Additional information concerning the cry1Ab gene inserts in this LMO:
The expression of the two copies of the cry1Ab genes are under the control either of a pollen-specific promoter the from a calcium-dependent protein kinase or green tissue-specific promoter phosphoenolpyruvate carboxylase. Both promoters were isolated from maize. The termination sequences for both of genes was from cauliflower mosaic virus (CaMV), a known plant pest.

Additional information concerning the bar gene insert in this LMO:
This LMO contains one copy of the bar gene from Streptomyces hygroscopicus which encodes for phosphinotricin acetyltransferase (PAT) that confers resistance to glufosinate herbicide. The bar gene is under the regulation of the 35S promoter and the 35S terminator from the cauliflower mosaic virus (CaMV).

Additional information concerning the bla gene insert in this LMO:
The bla gene from Escherichia coli is not expressed in plant cells, but was employed as a selectable trait for screening bacterial colonies for the presence of the plasmid vector.

Additional information on the inserted genetic material:

Two plasmids, pCIB3064 and pCIB4431 were used as vectors for the transformation of Bt176 maize. Both are derivatives of the plasmid PUC18, which has a molecular weight of 2.7 kb and contains sequences such as prokaryotic gene bla and gene lacZ. 

Plasmid pCIB3064 contains one copy of the bar gene which is under the regulation of the 35S promoter and the 35S terminator from the cauliflower mosaic virus (CaMV).  The plasmid pCIB4431 contains two copies of a synthetic truncated cry1Ab gene; the first copy which is under the regulation of a promoter derived from maize phosphoenolpyruvate carboxylase gene and the CaMV 35S terminator; and the second copy which is under the regulation of a promoter derived from maize calcium-dependent protein kinase gene (“pollen promoter’) and the CaMV 35S terminator.

There are uncertainties regarding the copy number of the inserts in Bt176. Evidence suggests that 2-5 copies of the inserts may be present (see document below on the molecular characterization of Bt176).