DNA insert from DP202216 (vector PHP40099)
The parental maize contains two gene cassettes: Zea mays MADS-box transcription factor zmm28 and Streptomyces viridochromogenes phosphinothricin N-acetyltransferase (pat).
-Transcription of zmm28 is under control of the Z. mays gos2 promoter and the Solanum tuberosum proteinase inhibitor II gene (pinII) terminator. In addition to the zmm28 coding sequence, a Z. mays ubiquitin 1 intron is present at the 5' end of the transcript to enhance gene expression. The gos2 promoter is expected to promote constitutive expression in all plant tissues.
-Transcription of pat is under control of the Z. mays ubiquitin promoter and the pinII terminator. In addition to the pat coding sequence, the Z. mays ubiquitin 1 5' untranslated region and intron were added to the 5' end to enhance gene expression. The ubiquitin promoter is expected to promote high levels of constitutive expression in all plant tissues.

Note:
- The sequence of zmm28 is comprised of the 5’ UTR at bp 2,749-2,808 (60 bp long), coding sequence at bp 2,809-3,564 (756 bp long) and the 3’ UTR at bp 3,565-3,605 (41 bp long).
- The pat coding sequence was optimized for expression in maize.
- Southern-by-Sequencing analysis indicted that the DP202216 line contained a single T-DNA insertion containing the two gene cassettes without any rearrangements or integration of the vector backbone.

DNA insert from DAS40278 (DAS-4Ø278-9) vector pDAS1740
The parental LMO was generated using the Whiskers-mediated transformation method and contains a Sphingobium herbicidovorans aryloxyalkanoate dioxygenase-1 (aad-1) gene cassette.

The aad-1 coding sequence is under the control of a Zea mays ubiquitin gene promoter and Z. mays root preferential cationic peroxidase terminator. Due to the constitutive nature of the ubiquitin promoter, elevated levels of transcription of this gene cassette are expected to occur.

Note: