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Modified Organism
SYN-IR67B-1 - Insect-resistant cotton
Record information and status
Record ID
47352
Status
Published
Date of creation
2008-10-31 16:38 UTC (manoela.miranda@cbd.int)
Date of last update
2012-12-07 17:18 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-12-07 17:18 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Insect-resistant cotton
Transformation event
COT67B
Unique identifier
SYN-IR67B-1
Developer(s)
Syngenta
Url:Syngenta
Description
Cotton resistant to lepidopteran pests through introduction of the cry1Ab gene which codes for the Cry1Ab insecticidal protein that targets lepidopteran insect species.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Gossypium hirsutum - Cotton
Point of collection or acquisition of the recipient organism
Cultivar: Coker 312
Characteristics of the transformation process
Vector
pNOV4641 and pNOV1914
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Ti plasmid right border repeat
0.00 Kb
 
 
Actin 2 Gene Promoter
0.00 Kb
 
 
Cry1Ab
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
Ti plasmid left border repeat
0.00 Kb
 
 
Ti plasmid right border repeat
0.00 Kb
 
 
Ubiquitin gene 3 promoter
0.00 Kb
 
 
Hygromycin B phosphotransferase gene
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
Ti plasmid left border repeat
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The conventional cotton line Coker 312 was transformed by an Agrobacterium-mediated transformation using two binary vectors, pNOV4641 and pNOV1914. Once transformants were identified, Syngenta used traditional breeding techniques to select plants containing flcry1Ab but not the aph4 gene from pNOV1914 or elements from its plasmid backbone.

Genetic Elements in Plasmid pNOV4641:
Contains a synthetic full-length cry1Ab gene - designated flcry1Ab - originally derived from B. thuringiensis, subspecies kurstaki HD-1, which expresses the insecticidal FLCry1Ab protein

Genetic Elements in Plasmid pNOV1914:
Contains the aph4 gene derived from Escherichia coli, which expresses the enzyme hygromycin-B phosphotransferase for hygromycin resistance.   The aph4 gene is used as a selectable marker for the transformation process.

Information on the inserted DNA sequences (in Spanish):
El gen insertado en el algodón COT67B, cry1Ab confiere resistencia a lepidopteros al codificar la proteina Cry1Ab, el gen proviene de Bacillus thuringiensis subsp. kurstaki.

Se utilizó el sistema de transformación de A. tumefaciens. Los genes insertados en COT67B, junto con los componentes necesarios para su regulación en planta son: Gen cry1Ab que codifica la proteína Cry1Ab; Promotor Act2 de Arabidosis thaliana que confiere la expression de gen cry1Ab; nos 3' (terminador del gen principal); gen aph4 de E. coli (Marcador de selección) que fue descartado y no se encuentra en las plantas derivadas de COT67B; gen ubi3 de Arabidosis thaliana (promotor del gen marcador); nos 3' (terminador del gen marcador).
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Fiber / Textile
Detection method(s)
Additional information
COT67B cotton was generated through the transfer of the full length cry1Ab gene (flcry1Ab) to the conventional cotton line Coker 312.  The flcry1Ab gene, derived from B. thuringiensis subspecies kurstaki HD-1, has been modified to restore a 26 amino acid motif in the C-terminus of the protein which had been lost during a natural recombination event which generated the wild type cry1Ab gene.  The flcry1Ab gene encodes FLCry1Ab, a full-length Cry protein consisting of 1181 amino acids with a molecular mass of ca. 133.5 kDa.  The protein is identical to the native Cry1Ab protein produced by B. thuringiensis subsp. kurstaki HD-1, except for the additional 26 amino acids.

Detailed molecular analyses indicate that one intact copy of the flcry1Ab gene has been inserted at a single site in the plant genome, and is stably inherited and expressed from one generation to the next.  No antibiotic resistance marker genes are present in COT67B cotton.

The FLCry1Ab protein is expressed at moderately low levels in cottonseed (25.17 μg/g dry weight), with quantifiable levels also being detected in linters (9.65 μg/g dry weight), and cottonseed meal (47.50 μg/g dry weight).  No FLCry1Ab was detected in refined cottonseed oil.
Additional Information
Additional Information
The Cry1Ab protein has activity against several important pestiferous lepidopteran species of cotton including, but not limited to, Helicoverpa zea (cotton bollworm), Heliothis virescens (tobacco budworm), Ectinophora gossypiella (pink bollworm) and Trichoplusia ni (cabbage looper).

Cry proteins, of which Cry1Ab is only one, act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow, causing gut paralysis and eventual death from bacterial sepsis. Cry1Ab is lethal only when eaten by the larvae of lepidopteran insects, and its specificity of action is directly attributable to the presence of specific binding sites in the target insects. There are no binding sites for delta-endotoxins of B. thuringiensis on the surface of mammalian intestinal cells, therefore, livestock animals and humans are not susceptible to these proteins.

A large number of studies have been done with FLCry1Ab to confirm its identity and physicochemical and functional properties as well as to determine its potential toxicity and allergenicity. These studies have demonstrated that FLCry1Ab expressed in COT67B conforms in size and amino acid sequence to that expected, does not exhibit any post-translational modification including glycosylation, and exhibits the expected insecticidal activity.

Records referencing this document (16)
IDDescription
16record(s) found
Country's Decision or any other Communication5 records
Modified Organism6 records
Risk Assessment5 records