The Streptomyces viridochromogenes phosphinothricin N-acetyltransferase (PAT) gene is under the control of the Andropogon gerardii ubiquitin (Ubq) gene promoter and the Oryza sativa alpha-amylase/trypsin inhibitor terminator. The transcript includes the A. gerardii 5' untranslated leader sequence and an intron from Ubq before the PAT.

The Stenotrophomonas maltophilia Dicamba monooxygenase (DMO) gene is under control of the peanut chlorotic streak caulimovirus (PC1SV) Full-length transcipt (FLt) promoter and the Triticum aestivum (wheat) heat shock protein 17 terminator. The transcript produced contains the wheat chlorophyll a/b-binding 5' untranslated leader sequence (for improved gene expression), the Oryza sativa Actin 1 untranslated region (UTR) and intron (for improved gene expression), the targeting and UTR of Petunia hybrida Chloroplast Transit Peptide 4, and DMO.

Note:
- Originally, the plasmid vector contained two T-DNA elements that were inserted during the initial transformation event: one containing the dmo and pat expression cassettes, and a second containing a cp4 epsps expression cassette. The cp4 epsps expression cassette is regulated by the Ract1 promoter from Oryza sativa, the Ract1 5′ untranslated leader from Oryza sativa, the Ract1 intron from Oryza sativa, the CTP2 targeting sequence from Arabidopsis thaliana, and the nos 3′ untranslated region from Agrobacterium tumefaciens. Subsequent traditional breeding, segregation, selection, and screening were used to isolate those plants that contain the dmo and pat expression cassettes (T-DNA I) and do not contain the cp4 epsps expression cassette (T-DNA II).
- Molecular characterization of MON 87419 indicated that a single copy of T-DNA I was integrated into the maize genome at a single intact locus that includes all expected elements within the insert, with the exception of incomplete Right and Left Border sequences. These analyses also showed no PV-ZMHT507801 backbone elements or T-DNA II sequences were present in the event.