Immunological and DNA based detection methods have been developed
for cry1Ac event-1 its expressed protein. ELISA kits from
Envirologix, USA are being used for detecting Cry1Ac protein in our
laboratory. The gene specific primers designed and supplied by BREF
IIT Kharagpur are being used for detecting this event. Central
Institute of Cotton Research, ICAR, Nagpur, India, is the referral
laboratory for event testing. RT-PCR based LOD (Limit of Detection)
protocol was developed and validated at SGS, Ahmedabad, India.