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Modified Organism
ACS-BNØØ8-2 - Herbicide Tolerant Canola
Record information and status
Record ID
14762
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-05-30 13:54 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-05-30 13:54 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Herbicide Tolerant Canola
Transformation event
T45 (HCN28)
Unique identifier
ACS-BNØØ8-2
Developer(s)
Description
Canola  modified for glufosinate tolerance with the insertion of the phosphinothricin acetyltransferase (pat) gene from Streptomyces viridochromogenes, conferring tolerance to phosphinothricin (Glufosinate ammonium) herbicide.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA
Related LMOs
ACS-BNØØ7-1 - Liberty-Link™ Innovator Canola
Resistance to antibiotics - Kanamycin Resistance to herbicides - Glufosinate
Show detection method(s)
Characteristics of the transformation process
Vector
pHoe4/Ac
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.53 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
CaMV 35S terminator
0.22 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The pat gene is a synthetic version of the gene isolated from Streptomyces viridochromogenes, strain Tü 494. The nucleotide sequence has been modified to provide codons preferred by plants without changing the amino acid sequence of the enzyme.

Southern blot analysis indicated that a single copy of the transformation cassette was integrated into the host genome with no integration of any portions of the vector backbone.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Additional Information
The canola line T45 was genetically engineered to express tolerance to glufosinate ammonium, the active ingredient in phosphinothricin herbicides (Basta®, Rely®, Finale®, and Liberty®). Glufosinate chemically resembles the amino acid glutamate and acts to inhibit an enzyme, called glutamine synthetase, which is involved in the synthesis of glutamine. Essentially, glufosinate acts enough like glutamate, the molecule used by glutamine synthetase to make glutamine, that it blocks the enzyme's usual activity. Glutamine synthetase is also involved in ammonia detoxification. The action of glufosinate results in reduced glutamine levels and a corresponding increase in concentrations of ammonia in plant tissues, leading to cell membrane disruption and cessation of photosynthesis resulting in plant withering and death.

Glufosinate tolerance in T45 is the result of introducing a gene encoding the enzyme phosphinothricin-N-acetyltransferase (PAT) isolated from the common aerobic soil actinomycete, Streptomyces viridochromogenes, the same organism from which glufosinate was originally isolated. The PAT enzyme catalyzes the acetylation of phosphinothricin, detoxifying it into an inactive compound. The PAT enzyme is not known to have any toxic properties. Line HCN28 was derived from T45.
Other relevant website address or attached documents

Records referencing this document (33)
IDDescription
33record(s) found
Country's Decision or any other Communication12 records
Modified Organism2 records
Organization12 records
Risk Assessment7 records