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Organisme vivant modifié (LMO)
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BCH-LMO-SCBD-265294-2   |   PDF   |   Imprimer   |  
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Décisions sur l’OVM Évaluations des risques  
published: 06 sept. 2023 last updated: 03 juin 2026
Identité de l’organisme vivant modifié
L’image ci-dessous identifie l’OVM par son identificateur unique, son nom commercial et un lien vers cette page du Centre d’échange Cliquer dessus pour télécharger une plus grande image sur votre ordinateur Pour du soutien sur comment utiliser, aller dans la page de liens rapides OVM.
Insect-resistant and herbicide-tolerant maize
EN
DP910521
Oui
DP-91Ø521-2
The maize (Zea mays) was modified for the resistance to lepidoptera insects and tolerance to herbicides. For resistance to lepidoptera pests, the maize expresses Bacillus thuringiensis Cry1B.34, which forms non-specific, ion conducting pores in the apical membrane of midgut epithelial cells, leading to a disruption of the midgut epithelium and eventual death. For tolerance to glufosinate, the maize expresses Streptomyces viridochromogenes phosphinothricin N-acetyltransferase, which inactivates the herbicide through acetylation. In addition, the maize also contains a gene cassette for Escherichia coli phosphomannose isomerase, which was used as a selectable marker during transformation by allowing for the maize plants to use mannose as a carbon source.
EN
Le terme « organisme récepteur » désigne un organisme (non modifié ou déjà modifié) ayant fait l'objet d'une modification génétique, tandis que les « organismes parentaux » désignent les organismes impliqués dans un croisement ou une fusion cellulaire pour générer un organisme modifié.
Inbred line PH184C
EN
Caractéristiques du processus de modification
PHP71012; PHP79620
EN
  • Pistolet biolistique / à particules
  • Manipulation génétique (ex. : CRISPR-Cas, etc)
 
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Certains de ces éléments génétiques peuvent être présents sous forme de fragments ou sous forme tronquée. Veuillez consulter les notes ci-dessous, s’il y a lieu.
The modified maize contains three gene cassettes: Escherichia coli phosphomannose isomerase (pmi), Streptomyces viridochromogenes phosphinothricin N-acetyltransferase (pat) and Bacillus thuringiensis cry1B.34.

The pmi coding sequence is under control of a Zea mays ubiquitin (ubi) promoter and a Solanum tuberosum proteinase inhibitor II (pinII) terminator. A 5' untranslated region and intron from ubi were included to enhance expression of pmi. An additional terminator (Z. mays 19-kDa zein gene terminator) was added between the gene cassettes to prevent transcriptional interference. Due to the nature of the promoter and presence of enhancers, high levels of transcription are expected.

The pat coding sequence is under control of an Oryza sativa actin 1 promoter and Cauliflower mosaic virus 35S terminator. An intron from the actin 1 gene was also included to enhance transcription levels. An additional two terminators (Sorghum bicolor ubiquitin and gamma kafarin terminators) were added to prevent transcriptional interference between the gene cassettes.

The cry1B.34 coding sequence is under control of a Lamium leaf distortion virus promoter and O. sativa ubiquitin 1 terminator. Two Mirabilis mosaic virus enhancers and Z. mays hydroxyproline-rich glycoprotein 5' untranslated region were added adjacent to the promoter to enhance expression from this gene cassette. Due to the presence of enhancers and the constitutive nature of the promoter, high-levels of transcription are expected.

Note:
  • The size of the Z. mays ubiquitin promoter, 5' untranslated region and intron were not available at the time of record creation.
  • The pat coding sequence was optimized for expression in maize.
  • Cry1B.34 protein is 1,149 amino acids in length and has a molecular weight of approximately 129 kDa.
  • The maize was developed using site-specific integration. The maize was initially transformed with the following plasmids to generate the site-specific "landing pad": PHP71012, PHP70594, PHP21139 and PHP21875. CRISPR-Cas expressed from PHP70594 directs the specific insertion (via homology-directed repair) of the "landing pad" into the maize genome. Following regeneration of the maize plants, a second transformation occurred where microparticle co-bombardment was used to introduce PHP79620, PHP5096, PHP21875 and PHP73572. Transient expression of FLP recombinase replaces some "landing pad" sequences with a recombination fragment. Thus, the final modified maize contains genetic elements from PHP71012 and PHP79620. All plasmids used in the transformations are briefly described below:
    • PHP71012 ("Landing pad"): Z. mays genomic recognition site; Cre recombination site (loxP); Z. mays ubiquitin 1 promoter; flippase recombination site (FRT1); neomycin phosphotransferase II; pinII terminator; flippase recombination site FRT87; genomic recognition site.
    • PHP70594 (transient; not integrated into genome): cas9; guide RNA
    • PHP21139 (transient; not integrated into genome): Z. mays wuschel2 (wus2)
    • PHP21875 (transient; not integrated into genome): Z. mays ovule developmental protein 2 (odp2)
    • PHP79620 (trait genes): FRT1; pmi; pat; loxP; cry1B.34; FRT87
    • PHP5096 (transient; not integrated into genome): flippase recombinase
    • PHP73572 (transient; not integrated into genome): Z. mays wus2
    • PHP21875 (transient; not integrated into genome): Z. mays odp2
  • Southern-by-Sequencing analysis indicated that the modified maize contains a single insert. No unexpected rearrangements, junctions, additions or plasmid backbone sequences were detected.
  • Southern blot analysis indicated that the insertion was stable across five generations.
  • It is recommended to refer to the attached documents in the 'Additional information' section for more information.
EN
Caractéristiques de l’OVM
FR
  • Nourriture pour les animaux
  • Nourriture
Méthode(s) de détection
FR
Informations supplémentaires
During the characterization of DP910521 maize, proprietary maize inbred (PH184C) and F1 hybrid (PH47K2/PH184C) were used as experimental controls.
EN
Les fichiers qui font référence à ce document Montré dans la recherche
Type de fichier Champs Fichier(s) :
Organisme vivant modifié Organisme récepteur ou organismes parentaux 1
Décision de pays ou tout autre communication Identification de l'OVM 6
Évaluation des risques générée par un processus de réglementation Organisme(s) vivant(s) modifié(s) 7

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