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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect-resistant cotton
EN
COT67B
Yes
SYN-IR67B-1
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Organization:Syngenta ()Phone:Fax:Email:Website: http://www.syngentaseeds.com,
Cotton resistant to lepidopteran pests through introduction of the cry1Ab gene which codes for the Cry1Ab insecticidal protein that targets lepidopteran insect species.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12080-6 Organism Gossypium hirsutum (Cotton)Crops
Cultivar: Coker 312
EN
pNOV4641 and pNOV1914
EN
- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-101416-7 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-104517-2 Actin 2 promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoter
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-101874-2 Ubiquitin gene 3 promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoter
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BCH-GENE-SCBD-14991-8 Hygromycin B phosphotransferase gene | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Hygromycin),Selectable marker genes and reporter genes
The conventional cotton line Coker 312 was transformed by an Agrobacterium-mediated transformation using two binary vectors, pNOV4641 and pNOV1914. Once transformants were identified, Syngenta used traditional breeding techniques to select plants containing flcry1Ab but not the aph4 gene from pNOV1914 or elements from its plasmid backbone.
Genetic Elements in Plasmid pNOV4641:
Contains a synthetic full-length cry1Ab gene – designated flcry1Ab – originally derived from B. thuringiensis, subspecies kurstaki HD-1, which expresses the insecticidal FLCry1Ab protein
Genetic Elements in Plasmid pNOV1914:
Contains the aph4 gene derived from Escherichia coli, which expresses the enzyme hygromycin-B phosphotransferase for hygromycin resistance. The aph4 gene is used as a selectable marker for the transformation process.
Information on the inserted DNA sequences (in Spanish):
El gen insertado en el algodón COT67B, cry1Ab confiere resistencia a lepidopteros al codificar la proteina Cry1Ab, el gen proviene de Bacillus thuringiensis subsp. kurstaki.
Se utilizó el sistema de transformación de A. tumefaciens. Los genes insertados en COT67B, junto con los componentes necesarios para su regulación en planta son: Gen cry1Ab que codifica la proteína Cry1Ab; Promotor Act2 de Arabidosis thaliana que confiere la expression de gen cry1Ab; nos 3’ (terminador del gen principal); gen aph4 de E. coli (Marcador de selección) que fue descartado y no se encuentra en las plantas derivadas de COT67B; gen ubi3 de Arabidosis thaliana (promotor del gen marcador); nos 3’ (terminador del gen marcador).
EN
Genetic Elements in Plasmid pNOV4641:
Contains a synthetic full-length cry1Ab gene – designated flcry1Ab – originally derived from B. thuringiensis, subspecies kurstaki HD-1, which expresses the insecticidal FLCry1Ab protein
Genetic Elements in Plasmid pNOV1914:
Contains the aph4 gene derived from Escherichia coli, which expresses the enzyme hygromycin-B phosphotransferase for hygromycin resistance. The aph4 gene is used as a selectable marker for the transformation process.
Information on the inserted DNA sequences (in Spanish):
El gen insertado en el algodón COT67B, cry1Ab confiere resistencia a lepidopteros al codificar la proteina Cry1Ab, el gen proviene de Bacillus thuringiensis subsp. kurstaki.
Se utilizó el sistema de transformación de A. tumefaciens. Los genes insertados en COT67B, junto con los componentes necesarios para su regulación en planta son: Gen cry1Ab que codifica la proteína Cry1Ab; Promotor Act2 de Arabidosis thaliana que confiere la expression de gen cry1Ab; nos 3’ (terminador del gen principal); gen aph4 de E. coli (Marcador de selección) que fue descartado y no se encuentra en las plantas derivadas de COT67B; gen ubi3 de Arabidosis thaliana (promotor del gen marcador); nos 3’ (terminador del gen marcador).
