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Organisme vivant modifié
(LMO)
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Roundup Ready™ soybean
EN
GTS 40-3-2 (40-3-2)
Oui
MON-Ø4Ø32-6
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Organisation :Monsanto ()800 North Lindbergh Blvd.St. Louis, MO
63167, États-Unis d'AmériqueTéléphone : + 1 314 694-1000,Télécopieur : +1 314 694-3080,E-mail :Site Web : http://www.monsanto.com,
The soybean line GTS 40-3-2 was developed to allow for the use of glyphosate, the active ingredient in the herbicide Roundup®, as a weed control option. This genetically engineered soybean line contains a form of the plant enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) that allows GTS 40-3-2 to survive the otherwise lethal application of glyphosate. The EPSPS gene put into GTS 40-3-2 was isolated from a strain of the common soil bacterium Agrobacterium tumefaciens called CP4 and the form of EPSPS enzyme produced by this gene is tolerant to glyphosate.
Le terme « organisme récepteur » désigne un organisme (non modifié ou déjà modifié) ayant fait l'objet d'une modification génétique, tandis que les « organismes parentaux » désignent les organismes impliqués dans un croisement ou une fusion cellulaire pour générer un organisme modifié.
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BCH-ORGA-SCBD-10453-8 Organisme Glycine max (Soybean, Soya bean, Soya / soja /соя, SOYBN)Cultures
Line: A5403
EN
PV-GMGT04
EN
- Pistolet biolistique / à particules
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Certains de ces éléments génétiques peuvent être présents sous forme de fragments ou sous forme tronquée. Veuillez consulter les notes ci-dessous, s’il y a lieu.
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BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Séquence codante de protéines | Résistance aux herbicides (Glyphosate)
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoteur
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminateur
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BCH-GENE-SCBD-103899-3 Chloroplast Transit Peptide 4 | Petunia hybrida (Petunia, PETHY)Séquence signal
The plasmid PV-GMGT04 contained three transformation cassettes driven by plant promoters: two Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (cp4-epsps) genes and a gene encoding ß-glucuronidase (GUS) from Escherichia coli. Only a portion of this vector was incorporated into event 40-3-2. Southern blot and PCR analysis indicated that only a single transformation cassette containing the EPSPS coding sequence was integrated into the host genome. The largest insert, containing the function CP4 EPSPS gene, contained a deletion in the enhancer region of the E35S promoter and the remainder of the E35S promoter was functional. Analysis also indicated that there was no integration of segments of the vector backbone, the GUS coding sequence or the CmoVb promoter.
The cp4-epsps coding sequence encodes a 455 amino acid protein is terminated by tandem stop codons, and results in the synthesis of the full length and functional ~46kDa CP4EPSPS protein in Round-up Ready soybean event 40-3-2 as confirmed by western blotting, enzyme-linked immunosorbent assay and EPSPS enzyme activity assays.
The cp4-epsps coding sequence encodes a 455 amino acid protein is terminated by tandem stop codons, and results in the synthesis of the full length and functional ~46kDa CP4EPSPS protein in Round-up Ready soybean event 40-3-2 as confirmed by western blotting, enzyme-linked immunosorbent assay and EPSPS enzyme activity assays.
FR
- Nourriture pour les animaux
- Nourriture
- MON-Ø4Ø32-6 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
- MON-Ø4Ø32-6 - CropLife International Detection Methods Database [ English ]
- EUginius - MON-Ø4Ø32-6 [ English ]
- GMO Detection method Database - MON-Ø4Ø32-6 [ English ]
- MON-Ø4Ø32-6 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- MON-Ø4Ø32-6 - CropLife International Detection Methods Database ( CropLife ) [ English ]
FR
For the Cuban market, the Centro de Ingeniería Genética y Biotecnología has produced hybrid living modified soybeans using the same living modified parental lines. The living modified soybeans are referred to as "SCIGB-L1", "Yagüajay L7", "Carolina RP5", "CEB-2" and "CIGB CC6".
The EPSPS enzyme is part of an important biochemical pathway in plants called the shikimate pathway, that is involved in the production of aromatic amino acids and other aromatic compounds. When conventional plants are treated with glyphosate, the plants cannot produce the aromatic amino acids needed to grow and survive. EPSPS is present in all plants, bacteria, and fungi. It is not present in animals, which do not synthesize their own aromatic amino acids. Because the aromatic amino acid biosynthetic pathway is not present in mammals, birds or aquatic life forms, glyphosate has little if any toxicity for these organisms. The EPSPS enzyme is naturally present in foods derived from plant and microbial sources.
The EPSPS enzyme is part of an important biochemical pathway in plants called the shikimate pathway, that is involved in the production of aromatic amino acids and other aromatic compounds. When conventional plants are treated with glyphosate, the plants cannot produce the aromatic amino acids needed to grow and survive. EPSPS is present in all plants, bacteria, and fungi. It is not present in animals, which do not synthesize their own aromatic amino acids. Because the aromatic amino acid biosynthetic pathway is not present in mammals, birds or aquatic life forms, glyphosate has little if any toxicity for these organisms. The EPSPS enzyme is naturally present in foods derived from plant and microbial sources.
- MON-Ø4Ø32-6 - Monsanto.pdf [ English ]
- MON-Ø4Ø32-6 - OECD [ English ]
| Type de fichier | Champs | Fichier(s) : | |
|---|---|---|---|
| Laboratoire pour la détection et d'identification des OVM | OVM(s) détectable(s) par le laboratoire | 36 | |
| Organisme vivant modifié | Organisme récepteur ou organismes parentaux | 4 | |
| Organisme vivant modifié | OVM connexe(s) | 1 | |
| Décision de pays ou tout autre communication | Identification de l'OVM | 59 | |
| Évaluation des risques générée par un processus de réglementation | Organisme(s) vivant(s) modifié(s) | 50 | |
