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Modified Organism
FLO-4Ø685-2 - Moonvista™ carnation
Record information and status
Record ID
14835
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2015-01-26 19:13 UTC (dina.abdelhakim@cbd.int)
Date of publication
2015-01-26 19:13 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Moonvista™ carnation
Transformation event
123.8.8 (40685)
Unique identifier
FLO-4Ø685-2
Developer(s)
Stephen Chandler
Cosnultant
SUntory Holdings Ltd.
Melbourne, VIC
Australia
Phone:+61 409 387 386
Email:schandler@florigene.com.au
Url:Florigene Homepage
Description
Moonvista™ is a carnation variety in which the flowers have a modified colour as the result of genes enabling the biosynthesis of delphinidin pigment. The flowers also carry an herbicide resistance gene to facilitate selection in vitro.

NOTE: This LMO was formerly referred to with the UID FLO-4Ø685-1.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Dianthus caryophyllus - Carnation, DIACA
Related LMOs
FLO-11363-2 - Moonshadow™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
FLO-11226-9 - Moonshade™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
FLO-4Ø689-6 - Moonaqua™ carnation
Dr Yoshikazu Tanaka Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
FLO-11351-8 - Moonshade™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
FLO-114ØØ-3 - Moonshade™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
FLO-11959-4 - Moonshade™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
FLO-11988-6 - Moonshade™ carnation
Stephen Chandler Changes in quality and/or metabolite content - Pigmentation / Coloration Resistance to herbicides - Chlorsulfuron, Sulfonylurea
Characteristics of the transformation process
Vector
pCGP1991
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.19 Kb
 
 
5' untranslated leader of chlorophyll a/b-binding protein
0.08 Kb
 
 
Acetohydroxy acid synthase gene
3.76 Kb
 
 
Acetohydroxy acid synthase gene terminator
0.00 Kb
 
 
Chalcone synthase gene promoter
1.16 Kb
 
 
Flavonoid 3’, 5’-hydroxylase gene
1.79 Kb
 
 
D8 gene terminator
0.82 Kb
 
 
Dihydroflavonol-4-reductase promoter
0.00 Kb
 
 
Dihydroflavonol-4-reductase
4.96 Kb
 
 
Dihydroflavonol-4-reductase terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Colour-modified carnation produced through introduction of two anthocyanin biosynthetic genes to result in a violet/mauve colouration, the dfr gene which encodes dihydroflavonol reductase and the Hf1 gene which encodes flavonoid 3',5'hydroxylase (F3'5'H) from Petunia hybrida.  A variant form of acetolactate synthase (ALS) from Nicotiana tabacum was used as a selectable marker to confer tolerance to sulfonylurea herbicide.

There are up to five copies of each integrated component of the T-DNA, and integration of T-DNA has occurred at four loci. Estimated copy numbers of T-DNA components integrated in transgenic line FLO-40685-1 as follows: LB - 2 copies, NtALS (SurB) - 2 copies, VhF3'5'H - 4 copies, PhDFR - 2 copies, RB - 5 copies.
LMO characteristics
Modified traits
Common use(s)
  • Ornamental
Additional Information
Additional Information
These carnations were developed using recombinant DNA techniques to produce flowers with a unique violet/mauve colour by introducing two genes from petunia (Petunia hybrida) that function together in the biosynthesis of the anthocyanin pigment delphinidin. The transgenic lines were derived from the parent cultivar 'White Unesco', which is a white coloured carnation that was selected for a mutation in the dihydroflavonol reductase (DFR) encoding gene that did not allow for expression of a functional enzyme, and thus did not produce the anthocyanin type pigments that give rise to blue and red coloured flowers. The two genes from Petunia hybrida introduced into the transgenic carnation lines included a functional dihydroflavonol reductase encoding gene (dfr) and a gene (hf1) encoding the enzyme flavonoid 3', 5'-hydroxylase (F3'5'H), a member of the NADPH-Cytochrome P450 reductase family. Expression of the F3'5'H encoding gene allows for the production of blue coloured delphinidin anthocyanin pigments, which are not normally found in carnations.

Tolerance to sulfonyl urea herbicides was produced via the introduction of a chlorsulfuron tolerant version of the acetolactate synthase (ALS) encoding gene from tobacco (SuRB).
Other relevant website address or attached documents

Records referencing this document (16)
IDDescription
16record(s) found
Country's Decision or any other Communication4 records
Modified Organism8 records
Risk Assessment4 records