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Modified Organism
FLO-ØØØ66-9 - Long vase life carnation
Record information and status
Record ID
14839
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2015-01-26 22:52 UTC (dina.abdelhakim@cbd.int)
Date of publication
2015-01-26 22:52 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Long vase life carnation
Transformation event
66
Unique identifier
FLO-ØØØ66-9
Developer(s)
Stephen Chandler
Cosnultant
SUntory Holdings Ltd.
Melbourne, VIC
Australia
Phone:+61 409 387 386
Email:schandler@florigene.com.au
Url:Florigene Homepage
Description
Long vase life (delayed senescence) carnations produced by inserting a truncated copy of the carnation 1-amino-cyclopropane-1-carboxylic acid (ACC) synthase encoding gene in order to suppress expression of the endogenous unmodified gene, which is required for normal ethylene biosynthesis. Tolerance to sulfonyl urea herbicides was via the introduction of a chlorsulfuron tolerant version of the acetolactate synthase (ALS) encoding gene from tobacco.

NOTE: This LMO was formerly referred to with the UID FLO-ØØØ66-8.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Dianthus caryophyllus - Carnation, DIACA
Point of collection or acquisition of the recipient organism
Cultivar: Ashley
Characteristics of the transformation process
Vector
pWTT2160
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.00 Kb
 
 
1-amino-cyclopropane -1-carboxylic acid synthase gene
0.00 Kb
 
 
Nopaline Synthase Gene Terminator
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CaMV 35S promoter
0.00 Kb
 
 
Acetohydroxy acid synthase gene
0.00 Kb
 
 
Acetohydroxy acid synthase gene terminator
0.00 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The enzyme 1-aminocyclopropane-1-carboxyllic acid (ACC) synthase, normally found in carnations, is responsible for the conversion of s-adenosylmethionine to ACC, which is the immediate precursor of ethylene. The transgenic carnation line 66 was developed using recombinant DNA techniques to display suppressed ACC synthase activity, and thus reduced ethylene synthesis and therefore longer vase life, by inserting an additional sense orientation, truncated copy of the ACC synthase encoding gene. The presence of the truncated ACC synthase gene suppresses the normal expression of the native ACC synthase gene, and while not completely understood, the mechanism of "downregulation" is likely linked to the coordinate suppression of transcription of both the endogenous gene and the introduced truncated ACC synthase gene.
LMO characteristics
Modified traits
Common use(s)
  • Ornamental
Additional Information
Other relevant website address or attached documents

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