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Modified Organism
ACS-GMØØ4-2 - Herbicide-tolerant soybean
Record information and status
Record ID
14855
Status
Published
Date of creation
2006-06-05 14:40 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-01-18 21:15 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-01-18 21:15 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Herbicide-tolerant soybean
Transformation event
A2704-21
Unique identifier
ACS-GMØØ4-2
Developer(s)
Description
The soybean line A2704-12 was developed to allow for the use of glufosinate ammonium, the active ingredient in phosphinothricin herbicides (Basta® , Ignite® , Rely® , Liberty® , Harvest® , and Finale®) as a weed control option. These genetically engineered soybean lines contain the fungal enzyme phosphinothricin-N-acetyltransferase (PAT) that allows these plants to survive the otherwise lethal application of glufosinate. The pat gene inserted was isolated from a common soil fungus, Streptomyces viridochromogenes, and introduced into the soybean genome by particle acceleration (biolistic) transformation.

The PAT enzyme converts L-phosphinothricin (PPT), the active ingredient in glufosinate ammonium, to an inactive form thereby conferring resistance to the herbicide. In absence of PAT, application of glufosinate leads to reduced production of the amino acid glutamine and increased ammonia levels in the plant tissues which are lethal to the plant. The PAT enzyme is not known to have any toxic properties.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Glycine max - Soybean, Soya bean, Soya, SOYBN
Related LMOs
ACS-GMØØ5-3 - Herbicide-tolerant soybean
Resistance to herbicides - Glufosinate
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ACS-GMØØ1-8 - Herbicide-tolerant soybean
Resistance to antibiotics - Ampicillin Resistance to herbicides - Glufosinate Selectable marker genes and reporter genes
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ACS-GMØØ2-9 - Herbicide-tolerant soybean
Resistance to antibiotics - Ampicillin Resistance to herbicides - Glufosinate Selectable marker genes and reporter genes
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ACS-GMØØ3-1 - Herbicide-tolerant soybean
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ACS-GMØØ6-4 - Liberty Link™ soybean
Resistance to herbicides - Glufosinate
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ACS-GMØØ8-6 - Herbicide-tolerant soybean
Resistance to herbicides - Glufosinate
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Characteristics of the transformation process
Vector
pB2/35SAcK (AKA: pWRG5143)
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
CaMV 35S promoter
0.54 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
CaMV 35S terminator
0.20 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The PAT gene from Streptomyces viridochromogenes was synthetically modified with plant preferred codons.

The bacterial ampicillin resistance gene was also integrated into the host genome. It is however only expressed in bacterial cells.
LMO characteristics
Modified traits
Common use(s)
  • Food
Detection method(s)
Additional information
Southern blot analysis indicated that 5 copies of the PAT gene and 2 copies of the ampR gene were integrated into the host genome.
Additional Information
Other relevant website address or attached documents

Records referencing this document (11)
IDDescription
11record(s) found
Country's Decision or any other Communication2 records
Modified Organism6 records
Organization3 records