EN
- Food
- Feed
- Fiber/textile
COT67B cotton was generated through the transfer of the full length cry1Ab gene (flcry1Ab) to the conventional cotton line Coker 312. The flcry1Ab gene, derived from B. thuringiensis subspecies kurstaki HD-1, has been modified to restore a 26 amino acid motif in the C-terminus of the protein which had been lost during a natural recombination event which generated the wild type cry1Ab gene. The flcry1Ab gene encodes FLCry1Ab, a full-length Cry protein consisting of 1181 amino acids with a molecular mass of ca. 133.5 kDa. The protein is identical to the native Cry1Ab protein produced by B. thuringiensis subsp. kurstaki HD-1, except for the additional 26 amino acids.
Detailed molecular analyses indicate that one intact copy of the flcry1Ab gene has been inserted at a single site in the plant genome, and is stably inherited and expressed from one generation to the next. No antibiotic resistance marker genes are present in COT67B cotton.
The FLCry1Ab protein is expressed at moderately low levels in cottonseed (25.17 μg/g dry weight), with quantifiable levels also being detected in linters (9.65 μg/g dry weight), and cottonseed meal (47.50 μg/g dry weight). No FLCry1Ab was detected in refined cottonseed oil.
EN
Detailed molecular analyses indicate that one intact copy of the flcry1Ab gene has been inserted at a single site in the plant genome, and is stably inherited and expressed from one generation to the next. No antibiotic resistance marker genes are present in COT67B cotton.
The FLCry1Ab protein is expressed at moderately low levels in cottonseed (25.17 μg/g dry weight), with quantifiable levels also being detected in linters (9.65 μg/g dry weight), and cottonseed meal (47.50 μg/g dry weight). No FLCry1Ab was detected in refined cottonseed oil.
The Cry1Ab protein has activity against several important pestiferous lepidopteran species of cotton including, but not limited to, Helicoverpa zea (cotton bollworm), Heliothis virescens (tobacco budworm), Ectinophora gossypiella (pink bollworm) and Trichoplusia ni (cabbage looper).
Cry proteins, of which Cry1Ab is only one, act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow, causing gut paralysis and eventual death from bacterial sepsis. Cry1Ab is lethal only when eaten by the larvae of lepidopteran insects, and its specificity of action is directly attributable to the presence of specific binding sites in the target insects. There are no binding sites for delta-endotoxins of B. thuringiensis on the surface of mammalian intestinal cells, therefore, livestock animals and humans are not susceptible to these proteins.
A large number of studies have been done with FLCry1Ab to confirm its identity and physicochemical and functional properties as well as to determine its potential toxicity and allergenicity. These studies have demonstrated that FLCry1Ab expressed in COT67B conforms in size and amino acid sequence to that expected, does not exhibit any post-translational modification including glycosylation, and exhibits the expected insecticidal activity.
EN
Cry proteins, of which Cry1Ab is only one, act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow, causing gut paralysis and eventual death from bacterial sepsis. Cry1Ab is lethal only when eaten by the larvae of lepidopteran insects, and its specificity of action is directly attributable to the presence of specific binding sites in the target insects. There are no binding sites for delta-endotoxins of B. thuringiensis on the surface of mammalian intestinal cells, therefore, livestock animals and humans are not susceptible to these proteins.
A large number of studies have been done with FLCry1Ab to confirm its identity and physicochemical and functional properties as well as to determine its potential toxicity and allergenicity. These studies have demonstrated that FLCry1Ab expressed in COT67B conforms in size and amino acid sequence to that expected, does not exhibit any post-translational modification including glycosylation, and exhibits the expected insecticidal activity.
- Draft Assessment Report - Cotton line COT67B.pdf [ English ]
- SYN-IR67B-1 - OECD [ English ]
- SYN-IR67B-1 - FDA [ English ]
| Record type | Field | Record(s) | |
|---|---|---|---|
| Country's Decision or any other Communication | LMO identification | 5 | |
| Risk Assessment generated by a regulatory process | Living modified organism(s) | 5 | |
| Living Modified Organism | Recipient Organism or Parental Organisms | 6 | |
