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Organisme vivant modifié
(LMO)
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Bollgard II.I™ cotton
EN
MON15947
Oui
MON-15947-5
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Organisation :Monsanto ()800 North Lindbergh Blvd.St. Louis, MO
63167, États-Unis d'AmériqueTéléphone : + 1 314 694-1000,Télécopieur : +1 314 694-3080,E-mail :Site Web : http://www.monsanto.com, -
Organisation :Bayer CropScience ()Téléphone :Télécopieur :E-mail :Site Web : http://www.bayercropscience.com,
The cotton (Gossypium hirsutum) MON15947 is a segregant derived from the cotton MON15985 (Bollgard II™) through conventional breeding, in which the MON531 (Bollgard™) cassette containing the Cry1Ac protein and the NPTII selection marker were segregated out. The MON15947 expresses the Cry2Ab2 crystalline protein from Bacillus thuringiensis subsp. kurstaki, which confers resistance to lepidopteran cotton pests, including the tobacco budworm (Heliothis virescens), pink bollworm (Pectinophora gossypiella), cotton bollworm (Helicoverpa zea), and soybean looper (Pseudoplusia includens).
Le terme « organisme récepteur » désigne un organisme (non modifié ou déjà modifié) ayant fait l'objet d'une modification génétique, tandis que les « organismes parentaux » désignent les organismes impliqués dans un croisement ou une fusion cellulaire pour générer un organisme modifié.
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BCH-ORGA-SCBD-12080-6 Organisme Gossypium hirsutum (Cotton)Cultures
FR
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MON-15985-7 - Bollgard II™ cotton| Monsanto | Résistance aux antibiotiques (Kanamycine, Néomycine), Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites)), Gènes marqueurs et gènes rapporteurs sélectables
PV-GHBK11
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- Croisement
- Autres (Segregation)
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0.613 kb
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1.907 kb
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0.255 kb
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0.614 kb
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1.808 kb
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Certains de ces éléments génétiques peuvent être présents sous forme de fragments ou sous forme tronquée. Veuillez consulter les notes ci-dessous, s’il y a lieu.
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BCH-GENE-SCBD-14988-7 Cry2Ab2 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Séquence codante de protéines | Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites))
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminateur
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BCH-GENE-SCBD-103901-2 HSP 70 5' untranslated leader sequence | Petunia hybrida (Petunia, PETHY)Leader
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoteur
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BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Séquence signal
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BCH-GENE-SCBD-46004-7 Beta-glucuronidase coding sequence | Escherichia coli (ECOLX)Séquence codante de protéines | Gènes marqueurs et gènes rapporteurs sélectables
Information on the inserted DNA sequences
The cry2Ab2 coding sequence is under the control of an enhanced cauliflower mosaic virus (CaMV) 35S promoter and the nopaline synthase (nos) terminator from Agrobacterium tumefaciens. The expression cassette also includes a 5′ leader sequence derived from the HSP70 gene of Petunia hybrida, intended to enhance protein expression, and a chloroplast transit peptide (TS-CTP2) from Arabidopsis thaliana, which directs the translated protein to the chloroplast. The uidA coding sequence is under the control of the same enhanced 35S promoter and nos terminator.
The cry2Ab2 coding sequence is under the control of an enhanced cauliflower mosaic virus (CaMV) 35S promoter and the nopaline synthase (nos) terminator from Agrobacterium tumefaciens. The expression cassette also includes a 5′ leader sequence derived from the HSP70 gene of Petunia hybrida, intended to enhance protein expression, and a chloroplast transit peptide (TS-CTP2) from Arabidopsis thaliana, which directs the translated protein to the chloroplast. The uidA coding sequence is under the control of the same enhanced 35S promoter and nos terminator.
FR
- Nourriture pour les animaux
- Fibre / textile
- Nourriture
Like other delta-endotoxins derived from Bacillus thuringiensis, the Cry2Ab2 protein exerts its insecticidal activity by binding to specific receptors located in the midgut epithelium of susceptible lepidopteran insects. Upon binding, specific cation pores form, disrupting the intestinal ionic balance, causing intestinal paralysis and eventual insect death. The Cry2Ab2 protein is highly selective and is only active against lepidopteran pests of cotton, including the tobacco borer (Heliothis virescens), pink boll (Pectinophora gossypiella), cotton bollworm (Helicoverpa zea), and soybean looper (Pseudoplusia includens).
In addition to the Cry2Ab2 protein that confers insect resistance, the MON-15947-5 event contains the Escherichia coli Beta-glucuronidase (GUS/uidA) visual marker gene, which allows the identification of transformed plants by means of a colorimetric assay that produces a blue color in the transformed cells.
In addition to the Cry2Ab2 protein that confers insect resistance, the MON-15947-5 event contains the Escherichia coli Beta-glucuronidase (GUS/uidA) visual marker gene, which allows the identification of transformed plants by means of a colorimetric assay that produces a blue color in the transformed cells.
The inserted cassette in MON15947 corresponds to the insertion previously obtained by transformation with the KpnI fragment of the PV-GHBK11 vector. The insert contains 5,719 bp derived from this fragment. Relative to the original KpnI fragment, 307 bp at the 5′ end and 66 bp at the 3′ end are absent in the transformed plant. The deduced amino acid sequence of the cry2Ab2 coding region is as expected based on the PV-GHBK11 sequence; however, the inserted β-D-glucuronidase sequence differs by a single amino acid substitution (E377K).
- International Service for the Acquisition of Agri-biotech Applications [ English ]
- EFSA Journal - 2014 - Scientific Opinion for the placing on the market of IR cotton MON 15985 ( MON15985 cotton ) [ English ]
- OECD Biotrack Product Database [ English ]
- EUginius - MON-15947-5 [ English ]
| Type de fichier | Champs | Fichier(s) : | |
|---|---|---|---|
| Décision de pays ou tout autre communication | Identification de l'OVM | 1 | |
| Évaluation des risques générée par un processus de réglementation | Organisme(s) vivant(s) modifié(s) | 2 | |
