GlyTol cotton event GHB614 contains a stably integrated gene
2mepsps. The 2mepsps gene was generated by introducing mutations
into the wild-type epsps (wt epsps) gene from maize, leading to a
double mutant EPSPS protein with two amino acid substitutions
(2mEPSPS). This modification confers the protein a decreased
binding affinity for glyphosate, allowing it to maintain sufficient
enzymatic activity in the presence of the herbicide. Therefore, the
plants bearing this gene are tolerant to glyphosate herbicides.