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Modified Organism
IFD-264Ø7-2 - Moonvelvet carnation
Record information and status
Record ID
104609
Status
Published
Date of creation
2013-01-14 23:01 UTC (schandler@florigene.com.au)
Date of last update
2014-08-28 16:55 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-08-28 16:55 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Moonvelvet carnation
Transformation event
26407
Unique identifier
IFD-264Ø7-2
Developer(s)
Dr Yukihisa Katsumoto
Principal Researcher
Research Institute
Suntory Holdings Limited (SHD)
1-1-1 Wakayamadai, Shimamoto-cho
Mishima-gun, Osaka
Japan, 618-8503
Phone:+81 75 962 9132
Fax:+81 75 962 3791
Email:Yoshikazu_Tanaka@suntory.co.jp,Yukihisa_Katsumoto@suntory.co.jp
Description
Moonvelvet is a flower colour modified carnation variety produced through introduction of the anthocyanin biosynthetic gene flavonoid 3',5'hydroxylase (F3'5'H) from Petunia hybrida to result in a violet/mauve colouration and the cytb5 gene to enhance the activity of flavonoid 3',5'-hydroxylase. A variant form of acetohydroxy acid/ acetolactate synthase (ALS) from Nicotiana tabacum was used as a selectable marker to confer tolerance to sulfonylurea herbicide.

Flowers of the transgenic plant produce delphinidin-related anthocyanins, not found in nature in carnation.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Dianthus caryophyllus - Carnation, DIACA
Characteristics of the transformation process
Vector
pCGP 2355
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.19 Kb
 
 
5' untranslated leader of chlorophyll a/b-binding protein
0.06 Kb
 
 
Acetohydroxy acid synthase gene
1.99 Kb
 
 
Acetohydroxy acid synthase gene terminator
1.77 Kb
 
 
Chalcone synthase gene promoter
1.17 Kb
 
 
Cytochrome b5
0.46 Kb
 
 
D8 gene terminator
0.82 Kb
 
 
Anthocyanidin synthase gene promoter
2.41 Kb
 
 
Flavonoid 3', 5' hydroxylase gene
1.52 Kb
 
 
Anthocyanidin synthase gene terminator
0.81 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The T-DNA is present at one integration locus and contains one copy of each T-DNA component as determined by Southern blot analysis
LMO characteristics
Modified traits
Common use(s)
  • Ornamental

Records referencing this document (12)
IDDescription
12record(s) found
Country's Decision or any other Communication6 records
Risk Assessment6 records