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Modified Organism
Insect resistant cotton
Record information and status
Record ID
103216
Status
Published
Date of creation
2012-03-20 11:56 UTC (warrier@nic.in)
Date of last update
2016-05-17 16:55 UTC (dina.abdelhakim@cbd.int)
Date of publication
2016-05-17 16:55 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Insect resistant cotton 
Transformation event
Event1
Developer(s)
Mr S K Gupta
President
JK Agri Genetics Ltd
1-10-177, 4th Floor, Varun Towers
Begumpet
Hyderabad, Andhra Pradesh
India, 500016
Phone:+91 40 55316858
Fax:+91 40 27764943
Email:info@jkseeds.net
Url:JK seeds website
Description
This modified cotton contains the insecticidal cry1Ac gene which imparts resistance against Lepidoptera. The LMO also contains the selectable markers nptII to isolate transformed seedlings and a GUS gene cassette as a reporter gene.

Bt-cotton was generated by using the biolistic method of transformation system. The transformed cotton shoots containing the nptII gene were selected on medium supplemented with kanamycin.  A procedure for biolistics method of transformation of cotton is novel and performed using shoot meristem. Plants were regenerated and ultimately plantlets were grown in soil and assayed for insect resistance.

The cry1Ac gene in Indian inbred lines behave as a single dominant Mendelian factor and is stably integrated in the plant genome.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Gossypium hirsutum - Cotton
Point of collection or acquisition of the recipient organism
Local collection, India
Characteristics of the transformation process
Vector
pUC18
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
CaMV Enhanced 35S promoter
0.00 Kb
 
 
Cry1Ac
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
 
CaMV 35S promoter
0.00 Kb
 
 
Neomycin Phosphotransferase II
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
A truncated cry1Ac gene derived from Bacillus thuringiensis subsp. kurstaki (B.t.k.)
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Fiber / Textile
Detection method(s)
Additional information
Immunological and DNA based detection methods have been developed for cry1Ac event-1 its expressed protein. ELISA kits from Envirologix, USA are being used for detecting Cry1Ac protein in our laboratory. The gene specific primers designed and supplied by BREF IIT Kharagpur are being used for detecting this event. Central Institute of Cotton Research, ICAR, Nagpur, India, is the referral laboratory for event testing. RT-PCR based LOD (Limit of Detection) protocol was developed and validated at SGS, Ahmedabad, India.     
Additional Information
Other relevant website address or attached documents

Records referencing this document (6)
IDDescription
6record(s) found
Country's Decision or any other Communication3 records
Organization1 record
Risk Assessment2 records