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Modified Organism
NMK-89812-3 - New Leaf™ Russet Burbank potato
Record information and status
Record ID
14790
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-04-25 20:10 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-04-25 20:10 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
New Leaf™ Russet Burbank potato
Transformation event
BT06 (RBBT06)
Unique identifier
NMK-89812-3
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
Colorado potato beetle resistant potatoes produced by inserting the cry3A gene from Bacillus thuringiensis. The neomycin phosphotransferase II (npt II) gene confers resistance to the antibiotic kanamycin is used as a selection marker.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum tuberosum - Potato, SOLTU
Point of collection or acquisition of the recipient organism
Cultivar: Russet Burbank
Related LMOs
NMK-89675-1 - New Leaf™ Russet Burbank potato
Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Insects - Coleoptera (beetles)
NMK-89167-6 - New Leaf™ Russet Burbank potato
Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Insects - Coleoptera (beetles)
NMK-89175-5 - New Leaf™ Russet Burbank potato
Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Insects - Coleoptera (beetles)
NMK-89593-9 - New Leaf™ Russet Burbank potato
Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Insects - Coleoptera (beetles)
NMK-896Ø1-8 - New Leaf™ Russet Burbank potato
Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Insects - Coleoptera (beetles)
NMK-899Ø6-7 - New Leaf™ Russet Burbank potato
Resistance to antibiotics - Kanamycin Resistance to diseases and pests - Insects - Coleoptera (beetles)
Characteristics of the transformation process
Vector
PV-STBT02
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV Enhanced 35S promoter
0.62 Kb
 
 
Cry3A
1.80 Kb
 
 
rbcS-E9 gene terminator
0.63 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
 
Neomycin Phosphotransferase II
0.79 Kb
 
 
CaMV 35S promoter
0.32 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The coding sequence of the Cry3A gene was modified to plant preferred codons. This resulted in changes to 399 of 1791 nucleotides but there were no changes to the resulting amino acid sequence.

Southern blot analysis indicated that a single insert of the transformation cassette was integrated into the host genome with no detectable rearrangements, insertions or segments of the vector backbone.
LMO characteristics
Modified traits
Common use(s)
  • Food
Additional Information
Additional Information
Cry proteins, of which Cry3A is only one, act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow causing gut paralysis and eventual death due to bacterial sepsis. Cry3A is insecticidal only when eaten by the larvae of coleopteran insects such as Colorado potato beetle and its specificity of action is directly attributable to the presence of specific binding sites in the target insects.
Other relevant website address or attached documents

Records referencing this document (16)
IDDescription
16record(s) found
Country's Decision or any other Communication6 records
Modified Organism6 records
Risk Assessment4 records