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Modified Organism
MON-89383-1 - Roundup Ready™ cotton
Record information and status
Record ID
14822
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2012-12-11 15:27 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-12-11 15:27 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Roundup Ready™ cotton
Transformation event
1698
Unique identifier
MON-89383-1
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
Cotton tolerant to the herbicide glyphosate through incorporation of the epsps gene encoding 5-enolpyruvylshikimaete-3-phosphate synthase (EPSPS), that confers tolerance to the herbicide glyphosate. The neomycin phosphotransferase II (npt II) gene confers resistance to the antibiotic kanamycin.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Gossypium hirsutum - Cotton
Point of collection or acquisition of the recipient organism
Variety: Coker 312
Related LMOs
MON-Ø1445-2 - Roundup Ready™ cotton
Resistance to antibiotics - Kanamycin, Streptomycin Resistance to herbicides - Glyphosate
Show detection method(s)
Characteristics of the transformation process
Vector
PV-GHGT06
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
FMV 35S promoter
0.57 Kb
 
 
Chloroplast transit peptide 2
0.23 Kb
 
 
5-enolpyruvylshikimate-3-phosphate synthase gene
1.36 Kb
 
 
rbcS-E9 gene terminator
0.63 Kb
 
 
CaMV 35S promoter
0.32 Kb
 
 
Neomycin Phosphotransferase II
0.79 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Information on the inserted DNA sequences
Molecular characterization demonstrates that one T-DNA (transferred) insert was integrated into the cotton genome to produce cotton 1698.  The insert contains the CoMV promoter region, cp4epsps, nptII, aad (that is not expressed in plants) and a portion of ori-V coding regions. The cp4epsps gene encodes the naturally glyphosate tolerant EPSPS protein, the target site for glyphosate action. The nptII gene which is isolated from the Tn5 transposon encodes neomycin phosphotranferase type II (NPTII) which confers resistance to the aminoglycoside antibiotic kanamycin and neomycin. The nptII gene functions as a selectable marker and is driven by Cauliflower Mosaic Virus 35S promoter and is followed by a nopaline synthase (nos) 3' region which directs polyadenylation of the messenger RNA (mRNA). The aad gene encodes the bacterial selectable marker enzyme 3"(9) -O-aminoglycoside adenyltransferase (AAD) which allows for the selection of the bacteria containing  the PV-GHGT06 to grow on medium with spectinomycin or streptomycin. This aad gene is not expressed in Roundup Ready 1698.

Vector information
The plasmid vector PV-GHGT06 contains well characterized DNA segments required for selection and replication of the plasmid in the bacteria as well as a right border for initiating the region of T-DNA,  into the plant genomic DNA. The plant expression vectors were assembled, transformed in E. coli and mated into the ABI Agrobacterium strain by the triparental conjugation system using the helper plasmid pRK2013. The binary ABI strain contains the disarmed (lacking the T-DNA phytohormone genes) pTiC58 plasmid pMP90RK in a choramphenicol-resistant derivative of the A. tumefaciens strain A208. The disarmed pMP90RK Ti plasmid does not carry the T-DNA phytohormone genes and is unable to grow the crown gall disease.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Fiber / Textile
Detection method(s)
Additional information
Southern blot analysis indicated that there is a single locus into which the T-DNA integrated and that DNA contain 2 copies of the EPSPS coding sequence
Additional Information
Additional Information
Cotton line 1698 was genetically engineered to express resistance to glyphosate, the active ingredient in the herbicide Roundup®, allowing for its use as a weed control option. In order to obtain field tolerance to glyphosate herbicide, a bacterial gene encoding a glyphosate-tolerant form of the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) was introduced into the cotton genome by Agrobacterium-mediated transformation.

The EPSPS enzyme is present in all plants, bacteria, and fungi, and is part of an important biochemical pathway called the shikimate pathway, which is involved in the production of aromatic amino acids and other aromatic compounds. When conventional cotton plants are treated with glyphosate, the plants cannot produce the aromatic amino acids needed to grow and survive. EPSPS is not present in mammals, birds or aquatic life forms, which do not synthesize their own aromatic amino acids. For this reason, glyphosate has little toxicity to these organisms. The EPSPS enzyme is naturally present in foods derived from plant and microbial sources.

Records referencing this document (4)
IDDescription
4record(s) found
Country's Decision or any other Communication2 records
Modified Organism1 record
Risk Assessment1 record