Sugar Beet
Transformation event
GTSB77
Unique identifier
SY-GTSB77-7
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
Sugar beet line 77 was produced to exhibit tolerance to the herbicide glyphosate (Roundup) by incorporating the 5-enolpyruvylshikimate-3-phosphate synthase (cp4 epsps) gene from Agrobacterium sp. strain CP4 and a glyphosate oxidoreductase gene (gox) from Ochrobactrum anthropi.

NOTE: This LMO was formerly referred to with the UID SY-GTSB77-8.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Beta vulgaris - Common beet, Sugarbeet, BETMA
Characteristics of the transformation process
Vector
PV-BVGT03
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
FMV 35S promoter
0.67 Kb
 
 
Chloroplast transit peptide 2
0.31 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.36 Kb
 
 
rbcS-E9 gene terminator
0.63 Kb
 
 
CaMV Enhanced 35S promoter
0.62 Kb
 
 
Beta-Glucuronidase coding sequence
1.81 Kb
 
 
rbcS-E9 gene terminator
0.63 Kb
 
 
FMV 35S promoter
0.67 Kb
 
 
rbcS Transit Peptide
0.17 Kb
 
 
Glyphosate oxidoreductase gene
1.30 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Information on the inserted DNA sequences:
The codon optimised cp4epsps coding sequence cassette consists of the figwort mosaic virus (FMV) promoter, a chloroplast targeting sequence from Arabidosis thaliana, a CP4 EPSPS coding region from Agrobacterium sp. strain CP4. The CP4 EPSPS protein is highly resistant to inhibition by glyphosate, the active ingredient in Roundup herbicide.The uidA coding region for the B-D-glucuronidase (GUS) protein from E. coli and the 3' non-translated region from pea which directs polyadenylation. This gene serves as a selectable marker gene during the plant transformation process.The gox coding sequence cassette consists of the figworth mosaic virus (FMV) promoter, a chloroplast targeting sequence from Arabidosis thaliana promoter coding region from Ochrobactrum anthropi and the 3' non-translated region of  the nopaline synthase gene, which directs polyadenylation. When expressed, the function of the glyphosate oxidase (GOX) enzyme is to metabolize glyphosate (N-phosphonomethylglycine), the active ingredient in Roundup herbicide to an inactive form.

Vector information
The plasmid vector PV-BVGT03 contains well characterized DNA segments required for selection and replication of the plasmid in the bacteria as well as a right border for initiating the region of T-DNA, into the plant genomic DNA. The plasmids are composed of several genetic elements: cp4 epsps coding sequence from Agrobacterium tumefaciens, uid sequence (GUS) from Escherichi coli, gox sequence from Ochrobactrum anthropi, and nptII coding sequence from Tn5. The nptII gene was not transferred into the sugar beet genome because of the truncation of the insertion event within the gox gene. In addition, the plasmid contains a bacterial selectable marker coding sequence, aad as well as origins of replication (ori-V and ori-322) necessary for replication and maintenance of the plasmid PV-BVGT03 in bacteria.

The truncated nptII construct was as follows: CaMV 35S promoter >> nptII gene >> nos terminator
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Food
  • Feed
Detection method(s)
Additional information
Southern blot analysis indicates that a single copy of the epsps gene integrated into the host genome in addition to single copies of the gus and gox expression cassettes. However nuleotide sequencing analyses indicated that the gox coding sequence did not fully integrate into the host genome and is thus present in a truncated form. Protein expression analysis further indicated that the gox is expressed in a truncated, chimeric and incative form.
Additional Information
Other relevant website address or attached documents

Records referencing this document (8)
IDDescription
8record(s) found
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Organization1 record
Risk Assessment3 records