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Modified Organism
FLO-114ØØ-3 - Moonshade™ carnation
Record information and status
Record ID
14833
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2015-10-15 17:03 UTC (dina.abdelhakim@cbd.int)
Date of publication
2015-10-15 17:03 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Moonshade™ carnation
Transformation event
1400A (11400)
Unique identifier
FLO-114ØØ-3
Developer(s)
Stephen Chandler
Cosnultant
SUntory Holdings Ltd.
Melbourne, VIC
Australia
Phone:+61 409 387 386
Email:schandler@florigene.com.au
Url:Florigene Homepage
Description
Carnation with a modified flower colour, a shade of light mauve, whereas the non-GM parent has cream-white flowers. The colour has been achieved by introducing into white carnation two genes of the anthocyanin biosynthesis pathway from Petunia and Viola sp. These genes, encoding dihydroflavonol 4-reductase (dfr) and flavonoid 3'5' hydroxylase (f3'5'h), together with other genes of the anthocyanin biosynthesis pathway already present in the non GM carnation, give rise to the anthocyanins delphinidin and cyanidin.

Note: This line was never commercialised

NOTE: This LMO was formerly referred to with the UID FLO-114ØØ-2.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Dianthus caryophyllus - Carnation, DIACA
Related LMOs
FLO-11363-2 - Moonshadow™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
FLO-11226-9 - Moonshade™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
FLO-4Ø689-6 - Moonaqua™ carnation
Dr Yoshikazu Tanaka Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
FLO-4Ø685-2 - Moonvista™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
FLO-11351-8 - Moonshade™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
FLO-11959-4 - Moonshade™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
FLO-11988-6 - Moonshade™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
Characteristics of the transformation process
Vector
pCGP1991
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.20 Kb
 
 
5' untranslated leader of chlorophyll a/b-binding protein
0.06 Kb
 
 
Acetohydroxy acid synthase gene
3.77 Kb
 
 
Acetohydroxy acid synthase gene terminator
0.00 Kb
 
 
Dihydroflavonol-4-reductase promoter
0.00 Kb
 
 
Dihydroflavonol-4-reductase
4.96 Kb
 
 
Dihydroflavonol-4-reductase terminator
0.00 Kb
 
 
Chalcone synthase gene promoter
1.17 Kb
 
 
Flavonoid 3’, 5’-hydroxylase gene
1.80 Kb
 
 
D8 gene terminator
0.81 Kb
 
Further details
LMO characteristics
Modified traits
Common use(s)
  • Ornamental
Additional Information
Additional Information
Transgenic line 1400A was developed using recombinant DNA techniques to produce flowers with a unique deep purple colour by introducing two genes that function together in the biosynthesis of the anthocyanin pigment delphinidin. The transgenic line was derived from the parent cultivar 'White Unesco', which is a white coloured carnation that was selected for a mutation in the dihydroflavonol reductase (DFR) encoding gene that did not allow for expression of a functional enzyme, and thus did not produce the anthocyanin type pigments that give rise to blue and red coloured flowers.

The two genes introduced into the transgenic carnation line included a functional dihydroflavonol reductase encoding gene isolated from petunia (Petunia hybrida) and a gene encoding the enzyme flavonoid 3', 5'-hydroxylase (F3', 5'H), a member of the NADPH-Cytochrome P450 reductase family, isolated from Viola. Expression of the F3', 5'H encoding gene (bp40) allows for the production of blue coloured delphinidin anthocyanin pigments, which are not normally found in carnations.

Tolerance to sulfonyl urea herbicides was produced via the introduction of a chlorsulfuron tolerant version of the acetolactate synthase (ALS) encoding gene from tobacco.
Other relevant website address or attached documents

Records referencing this document (7)
IDDescription
7record(s) found
Modified Organism7 records