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Modified Organism
FLO-4Ø619-8 - Moonshade™ carnation
Record information and status
Record ID
14828
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2020-11-23 15:03 UTC (austein.mcloughlin@cbd.int)
Date of publication
2020-11-23 15:03 UTC (austein.mcloughlin@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Moonshade™ carnation
Transformation event
123.2.2 (40619)
Unique identifier
FLO-4Ø619-8
Developer(s)
Stephen Chandler
Cosnultant
SUntory Holdings Ltd.
Melbourne, VIC
Australia
Phone:+61 409 387 386
Email:schandler@florigene.com.au
Url:Florigene Homepage
Description
Carnations with modified colour and sulfonylurea herbicide tolerance, produced by inserting two anthocyanin biosynthetic genes from Petunia hybrida, dihydroflavonol reductase (dfr) and Hf1 encoding flavonoid 3',5'hydroxylase (F3'5'H), whose expression results in a violet/mauve colouration. Tolerance to sulfonyl urea herbicides was produced through the introduction of a chlorsulfuron tolerant version of the acetolactate synthase (ALS) encoding gene from Nicotiana tabacum.

NOTE: This LMO was formerly referred to with the UID FLO-4Ø619-7.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Dianthus caryophyllus - Carnation, DIACA
Point of collection or acquisition of the recipient organism
A white carnation cultivar FE 123, belonging to Dianthus caryophyllus L., Dianthus, Caryophyllaceae, was used as the recipient organism.
Related LMOs
FLO-Ø7442-5 - Moondust™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
FLO-4Ø644-6 - Moonlite™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
FLO-ØØØ15-3 - Moondust™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
FLO-ØØØ16-4 - Moondust™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
FLO-ØØØØ4-1 - Moondust™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
FLO-4Ø62Ø-9 - Moonburst™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
Characteristics of the transformation process
Vector
pCGP1470
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.19 Kb
 
 
5' untranslated leader of chlorophyll a/b-binding protein
0.06 Kb
 
 
Acetohydroxy acid synthase gene
3.76 Kb
 
 
Acetohydroxy acid synthase gene terminator
0.00 Kb
 
 
Chalcone synthase gene promoter
1.16 Kb
 
 
Flavonoid 3', 5' hydroxylase gene
1.52 Kb
 
 
D8 gene terminator
0.82 Kb
 
 
mac-1 Promoter
1.23 Kb
 
 
Dihydroflavonol-4-reductase
1.14 Kb
 
 
Mannopine synthase gene terminator
0.72 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Gene expression
The T-DNA insertion contained three gene cassettes: Nicotiania tabacum acetolactate synthase (ALS; acetohydroxy acid synthase), Petunia hybrida flavonoid 3', 5' hydroxylase (F3'5'H) and P. hybrida dihydroflavonol-4-reductase (DFR).

Transcription of ALS is under control of the Cauliflower mosaic virus (CaMV) 35S promoter and the ALS terminator. A 5' untranslated leader from P. hybrida chlorophyll a/b-binding protein is also transcribed, but is not expected to be translated. The leader sequence augments the level of transcription (enhances gene expression) of ALS.

Transcription of F3'5'H is under control of the Antirrhinum majus chalcone synthase gene promoter and the P. hybrida D8 terminator.

Transcription of DFR is under control of the synthetic Mac-1 promoter and the Agrobacterium tumefaciens mannopine synthase gene terminator.


Note:
- The genetic element size of the ALS coding sequence includes the size of the terminator (3.76 kb = size of coding sequence + terminator)
- The Mac-1 promoter is a synthetic promoter compromised of A. tumefaciensmas promoter and CaMV enhancer sequences.
LMO characteristics
Modified traits
  • Selectable marker genes and reporter genes
Common use(s)
  • Ornamental
Additional Information
Additional Information
This transgenic carnation was developed using recombinant DNA techniques to produce flowers with a unique violet/mauve colour by introducing two genes from petunia (Petunia hybrida) that function together in the biosynthesis of the anthocyanin pigment delphinidin. The transgenic lines were derived from the parent cultivar 'White Unesco', which is a white coloured carnation that was selected for a mutation in the dihydroflavonol reductase (DFR) encoding gene that did not allow for expression of a functional enzyme, and thus did not produce the anthocyanin type pigments that give rise to blue and red coloured flowers. The two genes introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene and a gene encoding the enzyme flavonoid 3', 5'-hydroxylase (F3', 5'H), a member of the NADPH-Cytochrome P450 reductase family. Expression of these genes allows for the production of blue coloured delphinidin anthocyanin pigments, which are not normally found in carnations.
Other relevant website address or attached documents

Records referencing this document (14)
IDDescription
14record(s) found
Country's Decision or any other Communication4 records
Modified Organism6 records
Risk Assessment4 records