GlyTol™ Cotton GHB614

GlyTol cotton event GHB614 contains a stably integrated gene 2mepsps. The 2mepsps gene was generated by introducing mutations into the wild-type epsps (wt epsps) gene from maize, leading to a double mutant EPSPS protein with two amino acid substitutions (2mEPSPS). This modification confers the protein a decreased binding affinity for glyphosate, allowing it to maintain sufficient enzymatic activity in the presence of the herbicide. Therefore, the plants bearing this gene are tolerant to glyphosate herbicides.

Variety: Coker 312

pTEM2

Southern blot analysis indicated that a single insertion of the complete T-DNA as constructed occurred in the GHB614 line. There was no indication that any portion of the vector backbone also integrated into the GHB614 line.