Record details

Living Modified Organism identity

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LMO name

Modified thermostable alpha-amylase, insect-resistant, herbicide-tolerant maize

Transformation event

3272 x Bt11 x MIR604 x GA21

Unique identifier

SYN-E3272-5 x SYN-BTØ11-1 x SYN-IR6Ø4-5 x MON-ØØØ21-9

Developer(s)

Record #38695

Syngenta Japan K.K.

401-2, Mukounodai, Takatsuhara, Tako-machi,

Katori-gun, Chiba

Japan, 289-2304

Url:	http://www.syngentaseeds.co.jp/

Description

The stacked maize line SYN-E3272-5, SYN-BTØ11-1, SYN-IR6Ø4-5 and MON-ØØØ21-9 was obtained through the traditional cross breading of each of the parental organisms to produce a maize that expresses each of amy797E alpha amylase, PMI, Cry1Ab, PAT, mCry3A and EPSPS genes. The expression of these genes are expected to confer resistance to Lepidoptera and Coleoptera, and tolerant to glufosinate and glyphosate herbicide as well as the synthesis of thermostable alpha-amylase.

Recipient Organism or Parental Organisms

The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.

Record #246

Zea mays - Maize, Corn, MAIZE

Record #15109

SYN-E3272-5 - Enogen™ Maize

Mannose tolerance Selectable marker genes and reporter genes Thermostable alpha-amylase Use in industrial applications - Biofuel production

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Record #14797

SYN-BTØ11-1 - YieldGard™ maize

Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate

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Record #15105

SYN-IR6Ø4-5 - Agrisure™ RW Rootworm-Protected maize

Mannose tolerance Resistance to diseases and pests - Insects - Coleoptera (beetles) - Western corn rootworm (Diabrotica virgifera) Selectable marker genes and reporter genes

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Record #14794

MON-ØØØ21-9 - Roundup Ready™ maize

Resistance to herbicides - Glyphosate

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Related LMOs

Record #43635

SYN-BTØ11-1 x SYN-IR6Ø4-5 x MON-ØØØ21-9 - Agrisure™ 3000GT Maize

Mannose tolerance Resistance to diseases and pests - Insects - Coleoptera (beetles) - Western corn rootworm (Diabrotica virgifera), Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate, Glyphosate Selectable marker genes and reporter genes

Show detection method(s)

Characteristics of the transformation process

Vector

pNOV7013, pZO1502, pZM26 and pDPG434

Techniques used for the modification

Cross breeding

Genetic elements construct

27kD gamma-zein Promoter

#103622

0.68 Kb

amy797E alpha amylase

#14966

1.38 Kb

SEKDEL ER retention signal

#102033

0.01 Kb

Phosphoenolpyruvate carboxylase, intron 9

#101406

0.11 Kb

CaMV 35S terminator

#100290

0.07 Kb

Ubiquitin gene promoter

#100362

1.99 Kb

Ubiquitin Intron 1

#103627

0.00 Kb

Phosphomannose Isomerase gene

#15003

1.18 Kb

Nopaline Synthase Gene Terminator

#100269

0.25 Kb

CaMV 35S promoter

#100287

0.51 Kb

Alcohol Dehydrogenase 1, intron 6

#103625

0.47 Kb

Cry1Ab

#14985

1.85 Kb

Nopaline Synthase Gene Terminator

#100269

0.25 Kb

CaMV 35S promoter

#100287

0.42 Kb

Alcohol Dehydrogenase 1, intron 2

#103867

0.18 Kb

Phosphinothricin N-acetyltransferase gene

#15002

0.55 Kb

Nopaline Synthase Gene Terminator

#100269

0.25 Kb

Metallothionein-like gene promoter

#103881

2.56 Kb

mCry3A

#43634

1.80 Kb

Nopaline Synthase Gene Terminator

#100269

0.25 Kb

Ubiquitin gene promoter

#100362

0.98 Kb

Ubiquitin Intron 1

#103627

1.01 Kb

Phosphomannose Isomerase gene

#15003

1.18 Kb

Nopaline Synthase Gene Terminator

#100269

0.25 Kb

Rice actin 1 gene promoter

#100364

1.37 Kb

Rice actin 1, intron

#100355

0.00 Kb

Optimized Transit Peptide

#101419

0.37 Kb

5-enolpyruvylshikimate-3-phosphate synthase

#15000

1.34 Kb

Nopaline Synthase Gene Terminator

#100269

0.24 Kb

Further details

Notes regarding the genetic elements introduced or modified in this LMO

DNA insert from 3272 vector pNOV7013
Maize containing thermostable alpha-amylase (for optimised bioethanol production) through introduction of the amy797E gene from Thermococcales (thermostable bacterium). The pmi gene expresses the PMI protein, which allows the transformed plants to use mannose as an energy source and is used as a selectable marker.

DNA insert from Bt11 vector pZO1502
Insect-resistant and herbicide tolerant maize produced by inserting the cry1Ab gene to confer resistance to the European corn borer (Ostrinia nubilalis), and the phosphinothricin N-acetyltransferase (PAT) encoding gene to confer tolerance to phosphinothricin (PPT) herbicide, specifically glufosinate ammonium.

DNA insert from MIR604 vector pZM26
MIR604 is a genetically modified to confer field protection against corn root worms. The cry3A gene codes for a Bt-toxin (Cry3A), which confers resistance to coleopteran species. Expression of the pmi gene allows the plant to use mannose as a carbon source through production of the PMI protein, and is used as a selectable marker.

DNA insert from GA21 vector pDPG434
The GA21 line of maize was modified to be tolerant of glyphosate-containing herbicides. The isolated endogenous maize epsps gene was modified through site-directed mutagenesis, such that its encoded enzyme was insensitive to inactivation by glyphosate, and inserted into the inbred AT maize variety.

For additional information on this LMO, please refer to the records of the parental LMOs.

LMO characteristics

Modified traits

Resistance to diseases and pests
- Insects
  - Coleoptera (beetles)
    - Western corn rootworm (Diabrotica virgifera)
  - Lepidoptera (butterflies and moths)
    - European corn borer (Ostrinia nubilalis)
Resistance to herbicides
- Glufosinate
- Glyphosate
Selectable marker genes and reporter genes
Use in industrial applications
- Biofuel production

Common use(s)

Food
Feed
Biofuel

Detection method(s)

External link(s)

SYN-E3272-5 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF)

SYN-BTØ11-1 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF)

SYN-IR6Ø4-5 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF)

MON-ØØØ21-9 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF)