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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Roundup Ready™ cotton
EN
1698
Yes
MON-89383-1
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Organization:Monsanto ()800 North Lindbergh Blvd.St. Louis, MO
63167, United States of AmericaPhone: + 1 314 694-1000,Fax: +1 314 694-3080,Email:Website: http://www.monsanto.com,
Cotton tolerant to the herbicide glyphosate through incorporation of the epsps gene encoding 5-enolpyruvylshikimaete-3-phosphate synthase (EPSPS), that confers tolerance to the herbicide glyphosate. The neomycin phosphotransferase II (npt II) gene confers resistance to the antibiotic kanamycin.
EN
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12080-6 Organism Gossypium hirsutum (Cotton)Crops
Variety: Coker 312
EN
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MON-Ø1445-2 - Roundup Ready™ cotton| Monsanto | Resistance to antibiotics (Kanamycin, Streptomycin), Resistance to herbicides (Glyphosate)
PV-GHGT06
EN
- Agrobacterium-mediated DNA transfer
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0.570 kb
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0.230 kb
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1.360 kb
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0.630 kb
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0.320 kb
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0.790 kb
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0.260 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Protein coding sequence | Resistance to herbicides (Glyphosate)
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-101507-5 FMV 34S promoter | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)Promoter
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BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Transit signal
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BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)Terminator
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
Information on the inserted DNA sequences
Molecular characterization demonstrates that one T-DNA (transferred) insert was integrated into the cotton genome to produce cotton 1698. The insert contains the CoMV promoter region, cp4epsps, nptII, aad (that is not expressed in plants) and a portion of ori-V coding regions. The cp4epsps gene encodes the naturally glyphosate tolerant EPSPS protein, the target site for glyphosate action. The nptII gene which is isolated from the Tn5 transposon encodes neomycin phosphotranferase type II (NPTII) which confers resistance to the aminoglycoside antibiotic kanamycin and neomycin. The nptII gene functions as a selectable marker and is driven by Cauliflower Mosaic Virus 35S promoter and is followed by a nopaline synthase (nos) 3' region which directs polyadenylation of the messenger RNA (mRNA). The aad gene encodes the bacterial selectable marker enzyme 3"(9) -O-aminoglycoside adenyltransferase (AAD) which allows for the selection of the bacteria containing the PV-GHGT06 to grow on medium with spectinomycin or streptomycin. This aad gene is not expressed in Roundup Ready 1698.
Vector information
The plasmid vector PV-GHGT06 contains well characterized DNA segments required for selection and replication of the plasmid in the bacteria as well as a right border for initiating the region of T-DNA, into the plant genomic DNA. The plant expression vectors were assembled, transformed in E. coli and mated into the ABI Agrobacterium strain by the triparental conjugation system using the helper plasmid pRK2013. The binary ABI strain contains the disarmed (lacking the T-DNA phytohormone genes) pTiC58 plasmid pMP90RK in a choramphenicol-resistant derivative of the A. tumefaciens strain A208. The disarmed pMP90RK Ti plasmid does not carry the T-DNA phytohormone genes and is unable to grow the crown gall disease.
EN
Molecular characterization demonstrates that one T-DNA (transferred) insert was integrated into the cotton genome to produce cotton 1698. The insert contains the CoMV promoter region, cp4epsps, nptII, aad (that is not expressed in plants) and a portion of ori-V coding regions. The cp4epsps gene encodes the naturally glyphosate tolerant EPSPS protein, the target site for glyphosate action. The nptII gene which is isolated from the Tn5 transposon encodes neomycin phosphotranferase type II (NPTII) which confers resistance to the aminoglycoside antibiotic kanamycin and neomycin. The nptII gene functions as a selectable marker and is driven by Cauliflower Mosaic Virus 35S promoter and is followed by a nopaline synthase (nos) 3' region which directs polyadenylation of the messenger RNA (mRNA). The aad gene encodes the bacterial selectable marker enzyme 3"(9) -O-aminoglycoside adenyltransferase (AAD) which allows for the selection of the bacteria containing the PV-GHGT06 to grow on medium with spectinomycin or streptomycin. This aad gene is not expressed in Roundup Ready 1698.
Vector information
The plasmid vector PV-GHGT06 contains well characterized DNA segments required for selection and replication of the plasmid in the bacteria as well as a right border for initiating the region of T-DNA, into the plant genomic DNA. The plant expression vectors were assembled, transformed in E. coli and mated into the ABI Agrobacterium strain by the triparental conjugation system using the helper plasmid pRK2013. The binary ABI strain contains the disarmed (lacking the T-DNA phytohormone genes) pTiC58 plasmid pMP90RK in a choramphenicol-resistant derivative of the A. tumefaciens strain A208. The disarmed pMP90RK Ti plasmid does not carry the T-DNA phytohormone genes and is unable to grow the crown gall disease.
EN
- Food
- Feed
- Fiber/textile
Southern blot analysis indicated that there is a single locus into which the T-DNA integrated and that DNA contain 2 copies of the EPSPS coding sequence
EN
Cotton line 1698 was genetically engineered to express resistance to glyphosate, the active ingredient in the herbicide Roundup®, allowing for its use as a weed control option. In order to obtain field tolerance to glyphosate herbicide, a bacterial gene encoding a glyphosate-tolerant form of the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) was introduced into the cotton genome by Agrobacterium-mediated transformation.
The EPSPS enzyme is present in all plants, bacteria, and fungi, and is part of an important biochemical pathway called the shikimate pathway, which is involved in the production of aromatic amino acids and other aromatic compounds. When conventional cotton plants are treated with glyphosate, the plants cannot produce the aromatic amino acids needed to grow and survive. EPSPS is not present in mammals, birds or aquatic life forms, which do not synthesize their own aromatic amino acids. For this reason, glyphosate has little toxicity to these organisms. The EPSPS enzyme is naturally present in foods derived from plant and microbial sources.
EN
The EPSPS enzyme is present in all plants, bacteria, and fungi, and is part of an important biochemical pathway called the shikimate pathway, which is involved in the production of aromatic amino acids and other aromatic compounds. When conventional cotton plants are treated with glyphosate, the plants cannot produce the aromatic amino acids needed to grow and survive. EPSPS is not present in mammals, birds or aquatic life forms, which do not synthesize their own aromatic amino acids. For this reason, glyphosate has little toxicity to these organisms. The EPSPS enzyme is naturally present in foods derived from plant and microbial sources.
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