Maize modified for insect resistance and herbicide tolerance

The stacked maize line was produced through conventional breeding technique of crossing the Herculex I corn event TC1507 with MON810 maize. The cross is insect-resistant maize, tolerant to the herbicide glufosinate ammonium.

PHI8999A, PV-ZMBK07 and PV-ZMGT10

DNA insert from MON810, vectors PV-ZMBK07 and PV-ZMGT10:

MON810 contains a truncated portion of a synthetic form of the cry1Ab gene from Bacillus thuringiensis subsp. Two constructs PV-ZMBK07 and PV-ZMGT10 have been used for transformation, but molecular analyses showed that MON810 does not contain any element from PV-ZMGT10 construct and only the elements from construct PV-ZMBK07 have been integrated into its genome.

DNA insert from TC1507, vector PHI8999A: 

DNA fragment PHI8999A contains two adjacent plant gene expression cassettes for the cry1F and pat genes

For additional information on this LMO, please refer to the records of the parental LMOs.

MON810 contains one integrated DNA consisting of P-e35S, I-Hsp70 and cry1Ab. The terminator of the nopaline synthase (nos) gene was lost due to a truncation at the 3' end of the gene cassette during genome integration and is, therefore, not present in MON810.

Southern analysis using a cry1F probe carried out by the European Food Safety Authority revealed the presence of two cry1F inserts. The first represented the intact gene from the expression cassette. The second insert was a truncated cry1F  fragment of 335bp, which is located at the 5' end of the insertion locus. In addition, analysis of the sequences adjacent to the insert of fragment PHI8999A revealed DNA fragments that correspond to small segments from PHI8999A, including incomplete sequences from the pat gene, the maize ubiquitin promoter and the terminator from Agrobacterium. Furthermore, different fragments of chloroplast DNA and a number of sequences with similarity to retrotransposons are also present in the border region of the insert.