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Modified Organism
NMK-89761-6 - Atlantic NewLeaf™ potato
Record information and status
Record ID
14789
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-05-02 14:02 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-05-02 14:02 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Atlantic NewLeaf™ potato
Transformation event
ATBT04-6
Unique identifier
NMK-89761-6
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
The transgenic cultivars of 'Atlantic' (ATBT04-6, ATBT04-27, ATBT04-30, ATBT04-31, ATBT04-36) NewLeaf® potatoes were genetically engineered to be resistant to attack by Colorado potato beetle (CPB; Leptinotarsa decemlineata) by producing their own insecticide. These lines were developed by introducing the cry3A gene, isolated from the common  soil bacterium Bacillus thuringiensis subspecies tenebrionis (Btt), into the potato genome.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Point of collection or acquisition of the recipient organism
Cultivar: Atlantic
Related LMOs
NMK-89279-1 - Atlantic NewLeaf™ potato
Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Insects - Coleoptera (beetles)
NMK-89367-8 - Atlantic NewLeaf™ potato
Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Insects - Coleoptera (beetles)
NMK-89613-2 - Atlantic NewLeaf™ potato
Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Insects - Coleoptera (beetles)
NMK-8917Ø-9 - Atlantic NewLeaf™ potato
Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Insects - Coleoptera (beetles)
Characteristics of the transformation process
Vector
PV-STBT04
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
rbcS Promoter
1.70 Kb
 
 
Cry3A
1.80 Kb
 
 
rbcS-E9 gene terminator
0.63 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
 
Neomycin Phosphotransferase II
0.79 Kb
 
 
CaMV 35S promoter
0.32 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The coding sequence of the Cry3A gene was modified to plant preferred codons. This resulted in changes to 399 of 1791 nucleotides but there were no changes to the resulting amino acid sequence.

Southern blot analysis indicated that a three copies of the transformation cassette for the coding sequences of both Cry3A and nptII were transformed into the host genome at 3 different sites. No portions of the vector backbone were detected.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Additional Information
The Cry3A protein expressed in these transgenic potato cultivars is identical to that found in nature and in commercial Bt spray formulations. Cry proteins, of which Cry3A is only one, act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow causing gut paralysis and eventual death due to bacterial sepsis. Cry3A is insecticidal only when eaten by the larvae of coleopteran insects such as Colorado potato beetle and its specificity of action is directly attributable to the presence of specific binding sites in the target insects.
Other relevant website address or attached documents

Records referencing this document (8)
IDDescription
8record(s) found
Country's Decision or any other Communication3 records
Modified Organism4 records
Risk Assessment1 record