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Modified Organism
Insect resistant tomato
Record information and status
Record ID
15396
Status
Published
Date of creation
2006-07-13 14:24 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-04-15 13:30 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-04-15 13:30 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Insect resistant tomato
Transformation event
5345
Developer(s)
Monsanto
800 North Lindbergh Blvd.
St. Louis, MO
United States of America, 63167
Phone:+ 1 314 694-1000
Fax:+1 314 694-3080
Url:Monsanto
Description
Tomato was modified for resistance against certain tomato feeding Lepidopteran insects via the insertion of a copy of the Cry1Ac gene.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Solanum lycopersicum - Tomato, SOLLC
Characteristics of the transformation process
Vector
PV-LEBK04
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
CaMV 35S promoter
0.32 Kb
 
 
Neomycin Phosphotransferase II
0.79 Kb
 
 
Nopaline Synthase Gene Terminator
0.26 Kb
 
 
CaMV Enhanced 35S promoter
0.62 Kb
 
 
Cry1Ac
3.50 Kb
 
 
α' subunit of β-conglycinin gene terminator
0.43 Kb
 
Further details
Notes regarding the genetic elements introduced or modified in this LMO
The Cry1Ac coding sequence was modified for plant optimised codons and resulted in a single amino acid change at L766S.

Southern blot analysis indicated that a single intact copy of the T-DNA was inserted into the host genome
LMO characteristics
Modified traits
Common use(s)
  • Food
Additional Information
Additional Information
Insect-resistant tomato line 5345 was developed using recombinant DNA techniques to express the insecticidal protein, Cry1Ac, encoded by the cry1Ac gene from the soil bacterium Bacillus thuringiensis subsp. kurstaki strain HD73.

Insecticidal activity is caused by the selective binding of Cry1Ac protein to specific sites localized on the brush border midgut epithelium of susceptible lepidopteran species. Following binding, cation-specific pores are formed that disrupt midgut ion flow thereby causing gut paralysis and eventual death from bacterial sepsis.

Delta-endotoxins, such as the Cry1Ac protein expressed in tomato line 5345, exhibit highly selective insecticidal activity against a narrow range of lepidopteran pests. The specificity of action is directly attributable to the presence of specific receptors in the target insects. There are no receptors for delta-endotoxins of B. thuringiensis on the surface of mammalian intestinal cells, therefore, livestock animals and humans are not susceptible to these proteins.
Other relevant website address or attached documents

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