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Modified Organism
Insect Resistant Maize MON801
Record information and status
Record ID
15410
Status
Published
Date of creation
2006-07-13 15:51 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-05-24 16:37 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-05-24 16:37 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Insect Resistant Maize MON801
Transformation event
MON801
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
Insect-resistant maize produced by inserting the full form of the cry1Ab gene from Bacillus thuringiensis subsp. kurstaki HD-1. The genetic modification affords resistance to attack by the European corn borer (ECB), Ostrinia nubilalis.  The nptII gene confers resistance to the antibiotic kanamycin and is used as a genetic marker.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Related LMOs
PH-MON8Ø9-2 - Insect-resistant maize MON809
Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glyphosate
MON-ØØ81Ø-6 - YieldGard™ maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Show detection method(s)
Characteristics of the transformation process
Vector
PV-ZMBK07 and PV-ZMGT10
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
CaMV Enhanced 35S promoter
0.62 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Cry1Ab
3.50 Kb
 
 
Nopaline Synthase Gene Terminator
0.24 Kb
 
 
CaMV Enhanced 35S promoter
0.62 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Chloroplast transit peptide 2
0.31 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.40 Kb
 
 
Nopaline Synthase Gene Terminator
0.24 Kb
 
 
CaMV Enhanced 35S promoter
0.62 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
rbcS Transit Peptide
0.26 Kb
 
 
Glyphosate oxidoreductase gene
1.30 Kb
 
 
Nopaline Synthase Gene Terminator
0.24 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The PV-ZMBK07 plasmid contained the coding sequence of a synthetic version of the cry1Ab gene from Bacillus thuringiensis, which was modified to enhance the expression of the Cry1Ab protein in plants, however the resulting amino acid sequence is identical to the native protein.

PV-ZMGT10 plasmid contained the genes cp4 epsps and gox as well as the antibiotic resistance marker gene nptII.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Additional Information
The transgenic maize line MON801 was genetically engineered to resist ECB by producing its own insecticide. This line was developed by introducing the cry1Ab gene, isolated from the common soil bacterium Bacillus thuringiensis (Bt), into the maize cultivar Hi-II by particle acceleration (biolistic) transformation. The cry1Ab gene produces the insect control protein Cry1Ab, a delta-endotoxin.

The insecticidally active portion of the Cry1Ab protein produced by the Bt maize is identical to that found in nature and in commercial Bt spray formulations. Cry proteins, of which Cry1Ab is only one, act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow causing gut paralysis and eventual death due to bacterial sepsis. Cry1Ab is insecticidal only when eaten by the larvae of lepidopteran insects (moths and butterflies), and its specificity of action is directly attributable to the presence of specific binding sites in the target insects. There are no binding sites for delta-endotoxins of B. thuringiensis on the surface of mammalian intestinal cells, therefore, livestock animals and humans are not susceptible to these proteins.

The nptII gene was isolated from transposon Tn5 in Klebsiella pneumonia. The nptII protein, neomycin phosphotransferase II, confers resistance to some aminoglycoside antibiotics including neomycin and kanamycin, and was used as a selectable marker for transformed plant cells.
Other relevant website address or attached documents

Records referencing this document (4)
IDDescription
4record(s) found
Country's Decision or any other Communication1 record
Modified Organism2 records
Organization1 record