| | english | español | français |
Go to record ID

  Home|Finding Information|Record details   Printer-friendly version

Modified Organism
MON-89788-1 - Roundup Ready2Yield™ soybean
Record information and status
Record ID
40284
Status
Published
Date of creation
2007-08-10 17:41 UTC (giovanni.ferraiolo@cbd.int)
Date of last update
2014-01-22 20:48 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-01-22 20:49 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Roundup Ready2Yield™ soybean
Transformation event
MON89788
Unique identifier
MON-89788-1
Developer(s)
Description
MON89788 is a second-generation glyphosate-tolerant soybean product, was developed by introduction of the cp4 epsps gene cassette consisting of chimeric promoter sequence derived from Arabidopsis thaliana Tsf1 gene promoter and enhancer sequences and from the 35S of the Figwort Mosaic Virus. In addition, the transformation was based on a new technique of Agrobacterium-mediated gene delivery to soybean meristem, where cells were induced directly to form shoots and give rise to transgenic plants
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Glycine max - Soybean, Soya bean, Soya, SOYBN
Point of collection or acquisition of the recipient organism
Variety: A3244
Related LMOs
MON-Ø4Ø32-6 - Roundup Ready™ soybean
Resistance to herbicides - Glyphosate
Show detection method(s)
Characteristics of the transformation process
Vector
PV-GMGOX20
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
FMV 35S Enhancer
1.04 Kb
 
 
Elongation factor EF-1alpha promoter
0.00 Kb
 
 
Elongation factor EF-1alpha Leader
0.05 Kb
 
 
Elongation factor EF-1alpha Intron 1
0.62 Kb
 
 
Chloroplast transit peptide 2
0.23 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.37 Kb
 
 
rbcS-E9 gene terminator
0.64 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Information on the inserted DNA sequences:
The DNA inserted into the soybean genome contains:
- Codon optimized coding sequence of the aroA (epsps) gene from the Agrobacterium sp. strain CP4 encoding the CP4 EPSPS protein.
- a chimeric transcriptional promoter (P-FMV/Tsf1) consisting of chimeric sequence derived from Arabidopsis thaliana Tsf1 gene promoter and enhancer sequences from  the 35S of the Figwort Mosaic Virus. Located between the promoter and the cp4epsps coding sequence are the non-translated leader sequence (L-Tsf1) and the I-Tsf1 non translated intron;
- a chloroplast transit peptide sequence (TS-CTP2), derived from the Arabidopsis thaliana epsps gene and placed before the cp4 epsps gene encoding sequence in the gene insert;
- a polyadenylation sequence from RbcS2 gene (T-E9), derived from Pisum sativum containing the 3' non translated region of the pea ribulose-1,5 biphosphate carboxylase small sub unit E9.

Vector information
The PV-GMGOX20 vector is approximately 9.7 kb and contains a cp4 epsps gene expression cassette delineated by left and right border regions. The T-DNA (transferred DNA) that is incorporated into the soybean genome is approximately 4.3 kb, and the DNA backbone region that is not incorporated into the soybean genome is approximately 5.4kb.

Southern blot analyses indicate that MON 89788 contains a single intact cp4 epsps expression cassette integrated at a single locus within the soybean genome. DNA sequencing analyses of the MON 89788 insert confirm the expected coding region of the cp4 epsps gene cassette, is identical to that transformed in the T-DNA cassette. No backbone sequences from the transformation plasmid were detected and no partial genetic elements, linked or unlinked to the inserted expression cassette were detected.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Additional Information
The soybean line MON98788 was developed to allow for the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option for soybean. This genetically engineered soybean variety contains a glyphosate tolerant form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) isolated from the common soil bacterium, Agrobacterium tumefaciens strain CP4 (CP4 EPSPS).

The EPSPS enzyme is part of the shikimate pathway that is involved in the production of aromatic amino acids and other aromatic compounds in plants. When conventional plants are treated with glyphosate, the plants cannot produce the aromatic amino acids needed to survive. This enzyme is present in all plants, bacteria, fungi, but not in animals, which do not synthesize their own aromatic amino acids. Because the aromatic amino acid biosynthetic pathway is not present in mammalian, avian or aquatic life forms, glyphosate has little if any toxicity for these organisms. The EPSPS enzyme is normally present in food derived from plant and microbial sources.

Records referencing this document (55)
IDDescription
55record(s) found
Country's Decision or any other Communication16 records
Modified Organism6 records
Organization18 records
Risk Assessment15 records