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Modified Organism
DAS-Ø15Ø7-1 x MON-ØØ81Ø-6 - Maize modified for insect resistance and herbicide tolerance
Record information and status
Record ID
103256
Status
Published
Date of creation
2012-03-27 14:00 UTC (gutemberg.sousa@mctic.gov.br)
Date of last update
2013-07-24 18:34 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-07-24 18:34 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Maize modified for insect resistance and herbicide tolerance
Transformation event
TC1507 x MON810
Unique identifier
DAS-Ø15Ø7-1 x MON-ØØ81Ø-6
Developer(s)
Msc Goran Kuhar Jezovsek
Gerente de Regulamentação e Registros
Regulamentação e Registro
Du Pont do Brasil S.A. (Du Pont - Divisão Pioneer Sementes)
Du Pont do Brasil S.A. - Divisão Pioneer Sementes
Endereço: SGAS 902 Lt 74 Cj B Sala 221 a 224
Bloco A - Ed. Athenas - Asa Sul
Brasília, Distrito Federal
Brazil, 70390-020
Phone:55 - 61 - 21039111
Fax:55 - 61 -21039111
Email:goran.kuhar@pioneer.com
Url:Pioneer Sementes
Description
The stacked maize line was produced through conventional breeding technique of crossing the Herculex I corn event TC1507 with MON810 maize. The cross is insect-resistant maize, tolerant to the herbicide glufosinate ammonium.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
DAS-Ø15Ø7-1 - Herculex™ I maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths) Resistance to herbicides - Glufosinate
Show detection method(s)
MON-ØØ81Ø-6 - YieldGard™ maize
Resistance to diseases and pests - Insects - Lepidoptera (butterflies and moths)
Show detection method(s)
Characteristics of the transformation process
Vector
PHI8999A, PV-ZMBK07 and PV-ZMGT10
Techniques used for the modification
  • Cross breeding
Genetic elements construct
 
CaMV Enhanced 35S promoter
0.61 Kb
 
 
Hsp70 intron
0.80 Kb
 
 
Cry1Ab
3.46 Kb
 
 
Ubiquitin gene promoter
1.98 Kb
 
 
Cry1F
1.82 Kb
 
 
ORF25 PolyA Terminator sequence
0.72 Kb
 
 
CaMV 35S promoter
0.55 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
CaMV 35S terminator
0.20 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
DNA insert from MON810, vectors PV-ZMBK07 and PV-ZMGT10:

MON810 contains a truncated portion of a synthetic form of the cry1Ab gene from Bacillus thuringiensis subsp. Two constructs PV-ZMBK07 and PV-ZMGT10 have been used for transformation, but molecular analyses showed that MON810 does not contain any element from PV-ZMGT10 construct and only the elements from construct PV-ZMBK07 have been integrated into its genome.

DNA insert from TC1507, vector PHI8999A: 

DNA fragment PHI8999A contains two adjacent plant gene expression cassettes for the cry1F and pat genes

For additional information on this LMO, please refer to the records of the parental LMOs.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Detection method(s)
Additional information
MON810 contains one integrated DNA consisting of P-e35S, I-Hsp70 and cry1Ab. The terminator of the nopaline synthase (nos) gene was lost due to a truncation at the 3' end of the gene cassette during genome integration and is, therefore, not present in MON810.

Southern analysis using a cry1F probe carried out by the European Food Safety Authority revealed the presence of two cry1F inserts. The first represented the intact gene from the expression cassette. The second insert was a truncated cry1F  fragment of 335bp, which is located at the 5' end of the insertion locus. In addition, analysis of the sequences adjacent to the insert of fragment PHI8999A revealed DNA fragments that correspond to small segments from PHI8999A, including incomplete sequences from the pat gene, the maize ubiquitin promoter and the terminator from Agrobacterium. Furthermore, different fragments of chloroplast DNA and a number of sequences with similarity to retrotransposons are also present in the border region of the insert.
Additional Information
Other relevant website address or attached documents

Records referencing this document (33)
IDDescription
33record(s) found
Country's Decision or any other Communication14 records
Information Resource1 record
Organization3 records
Risk Assessment15 records