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Modified Organism
BXN-1Ø222-2 - BXN™ cotton
Record information and status
Record ID
14825
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2014-04-08 21:49 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-04-08 21:49 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
BXN™ cotton
Transformation event
10222
Unique identifier
BXN-1Ø222-2
Developer(s)
Description
Cotton tolerant to oxynil herbicides, through introduction of the bxn gene isolated from the bacterium Klebsiella pneumoniae subspecies ozaenae which codes for the enzyme nitrilase, which hydrolyses ioxynil and bromoxynil into non-toxic compounds.  The aphII gene was isolated from the bacterium Eschericia coli confers tolerance to the antibiotic kanamycin (used as a selectable marker).
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Gossypium hirsutum - Cotton
Point of collection or acquisition of the recipient organism
Line: Coker 315
Related LMOs
BXN-1Ø215-4 - BXN™ cotton
Resistance to antibiotics - Kanamycin Resistance to herbicides - Bromoxynil Selectable marker genes and reporter genes
BXN-1Ø224-4 - BXN™ cotton
Resistance to antibiotics - Kanamycin Resistance to herbicides - Bromoxynil Selectable marker genes and reporter genes
BXN-1Ø211-9 - BXN™ cotton
Resistance to antibiotics - Kanamycin Resistance to herbicides - Bromoxynil Selectable marker genes and reporter genes
Characteristics of the transformation process
Vector
pBrx75
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Ti plasmid left border repeat
0.00 Kb
 
 
Tumour Morphology Large gene terminator
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Aminoglycoside 3 phosphotransferase II
0.00 Kb
 
 
CaMV 35S promoter
0.00 Kb
 
 
CaMV 35S promoter
0.00 Kb
 
 
Bromoxynil-specific nitrilase
0.00 Kb
 
 
Tumour Morphology Large gene terminator
0.00 Kb
 
 
Ti plasmid right border repeat
0.00 Kb
 
Further details
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Fiber / Textile
Additional Information
Additional Information
The BXN™ cotton line was genetically engineered to express tolerance to oxynil herbicides, including bromoxynil and ioxynil. Oxynil herbicides act by blocking electron flow during the light reaction of photosynthesis, inhibiting cellular respiration in dicotyledonous plants. Oxynil herbicides applied at rates recommended for effective weed control are toxic to conventional cotton varieties. The modified cotton line BXN™ contains the bxn gene for oxynil tolerance, and allows farmers to use oxynil herbicides for weed control in the cultivation of cotton.

The bxn gene was isolated from the bacterium Klebsiella pneumoniae subspecies ozaenae and codes for the enzyme nitrilase, which hydrolyses ioxynil and bromoxynil into non-toxic compounds. The nitrilase encoding gene was introduced into the cotton genome using Agrobacterium-mediated transformation, and the bacterial form of the enzyme expressed in this transgenic cotton line functions the same as that found in monocot plants such as corn, wheat and barley.

The kanamycin-resistance gene (aphII), isolated from the bacterium Escherichia coli codes for an enzyme (aminoglycoside 3'-phosphotransferase II) that phosphorylates kanamycin , thereby preventing it from binding to ribosomes and rendering the cells resistant. This protein is ubiquitous in the environment and it degrades rapidly in vitro in simulated mammalian gastric and intestinal fluids.

Records referencing this document (7)
IDDescription
7record(s) found
Country's Decision or any other Communication3 records
Modified Organism3 records
Risk Assessment1 record