| | english | español | français |
Go to record ID

  Home|Finding Information|Record details   Printer-friendly version

Modified Organism
CGN-89465-2 - High oleic acid canola
Record information and status
Record ID
14782
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2012-11-13 14:39 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-11-13 14:39 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
High oleic acid canola
Transformation event
23-198 (Event 23)
Unique identifier
CGN-89465-2
Developer(s)
Monsanto (Calgene)
Url:Monsanto Homepage
Description
The canola line 23-198 was genetically engineered to express modified seed fatty acid content, specifically high levels of lauric acid and myristic acid. The increased levels of lauric acid in oil from the modified canola lines allow for its use as a replacement for other lauric acid oils, such as coconut and palm kernel oil, in products such as confectionery coatings and fillings, margarines, spreads, shortenings and commercial frying oils.

The modified fatty acid content of 23-198 is a result of the insertion of a thioesterase (TE) encoding gene from the California bay tree, Umbellularia californica, which is an alternative source of the spice "bay leaf" that is harvested commercially from Lauris nobilis. The introduced thioesterase enzyme is active in the fatty acid biosynthetic pathway of the developing seed and causes the accumulation of triacylglycerides containing esterified lauric acid and, to a lesser extent, myristic acid.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Brassica napus - Turnip, Rapeseed, Canola Plant, Oilseed Rape, Rape, BRANA
Related LMOs
CGN-89111-8 - High oleic acid canola
Production of medical or pharmaceutical compounds (human or animal) - Omega-3 fatty acids (e.g. DHA) Resistance to antibiotics - Kanamycin
Characteristics of the transformation process
Vector
pCGN3828-212/86-23
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Napin gene promoter
0.00 Kb
 
 
Thioesterase
0.00 Kb
 
 
Napin gene terminator
0.00 Kb
 
 
CaMV 35S promoter
0.00 Kb
 
 
Neomycin Phosphotransferase II
0.00 Kb
 
 
Tumour Morphology Large gene terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The original transformation event 23 was estimated to have 15 copies of the genes, at five independent genetic loci, as shown by Southern and segregation analyses. Lines 23-198 and 23-18-17 are several generations removed from the original transformant. Mendelian inheritance, and Southern and PCR analyses of third-generation material show the stability of the introduced genes as the bands did not change position. Some of the copies are segregating out, as expected, so that these lines may not be homogeneous for the number of copies present.
LMO characteristics
Modified traits
Additional Information
Other relevant website address or attached documents

Records referencing this document (6)
IDDescription
6record(s) found
Country's Decision or any other Communication3 records
Modified Organism1 record
Risk Assessment2 records