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Modified Organism
MON-ØØ863-5 - YieldGard™ Rootworm™ maize
Record information and status
Record ID
14778
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-04-19 14:06 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-04-19 14:06 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
YieldGard™ Rootworm™ maize
Transformation event
MON863
Unique identifier
MON-ØØ863-5
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
Maize was modified for resistance to corn root worm by inserting the cry3Bb1 gene. A neomycin phosphotransferase II (npt II) gene was also integrated into the host genome and confers resistance to the antibiotic kanamycin.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Point of collection or acquisition of the recipient organism
From inbred line A634
Characteristics of the transformation process
Vector
PV-ZMIR13
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
CaMV 35S promoter
0.35 Kb
 
 
Neomycin Phosphotransferase II
0.97 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
 
CaMV 35S promoter plus four repeats of activating sequence
0.22 Kb
 
 
5' untranslated leader from chlorophyll a/b-binding protein
0.06 Kb
 
 
Rice actin 1, intron
0.49 Kb
 
 
Cry3Bb1
1.96 Kb
 
 
Terminator of the wheat heat shock protein 17.3
0.23 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Maize line MON 863 was produced by biolistic transformation of the inbred line A634 using linearized plasmid PV-ZMIR13 DNA purified following Mlu I restriction endonuclease digestion. The introduced DNA contained the modified cry3Bb1 gene from B. thuringiensis subsp. kumamotoensis. The modified cry3Bb1 gene encodes a protein of 653 amino acids whose amino acid sequence differs from that of the wild-type protein by the addition of an alanine residue at position 2 and by seven amino acid changes.

The introduced DNA also contained a copy of the neomycin phosphotransferase II (NPTII) encoding gene (nptII) derived from the Tn5 transposon of Escherichia coli. Due to the use of a unique restriction site for the excision of nptII from Tn5, this gene cassette also contains a 153 bp of the 378 bp bleomycin binding protein gene (ble).This segment of ble is located 20 nucleotides downstream of the nptII stop codon, and it is joined to the T-nos.

The mRNA that is transcribed from the nptII cassette contains tandem open reading frames (ORF). The proximal ORF is the complete nptII coding sequence while the distal ORF encodes approximately 40% of the bleomycin binding sequence. Due to differences in the mechanism of initiation of translation between procaryotic and eucaryotic organisms, it is highly unlikely that the partial ble ORF will be translated into protein in Mon863. This means that nptII will be expressed in Mon863, but the ble fragment will not. According to the US FDA, if the partial ble gene were translated into protein, the truncated peptide would not dimerize because it lacks the necessary amino acids to dimerize, and also lacks approximately 50% of the residues that are involved in bleomycin binding.

Molecular analyses of the transformed plant show that one DNA insert has been transferred to the genome of Mon863. This insert contains one copy of the Mlu I plasmid fragment used in transformation. Both cassettes are intact and no DNA from plasmid backbone was detected.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Biofuel
Additional Information

Records referencing this document (57)
IDDescription
57record(s) found
Country's Decision or any other Communication18 records
Information Resource1 record
Modified Organism3 records
Organization21 records
Risk Assessment14 records