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Modified Organism
Rice containing cedar pollen peptide
Record information and status
Record ID
46122
Status
Published
Date of creation
2008-08-01 16:23 UTC (mia.crager@cbd.int)
Date of last update
2012-08-28 16:10 UTC (dina.abdelhakim@cbd.int)
Date of publication
2012-08-28 16:10 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
Rice containing cedar pollen peptide
Transformation event
7Crp#10
Developer(s)
National Institute of Agrobiological Sciences (NIAS)
Url:National Institute of Agrobiological Sciences
Description
For the cedar pollen antigen proteins Cryj I and Cryj II which have been identified as the antigens causing cedar pollen allergy, the T cell epitope (12-19 amino acids), recognized by the cedar allergen specific T cells, has been investigated in detail. Then, based on the idea that if the T-cell epitopes of cedar allergen could be accumulated in the daily ingested rice, "the rice possibly offering the effects of mitigating or curing the Japanese cedar pollinosis by ingestion" could be developed based on the oral immune tolerance phenomenon, the epitope peptide accumulated rice was developed.

Nucleotide sequence to express the part of amino acid sequence contained in the antigen proteins Cryj I and Cryj II in the cedar pollens causing pollen allergy and recognized by the human cedar pollen antigen-specific T cell (hereinafter referred to as "human T cell epitope".

At a total of seven (7) sites, three (3) in Cryj I and four (4) in Cryj II, the human T-cell epitopes, composed of 12 to 19 amino acid residues in length respectively, have been identified. In order to express the artificial peptide (7Crp) composed of 96 amino acid residues by linking the seven-site epitopes (amino acid sequence), the artificial gene was synthesized in accordance with the amino acid sequence of T cell epitope. For the synthesis, the frequently used codon was selected among the gene cluster which encodes the major rice seed storage proteins.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Oryza sativa - Rice, ORYSA
Characteristics of the transformation process
Vector
pGTV-35S-HPT
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Glutelin gene promoter
2.30 Kb
 
 
Glutelin signal peptide
0.07 Kb
 
 
7Crp gene
0.29 Kb
 
 
KDEL ER retention signal
0.01 Kb
 
 
Glutelin terminator
0.65 Kb
 
 
CaMV 35S promoter
0.80 Kb
 
 
Hygromycin B phosphotransferase gene
1.10 Kb
 
 
Transcript 7 gene 3' untranslated region
0.00 Kb
 
Further details
LMO characteristics
Modified traits
Common use(s)
  • Food

Records referencing this document (1)
IDDescription
1record(s) found
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