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Modified Organism
ACS-ZMØØ4-3 - Starlink™ maize
Record information and status
Record ID
14862
Status
Published
Date of creation
2006-06-05 14:40 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-04-18 20:24 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-04-18 20:24 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Starlink™ maize
Transformation event
CBH-351
Unique identifier
ACS-ZMØØ4-3
Developer(s)
Description
The transgenic Starlink™ maize line CBH-351 was developed to for insect resistance and glufosinate herbicide tolerance. Resistance to lepidopteran pests was confereed through the insertion of the Cry9C coding sequence. Tolerance to glufosinate is conferred through the insertion of the coding sequence for phosphinothricin N-acetyltransferase (PAT).
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Characteristics of the transformation process
Vector
pRVA9909 and pDE110
Techniques used for the modification
  • Biolistic / Particle gun
Genetic elements construct
 
CaMV 35S promoter
0.53 Kb
 
 
5' untranslated leader of chlorophyll a/b-binding protein
0.06 Kb
 
 
Cry9C
1.88 Kb
 
 
CaMV 35S terminator
0.21 Kb
 
 
CaMV 35S promoter
1.38 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The recipient organism was co-transformed with the pRVA9909 and pDE110 vectors. The pRVA9909 vector contained the transformation cassette for Cry9C and the pDE110 vector contained the transformation cassette for the bar gene.

Southern blot analysis indicated that the LMO contains at least one intact copy of the Cry9c expression cassette and at least 4 copies of the PAT expression cassette one of which integrated into the genome without its regulatory elements.
The test also confirmed that there was integration of at least 4 copies of the bacterial β-lactamse gene. However this is non-functional in plant tissues.

The Cry9c DNA sequence was modified to truncate the C-terminal amino acids following amino acid position 666 and the first 43 amino acids at the N-terminal. Additionally the argenine amino acid at position 123 was modified to a lysine. These modifications do not affect the insecticidal activity of the protein.
LMO characteristics
Modified traits
Common use(s)
  • Feed
Additional Information
Other relevant website address or attached documents

Records referencing this document (5)
IDDescription
5record(s) found
Country's Decision or any other Communication1 record
Organization4 records