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Modified Organism
BCS-GHØØ5-8 - Herbicide-tolerant and lepidoptera-resistant cotton
Record information and status
Record ID
101898
Status
Published
Date of creation
2011-06-10 08:16 UTC (protocolo.cartagena@magrama.es)
Date of last update
2013-01-28 16:09 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-01-28 16:09 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Herbicide-tolerant and lepidoptera-resistant cotton
Transformation event
GHB119
Unique identifier
BCS-GHØØ5-8
Developer(s)
Bayer BioScience N.V.
Bayer BioScience N.V.
Technologiepark 38
Gent
Belgium, B-9052
Description
The introduced lepidoptera resistance trait in GHB119 cotton is conferred by the insecticidal crystal protein, Cry2Ae, encoded by the cry2Ae gene, from the common soil bacterium, Bacillus thuringiensis subsp. dakota (B.t. dakota). The Cry2Ae protein is effective in controlling lepidopteran plant feeding larvae such as cotton bollworm larvae (CBW, Helicoverpa zea), tobacco budworm larvae (TBW, Heliothis virescens) and fall armyworm larvae (FAW, Spodoptera frugiperda), which are common pests of cotton.

The herbicide tolerance trait (OECD, 1999 M-204493-01-1) is based upon the bar gene, a bialaphos resistance gene, isolated from the soil microorganism, Streptomyces hygroscopicus. The bar gene, when expressed, enables the production of the enzyme, Phosphinothricin-Acetyl-Transferase (PAT) that acetylates L-glufosinate ammonium and thereby confers tolerance to herbicides based upon glufosinate ammonium.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Gossypium hirsutum - Cotton
Characteristics of the transformation process
Vector
pTEM12 derived from pGSC1700
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Ti plasmid left border repeat
0.02 Kb
 
 
Nopaline Synthase Gene Terminator
0.31 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
CsVMV promoter
0.54 Kb
 
 
CaMV 35S promoter
0.48 Kb
 
 
5' untranslated leader of chlorophyll a/b-binding protein
0.07 Kb
 
 
rbcS Transit Peptide
0.16 Kb
 
 
Cry2Ae
1.90 Kb
 
 
CaMV 35S terminator
0.27 Kb
 
 
Ti plasmid right border repeat
0.02 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Southern Blot analysis indicated that a single copy of the insert is present in the GHB119 line and a configuration that correspond to that in the original vector. No insertion of plasmid backbone sequences were detected, however three ORF were present at the junctions of the integration site but do not have the required regulatory elements for functionality.

The vector pTEM12 is derived from pGSC1700 which was constructed in E. coli and thereafter transferred to a suitable Agrobacterium tumefaciens strain.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Fiber / Textile
  • Industrial
Detection method(s)
Additional Information

Records referencing this document (25)
IDDescription
25record(s) found
Country's Decision or any other Communication8 records
Modified Organism7 records
Organization2 records
Risk Assessment8 records