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Modified Organism
Tomato modified for delayed ripening
Record information and status
Record ID
15395
Status
Published
Date of creation
2006-07-13 14:16 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2014-04-08 19:22 UTC (dina.abdelhakim@cbd.int)
Date of publication
2014-04-08 19:22 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Tomato modified for delayed ripening
Transformation event
1345-4
Developer(s)
DNA Plant Technology Corporation
Description
Delayed ripening tomatoes, created through introduction of a gene sequence encoding the enzyme 1-amino-cyclopropane-1-carboxylic acid synthase (ACC) that metabolises the precursor of the fruit ripening hormone ethylene. The neomycin phosphotransferase II (npt II) confers resistance to the antibiotic kanamycin.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum lycopersicum - Tomato, SOLLC
Point of collection or acquisition of the recipient organism
Cultivar: 91103-114
Characteristics of the transformation process
Vector
pWTT2144/AccS
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Nopaline Synthase Gene Promoter
0.60 Kb
 
 
Neomycin Phosphotransferase II
1.02 Kb
 
 
Octopine Synthase Gene Terminator
0.56 Kb
 
 
CaMV 35S promoter
0.96 Kb
 
 
5' untranslated leader from chlorophyll a/b-binding protein
0.07 Kb
 
 
1-amino-cyclopropane -1-carboxylic acid synthase gene
1.09 Kb
 
 
Nopaline Synthase Gene Terminator
0.27 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
A 1088bp truncated segment of the Acc2 gene (designated AccS) was inserted into the host genome. The genetic element does not result in a functional protein and the expression of the AccS gene results in the sense suppression of the endogenous gene. The nos promoter is present as a duplicate tandem repeat of the 5' untranslated region of the nopaline synthase gene.

Southern blot analysis indicated that three copies of the T-DNA inserted at a single locus within the host genome in an inverted fashion. There was no incorporation of translatable plasmid DNA sequences outside of the T-DNA region.
LMO characteristics
Modified traits
Common use(s)
  • Food
Additional Information
Additional Information
The 1345-4 line of tomato (Lycopersicon esculentum) was developed through a specific genetic modification of cultivar 91103-114 to exhibit a decreased activity of the enzyme 1-aminocyclopropane-1- carboxylic acid (ACC) synthase. This endogenous enzyme is responsible for the conversion of s-adenosylmethionine to ACC, which is the immediate precursor of ethylene, a phytohormone known to play a key role in fruit ripening. The in situ accumulation of ethylene in the transgenic tomatoes is only about 1/50 the level found in the unmodified parental line and the fruit does not fully ripen unless an external source of ethylene is applied.

While not completely understood, the mechanism of "downregulation" of the endogenous ACC synthase gene is likely linked to the coordinate suppression of transcription of both the endogenous gene and the introduced truncated ACC synthase gene.

Records referencing this document (4)
IDDescription
4record(s) found
Country's Decision or any other Communication3 records
Risk Assessment1 record