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Modified Organism
DAS-Ø15Ø7-1 - Herculex™ I maize
Record information and status
Record ID
14841
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-04-08 19:48 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-04-08 19:48 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Herculex™ I maize
Transformation event
TC1507
Unique identifier
DAS-Ø15Ø7-1
Developer(s)
Pioneer Hi-Bred International Inc.
Url:Pioneer HiBred International Homepage
Description
Corm modified with the insertion of the Cry1F gene from Bacillus thuringiensis to confer resistance to the European corn borer (Ostrinia nubilalis). A transformation cassette coding for phosphinothricin (PPT) herbicide tolerance, specifically glufosinate ammonium, was also inserted into the organism.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Characteristics of the transformation process
Vector
PHI8999A derived from plasmid PHP8999
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
Ubiquitin gene promoter
1.98 Kb
 
 
Cry1F
1.82 Kb
 
 
ORF25 PolyA Terminator sequence
0.72 Kb
 
 
CaMV 35S promoter
0.55 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
CaMV 35S terminator
0.20 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
Notes about the Other(s) sequence(s) specific to this LMO

DNA fragment PHI8999A contains two adjacent plant gene expression cassettes for the cry1F and pat genes (see below). Transcription of the cry1F gene was directed by the promoter and first exon and intron of the maize ubiquitin gene. The 3' terminator sequence used was the 3' polyadenylation signal from ORF25 (Agrobacterium tumefaciens) (see footnote*). Transcriptional regulation of the pat gene from Streptomyces viridochromogenes was via promoter and terminator sequences derived from the 35S transcript of cauliflower mosaic virus (CaMV). The coding sequence of both genes has been optimised to achieve a high level of expression in maize.

The sequences of the complete cry1F and pat genes are identical to those in the original plasmid. The proteins produced in the modified plants are the ones intended, including a leucine residue (replacing a phenylalanine) at position 604 (of 605 amino acids in total) of Cry1F. This modification was introduced to create a specific restriction site for cloning purposes.

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Footnote *:  The EFSA document (see below) indicates that the 3' sequence from the Agrobacterium tumefaciens mannopine synthase gene was used as terminator of the cry1F gene.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Biofuel
Detection method(s)
Additional information
Molecular analyses of the transformed plant show that the event TC1507 contains one site of integration of the introduced DNA which includes a full-length of the DNA fragment used for transformation (i.e. the ~6235 bp of DNA cassette containing the cry1F and pat genes) and an additional copy of the cry1F gene lacking the majority of the associated ubiquitin regulatory sequences.

Southern analysis using a cry1F probe carried out by the European Food Safety Authority (EFSA; see document below) revealed the presence of two cry1F inserts. The first represented the intact gene from the expression cassette. The second insert was a truncated cry1F  fragment of 335bp, which is located at the 5' end of the insertion locus. In addition, analysis of the sequences adjacent to the insert of fragment PHI8999A revealed DNA fragments that correspond to small segments from PHI8999A, including incomplete sequences from the pat gene, the maize ubiquitin promoter and the terminator from Agrobacterium. Furthermore, different fragments of chloroplast DNA and a number of sequences with similarity to retrotransposons are also present in the border region of the insert.

Records referencing this document (189)
IDDescription
189record(s) found
Country's Decision or any other Communication65 records
Information Resource1 record
Modified Organism29 records
Organization23 records
Risk Assessment71 records