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Organisme vivant modifié
(LMO)
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Bollgard™ cotton
EN
757
Oui
MON-ØØ757-7
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Organisation :Monsanto ()800 North Lindbergh Blvd.St. Louis, MO
63167, États-Unis d'AmériqueTéléphone : + 1 314 694-1000,Télécopieur : +1 314 694-3080,E-mail :Site Web : http://www.monsanto.com,
Cotton line MON757 was genetically engineered to resist cotton bollworm, tobacco budworm and pink bollworm by producing its own insecticide. This line was developed by introducing the cry1Ac gene, isolated from the common soil bacterium Bacillus thuringiensis (Bt), into a cotton line by Agrobacterium-mediated transformation.
EN
Le terme « organisme récepteur » désigne un organisme (non modifié ou déjà modifié) ayant fait l'objet d'une modification génétique, tandis que les « organismes parentaux » désignent les organismes impliqués dans un croisement ou une fusion cellulaire pour générer un organisme modifié.
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BCH-ORGA-SCBD-12080-6 Organisme Gossypium hirsutum (Cotton)Cultures
FR
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MON-89924-2 - Bollgard™ cotton| Monsanto | Résistance aux antibiotiques (Kanamycine), Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites))
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MON-ØØ531-6 - Bollgard™ cotton| Monsanto | Résistance aux antibiotiques (Kanamycine, Néomycine), Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites))
PV-GHBK04
EN
- Transgénèse par agrobacterium
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0.620 kb
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3.500 kb
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0.630 kb
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0.320 kb
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0.790 kb
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0.260 kb
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Certains de ces éléments génétiques peuvent être présents sous forme de fragments ou sous forme tronquée. Veuillez consulter les notes ci-dessous, s’il y a lieu.
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BCH-GENE-SCBD-14986-6 Cry1Ac | Bacillus thuringiensis (Bt, Bacillus, BACTU)Séquence codante de protéines | Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites))
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Séquence codante de protéines | Résistance aux antibiotiques (Kanamycine)
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoteur
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BCH-GENE-SCBD-103856-6 α' subunit of β-conglycinin gene terminator | Glycine max (Soybean, Soya bean, Soya, SOYBN)Terminateur
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminateur
The Cry1Ac coding sequence was modified for plant optimised codons and resulted in a single amino acid change at L766S.
Southern blot analysis indicated that a single complete and intact copy of the expression cassette was integrated into the host genome as well as a 3.8kb partial, non functional fragment located at a separate site from the intact copy consisting of a portion of the Cry1Ac coding sequence and a portion of the 3'UTR.
The plasmid also contains the antibiotic resistance aad gene. This gene confers resistance to the antibiotics spectinomycin and streptomycin, and facilitated the selection of bacteria containing the plasmid in the initial steps of transforming the cotton tissue. The aad gene is under the control of a bacterial promoter. Studies using ELISA testing have indicated that there there is no detectable expression of the aad gene in the modified line.
EN
Southern blot analysis indicated that a single complete and intact copy of the expression cassette was integrated into the host genome as well as a 3.8kb partial, non functional fragment located at a separate site from the intact copy consisting of a portion of the Cry1Ac coding sequence and a portion of the 3'UTR.
The plasmid also contains the antibiotic resistance aad gene. This gene confers resistance to the antibiotics spectinomycin and streptomycin, and facilitated the selection of bacteria containing the plasmid in the initial steps of transforming the cotton tissue. The aad gene is under the control of a bacterial promoter. Studies using ELISA testing have indicated that there there is no detectable expression of the aad gene in the modified line.
FR
- Nourriture
- Nourriture pour les animaux
- Fibre / textile
FR
Cotton line MON757 was genetically engineered to resist cotton bollworm, tobacco budworm and pink bollworm by producing their own insecticide.
The cry1Ac gene produces the insect control protein Cry1Ac, a delta-endotoxin. The Cry1Ac protein produced in MON757 is almost identical to that found in nature and in commercial Bt spray formulations. Cry proteins, of which Cry1Ac is only one, act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow, causing gut paralysis and eventual death due to bacterial sepsis.
Cry1Ac is insecticidal only when eaten by the larvae of lepidopteran insects (moths and butterflies), and its specificity of action is directly attributable to the presence of specific binding sites in the target insects. There are no binding sites for delta-endotoxins of B. thuringiensis on the surface of mammalian intestinal cells, therefore, livestock animals and humans are not susceptible to these proteins.
EN
The cry1Ac gene produces the insect control protein Cry1Ac, a delta-endotoxin. The Cry1Ac protein produced in MON757 is almost identical to that found in nature and in commercial Bt spray formulations. Cry proteins, of which Cry1Ac is only one, act by selectively binding to specific sites localized on the lining of the midgut of susceptible insect species. Following binding, pores are formed that disrupt midgut ion flow, causing gut paralysis and eventual death due to bacterial sepsis.
Cry1Ac is insecticidal only when eaten by the larvae of lepidopteran insects (moths and butterflies), and its specificity of action is directly attributable to the presence of specific binding sites in the target insects. There are no binding sites for delta-endotoxins of B. thuringiensis on the surface of mammalian intestinal cells, therefore, livestock animals and humans are not susceptible to these proteins.
- OECD UID Database [ English ]
- CERA GM Database [ English ]
- Bollgard Cotton - Monsanto.pdf [ English ]
| Type de fichier | Champs | Fichier(s) : | |
|---|---|---|---|
| Décision de pays ou tout autre communication | Identification de l'OVM | 5 | |
| Évaluation des risques générée par un processus de réglementation | Organisme(s) vivant(s) modifié(s) | 5 | |
| Organisme vivant modifié | OVM connexe(s) | 2 | |
| Laboratoire pour la détection et d'identification des OVM | OVM(s) détectable(s) par le laboratoire | 2 | |
