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Modified Organism
DAS-59122-7 - Herculex™ RW Rootworm Protection maize
Record information and status
Record ID
15165
Status
Published
Date of creation
2006-06-20 10:10 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2014-01-15 17:20 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-01-15 17:20 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Herculex™ RW Rootworm Protection maize
Transformation event
59122
Unique identifier
DAS-59122-7
Developer(s)
Pioneer Hi-Bred International Inc.
Url:Pioneer HiBred International Homepage
Description
The transgenic maize line DAS-59122-7 was genetically modified to resist Coleopteran insects (e.g. western corn rootworm, northern corn rootworm and Mexican corn rootworm) and to tolerate herbicides containing glufosinate ammonium.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Zea mays - Maize, Corn, MAIZE
Related LMOs
DAS-59132-8 - Insect resistant Herbicide tolerant Maize
Resistance to diseases and pests - Insects - Coleoptera (beetles) Resistance to herbicides - Glufosinate
Show detection method(s)
Characteristics of the transformation process
Vector
PHP17662
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Ubiquitin gene promoter
1.99 Kb
 
 
Cry34Ab1
0.37 Kb
 
 
Proteinase inhibitor II gene terminator
0.32 Kb
 
 
Peroxidase gene promoter
1.30 Kb
 
 
Cry35Ab1
1.15 Kb
 
 
Proteinase inhibitor II gene terminator
0.32 Kb
 
 
CaMV 35S promoter
0.55 Kb
 
 
Phosphinothricin N-acetyltransferase gene
0.55 Kb
 
 
CaMV 35S terminator
0.20 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The cry34Ab1 and cry35Ab1 were cloned from Bacillus thuringiensis strain PS149B1 and the coding sequence of both genes has been adapted to the codon usage in maize as to achieve expression in the maize plant.

Sequence analysis of 59122 done by the European Food Safety Authority indicated that this LMO contains one complete copy of the T-DNA of PHP17662 without internal rearrangements. All three gene cassettes, cry34Ab1, cry35Ab1 and pat, are intact within the transgenic event. The DNA sequences of the genes in 59122 are identical to those in the original plasmid except for two nucleotide differences in the wheat peroxidise promoter. At the 5' T-DNA end a deletion of 22 bp is observed and at the 3' T-DNA end a deletion of 25 bp is observed. The absence of vector backbone in maize 59122 was also demonstrated.
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
  • Biofuel
Additional Information

Records referencing this document (126)
IDDescription
126record(s) found
Country's Decision or any other Communication32 records
Information Resource1 record
Modified Organism44 records
Organization18 records
Risk Assessment31 records