In MIR162 maize, a variant of the native Bt Vip3Aa, named vip3Aa19, which has codon changes that result in a single  M129I amino acid substitution was inserted into the transformation cassette. During the transformation process an additional DNA mutation resulted in a K284Q amino acid substitution. This final form was designated the name Vip3Aa20.

The pNOV1300 vector region between the left and right border sequences, which included the vip3Aa19 and pmi gene expression cassettes, was inserted into the maize genome during transformation.

Southern blot analyses demonstrated that the T-DNA insert contains: i) single copies of a vip3Aa20 gene and a pmi gene; ii) two copies of the ZmUbiInt promoter; iii) one copy of the NOS terminator; and iv) no backbone sequences from transformation plasmid pNOV1300.