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Modified Organism
SYN-E3272-5 x SYN-BTØ11-1 x SYN-IR6Ø4-5 x MON-ØØØ21-9 - Modified thermostable alpha-amylase, insect-resistant, herbicide-tolerant maize
Record information and status
Record ID
101287
Status
Published
Date of creation
2010-07-23 15:22 UTC (bch@env.go.jp)
Date of last update
2013-07-24 17:04 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-07-24 17:04 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Modified thermostable alpha-amylase, insect-resistant, herbicide-tolerant maize
Transformation event
3272 x Bt11 x MIR604 x GA21
Unique identifier
SYN-E3272-5 x SYN-BTØ11-1 x SYN-IR6Ø4-5 x MON-ØØØ21-9
Developer(s)
Syngenta Japan K.K.
401-2, Mukounodai, Takatsuhara, Tako-machi,
Katori-gun, Chiba
Japan, 289-2304
Url:http://www.syngentaseeds.co.jp/
Description
The stacked maize line SYN-E3272-5, SYN-BTØ11-1, SYN-IR6Ø4-5 and MON-ØØØ21-9 was obtained through the traditional cross breading of each of the parental organisms to produce a maize that expresses each of amy797E alpha amylase, PMI, Cry1Ab, PAT, mCry3A and EPSPS genes. The expression of these genes are expected to confer resistance to Lepidoptera and Coleoptera, and tolerant to glufosinate and glyphosate herbicide as well as the synthesis of thermostable alpha-amylase.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
SYN-E3272-5 - Enogen™ Maize
Mannose tolerance Selectable marker genes and reporter genes Thermostable alpha-amylase Use in industrial applications - Biofuel production
Show detection method(s)
SYN-BTØ11-1 - YieldGard™ maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate
Show detection method(s)
SYN-IR6Ø4-5 - Agrisure™ RW Rootworm-Protected maize
Mannose tolerance Resistance to diseases and pests - Insects - Coleoptera (beetles) - Western corn rootworm (Diabrotica virgifera) Selectable marker genes and reporter genes
Show detection method(s)
MON-ØØØ21-9 - Roundup Ready™ maize
Resistance to herbicides - Glyphosate
Show detection method(s)
Related LMOs
SYN-BTØ11-1 x SYN-IR6Ø4-5 x MON-ØØØ21-9 - Agrisure™ 3000GT Maize
Mannose tolerance Resistance to diseases and pests - Insects - Coleoptera (beetles) - Western corn rootworm (Diabrotica virgifera), Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate, Glyphosate Selectable marker genes and reporter genes
Show detection method(s)
Characteristics of the transformation process
Vector
pNOV7013, pZO1502,  pZM26 and pDPG434
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
27kD gamma-zein Promoter
0.68 Kb
 
 
amy797E alpha amylase
1.38 Kb
 
 
SEKDEL ER retention signal
0.01 Kb
 
 
Phosphoenolpyruvate carboxylase, intron 9
0.11 Kb
 
 
CaMV 35S terminator
0.07 Kb
 
 
Ubiquitin gene promoter
1.99 Kb
 
 
Ubiquitin Intron 1
0.00 Kb
 
 
Phosphomannose Isomerase gene
1.18 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
CaMV 35S promoter
0.51 Kb
 
 
Alcohol Dehydrogenase 1, intron 6
0.47 Kb
 
 
Cry1Ab
1.85 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
CaMV 35S promoter
0.42 Kb
 
 
Alcohol Dehydrogenase 1, intron 2
0.18 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Metallothionein-like gene promoter
2.56 Kb
 
 
mCry3A
1.80 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Ubiquitin gene promoter
0.98 Kb
 
 
Ubiquitin Intron 1
1.01 Kb
 
 
Phosphomannose Isomerase gene
1.18 Kb
 
 
Nopaline Synthase Gene Terminator
0.25 Kb
 
 
Rice actin 1 gene promoter
1.37 Kb
 
 
Rice actin 1, intron
0.00 Kb
 
 
Optimized Transit Peptide
0.37 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase
1.34 Kb
 
 
Nopaline Synthase Gene Terminator
0.24 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from 3272 vector pNOV7013
Maize containing thermostable alpha-amylase (for optimised bioethanol production) through introduction of the amy797E gene from Thermococcales (thermostable bacterium). The pmi gene expresses the PMI protein, which allows the transformed plants to use mannose as an energy source and is used as a selectable marker.

DNA insert from Bt11 vector pZO1502
Insect-resistant and herbicide tolerant maize produced by inserting the cry1Ab gene to confer resistance to the European corn borer (Ostrinia nubilalis), and the phosphinothricin N-acetyltransferase (PAT) encoding gene to confer tolerance to phosphinothricin (PPT) herbicide, specifically glufosinate ammonium.

DNA insert from MIR604 vector pZM26
MIR604 is a genetically modified to confer field protection against corn root worms. The cry3A gene codes for a Bt-toxin (Cry3A), which confers resistance to coleopteran species. Expression of the pmi gene allows the plant to use mannose as a carbon source through production of the PMI protein, and is used as a selectable marker.

DNA insert from GA21 vector pDPG434
The GA21 line of maize was modified to be tolerant of glyphosate-containing herbicides. The isolated endogenous maize epsps gene was modified through site-directed mutagenesis, such that its encoded enzyme was insensitive to inactivation by glyphosate, and inserted into the inbred AT maize variety.

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Food
  • Feed
  • Biofuel

Records referencing this document (8)
IDDescription
8record(s) found
Country's Decision or any other Communication3 records
Organization1 record
Risk Assessment4 records