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Modified Organism
SYN-IR6Ø4-5 x MON-ØØØ21-9 - Agrisure™ RW Rootworm-Protected Roundup Ready™ maize
Record information and status
Record ID
43630
Status
Published
Date of creation
2008-01-10 13:06 UTC (manoela.miranda@cbd.int)
Date of last update
2015-10-20 06:49 UTC (manoela.miranda@cbd.int)
Date of publication
2015-10-20 06:49 UTC (manoela.miranda@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Agrisure™ RW Rootworm-Protected Roundup Ready™ maize
Transformation event
MIR604 x GA21
Unique identifier
SYN-IR6Ø4-5 x MON-ØØØ21-9
Developer(s)
Syngenta Crop Protection AG
Schwarzwaldallee 215
Basel
Switzerland, CH 4058
Phone:+41 61 323-1111
Url:Syngenta
Description
SYN-IR6Ø4-5×MON-ØØØ21-9 has stacked insect resistant and herbicide tolerant maize produced by conventional cross breeding of two genetically engineered parental varieties (MIR604 and GA21).

The parental MIR604 maize variety (OECD unique identifier SYN-IR6Ø4-5) was genetically modified by introducing the cry3A gene from Bacillus thuringiensis ssp. kumamotoensis, which codes for a  Bt-toxin (Cry3A) and confers resistance to western corn rootworm (Diabrotica virgifera virgifera), northern corn rootworm (Diabrotica longicornis barberi) and other related coleopteran species. Expression of the pmi gene in MIR604 from the bacterium Escherichia coli allows this line to use mannose as a carbon source through production of the PMI protein, and is used as a selectable marker.

The parental GA21 line (OECD unique identifier MON-ØØØ21-9) was created by modifying the endogenous maize epsps gene through site-directed mutagenesis, such that its encoded enzyme was insensitive to inactivation by glyphosate, and inserting it into the inbred AT maize variety. The modified maize GA21 line permits farmers to use glyphosate-containing herbicides for weed control in the cultivation of maize.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
SYN-IR6Ø4-5 - Agrisure™ RW Rootworm-Protected maize
Mannose tolerance Resistance to diseases and pests - Insects - Coleoptera (beetles) - Western corn rootworm (Diabrotica virgifera) Selectable marker genes and reporter genes
Show detection method(s)
MON-ØØØ21-9 - Roundup Ready™ maize
Resistance to herbicides - Glyphosate
Show detection method(s)
Characteristics of the transformation process
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
Rice actin 1 gene promoter
1.37 Kb
 
 
Rice actin 1, intron
0.00 Kb
 
 
Optimized Transit Peptide
0.37 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase
1.34 Kb
 
 
Nopaline Synthase Gene Terminator
0.24 Kb
 
 
Metallothionein-like gene promoter
0.00 Kb
 
 
mCry3A
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
Ubiquitin gene promoter
0.00 Kb
 
 
Ubiquitin Intron 1
0.00 Kb
 
 
Phosphomannose Isomerase gene
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Inserted genes (MIR604): 
1) cry3A-gene from Bacillus thuringiensis ssp. kumamotoensis. This gene codes for a Bt-toxin, which confers resistance to western corn rootworm (Diabrotica virgifera virgifera), northern corn rootworm (Diabrotica longicornis barberi) and other related coleopteran species.

2) Marker gene pmi-gene from the bacterium Escherichia coli. The LMO plant harboring this gene expresses a marker protein, PMI, that allows the plant to utilise mannose as a carbon source, acting as a selectable marker.

Inserted gene (GA21): 
m epsps. This is a mutated form of the epsps gene derived from maize (Zea mays). The cp4 epsps gene encodes for a version of EPSPS that is highly tolerant to inhibition by glyphosate.

LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
  • Mannose tolerance
Common use(s)
  • Food
Additional Information
Additional Information
This plant is a product of traditional plant breeding, and therefore is not automatically subject to regulation in all jurisdictions as are transgenic plants resulting from recombinant DNA technologies. Certain jurisdictions may request notification in advance of the release of a stacked hybrid, or may request information to conduct an environmental and food safety assessment.
Other relevant website address or attached documents

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Organization4 records
Risk Assessment9 records