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Modified Organism
ACS-BNØ11-5 - Navigator™ canola
Record information and status
Record ID
14752
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2012-08-14 18:30 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-08-14 18:30 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Navigator™ canola
Transformation event
Oxy-235
Unique identifier
ACS-BNØ11-5
Developer(s)
Bayer CropScience (Aventis (Rhône Poulenc))
Url:Bayer CropScience
Description
Line Oxy-235 was developed to allow the use of oxynil herbicides, ioxynil and bromoxynil, as weed control options in canola. Canola tolerant to the oxynil herbicides created through insertion of the bxn gene isolated from the bacterium Klebsiella ozaenae, encoding a nitrilase enzyme that hydrolyzes oxynil herbicides to non-phytotoxic compounds. Bromoxynil is very effective on broadleaf weeds that are common in canola fields and the deployment of transgenic bromoxynil-tolerant canola will allow the post-emergence control of these weeds without crop injury.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA
Point of collection or acquisition of the recipient organism
Canola variety Westar
Characteristics of the transformation process
Vector
pRPA-BL-150a
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.00 Kb
 
 
RuBisCO small subunit gene 5'UTR
0.00 Kb
 
 
Bromoxynil-specific nitrilase
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Transgenic Oxy-235 contains a single copy of the bxn gene isolated from the bacterium Klebsiella pneumoniae (subsp. ozaenae), which encodes a nitrilase enzyme that hydrolyzes oxynil herbicides to non-phytotoxic compounds. Constitutive expression of the bxn gene was regulated via the 35S promoter from cauliflower mosaic virus (CaMV) and levels of nitrilase enzyme were detected in samples of leaf (1000 ng/mg total protein) and seed (<10 ng/mg total protein) tissue. No nitrilase was detectable in samples of refined oil (detection limit 20 ppb), and it was concluded that there will be no human exposure to this proteins.
LMO characteristics
Modified traits

Records referencing this document (12)
IDDescription
12record(s) found
Country's Decision or any other Communication7 records
Organization2 records
Risk Assessment3 records