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Organisme vivant modifié
(LMO)
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Herbicide-tolerant cotton
EN
GHB811
Oui
BCS-GH811-4
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Organisation :Bayer CropScience ()Téléphone :Télécopieur :E-mail :Site Web :
Cotton (Gossypium hirsutum) was modified for tolerance to glyphosate and isoxaflutole herbicides. To achieve isoxaflutole tolerance, the cotton expresses Pseudomonas fluorescens 4-hydroxyphenylpyruvate dioxygenase (HPPD), which contains a point mutation that confers reduced binding affinity to HPPD inhibitors. To achieve tolerance to glyphosate, the cotton expresses the Zea mays double mutant 5-enolpyruvylshikimate-3-phosphate synthase, which has a reduced binding affinity to the herbicide.
Le terme « organisme récepteur » désigne un organisme (non modifié ou déjà modifié) ayant fait l'objet d'une modification génétique, tandis que les « organismes parentaux » désignent les organismes impliqués dans un croisement ou une fusion cellulaire pour générer un organisme modifié.
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BCH-ORGA-SCBD-12080-6 Organisme Gossypium hirsutum (Cotton)Cultures
Coker 312
EN
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BCS-GHØØ2-5 - GlyTol™ Cotton GHB614| Bayer CropScience | Résistance aux herbicides (Glyphosate)
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MST-FGØ72-2 - Soy modified for tolerance to glyphosate and HPPD inhibitors| Bayer CropScience LP | HPPD Herbicides, Résistance aux herbicides (Glyphosate)
pTSIH09
EN
- Transgénèse par agrobacterium
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Certains de ces éléments génétiques peuvent être présents sous forme de fragments ou sous forme tronquée. Veuillez consulter les notes ci-dessous, s’il y a lieu.
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BCH-GENE-SCBD-104647-3 Histone H4 gene Promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoteur
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BCH-GENE-SCBD-46333-8 5-enolpyruvylshikimate-3-phosphate synthase | Zea mays (Maize, Corn, MAIZE)Séquence codante de protéines | Résistance aux herbicides (Glyphosate)
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BCH-GENE-SCBD-104648-2 Histone H3 Gene II intron 1 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Intron
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BCH-GENE-SCBD-101419-5 Optimized chloroplast transit peptide | Zea mays (Maize, Corn, MAIZE) | Helianthus annuus (Sunflower, HELAN)Séquence signal
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BCH-GENE-SCBD-104646-4 Histone H4 gene 3' UTR | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Terminateur
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BCH-GENE-SCBD-101900-6 CsVMV promoter | Cassava vein mosaic virus (Cassava vein mosaic virus, CVMV, CsVMV)Promoteur
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BCH-GENE-SCBD-104793-3 4-hydroxyphenylpyruvate dioxygenase | Pseudomonas fluorescens (PSEFL)Séquence codante de protéines | Résistance aux herbicides
Information on the inserted DNA sequences
The transforming plasmid for cotton BCS-GH811-4 contains two gene cassettes: Pseudomonas fluorescens 4-hydroxyphenylpyruvate dioxygenase (hppd) and Zea mays 5-enolpyruvylshikimate-3-phosphate synthase (2mepsps).
(1) The hppd gene is under control of a Cassava vein mosaic virus promoter and Arabidopsis thaliana histone H4 gene terminator (H4A748). A synthetic optimized N-terminal transit peptide was included to target the translated HPPD W336 protein to the chloroplast. High levels of expression are expected due to the nature of the viral promoter.
(2) The 2mepsps gene is under control of an A. thaliana histone H4 promoter and 3'untranslated region. An A. thaliana histone H3 intron was included to enhance the expression of 2mepsps and a synthetic optimized N-terminal transit peptide is also present to direct the protein to the chloroplasts.
Note
The transforming plasmid for cotton BCS-GH811-4 contains two gene cassettes: Pseudomonas fluorescens 4-hydroxyphenylpyruvate dioxygenase (hppd) and Zea mays 5-enolpyruvylshikimate-3-phosphate synthase (2mepsps).
(1) The hppd gene is under control of a Cassava vein mosaic virus promoter and Arabidopsis thaliana histone H4 gene terminator (H4A748). A synthetic optimized N-terminal transit peptide was included to target the translated HPPD W336 protein to the chloroplast. High levels of expression are expected due to the nature of the viral promoter.
(2) The 2mepsps gene is under control of an A. thaliana histone H4 promoter and 3'untranslated region. An A. thaliana histone H3 intron was included to enhance the expression of 2mepsps and a synthetic optimized N-terminal transit peptide is also present to direct the protein to the chloroplasts.
Note
- Molecular characterization of the event indicated that a single copy of the transfer DNA was inserted into the genome without the integration of vector backbone sequences.
- The hppd sequence contains an amino acid substitution (glycine replacement for tryptophan) at position 336. The sequence was then codon-optimized for expression in plants.
- The 2mepsps sequence contains two point mutations: at position102 (substitution of threonine by isoleucine) and position 106 (substitution of proline by serine).
- The optimized transit protein contains the sequence of the RuBisCO small subunit genes of Zea mays and Helianthus annuus with a tyrosine substitution at position 55 and cotton codon optimization.
FR
- Nourriture pour les animaux
- Nourriture
- BCS-GH811-4 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- BCS-GH811-4 - CropLife International Detection Methods Database ( CropLife ) [ English ]
FR
FR
- EUginius: GHB811 [URL] [ English ]
- OECD Biotrack database: GHB811 [URL] [ English ]
- ISAAA GM Approval database: GHB811 [URL] [ English ]
- EFSA (2021). Assessment of cotton GHB811 [PDF] ( European Food Safety Authority, Regulation (EC) No 1829/2003, application EFSA‐GMO‐ES‐2018‐154) ) [ English ]
- FSANZ Safety Assessment. Food derived from Herbicide-tolerant Cotton Line GHB811 (A1097) [PDF] ( Food Standards Australia New Zealand ) [ English ]
- USDA APHIS: Bayer CropScience Petition for a Determination of Nonregulated Status for Herbicide Tolerant Cotton Transformation Event GHB811 [PDF] ( 17_13801p, Animal and Plant Health Inspection Service, United States Department of Agriculture ) [ English ]
- CFIA Novel Food Information - Herbicide Tolerant Cotton Event GHB811 [URL] [ English ]
- FAO GM Foods Platform: BCS-GH811-4 [URL] [ English ]
- CFIA Decision (2018-123). Determination of the safety of BASF Canada Inc. (Gossypium hirsutum) event GHB811 [URL] [ English ]
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