Biosafety, agronomic performance and insect resistance studies and effects on non-target organisms were carried out in the field (planned release into the environment) in approximately 17 trials in Brazil, numerous trials in the US and around 10 releases in Argentina. This insertion had no effect to alter the potential behavior of the transformed corn to become more invasive than conventional non-GM hybrids, as evidenced by successive field trials in which GM corns were compared with conventional corns. Horizontal gene flow between MIR 162 maize and other species, even those closely related, has practically zero probability. to occur, as wild species related to corn do not occur naturally in Brazil. No significant differences were observed between the populations of non-target organisms when comparing the cultivation of MIR162 Maize and the non-genetically modified maize. The MIR162 corn is efficient to control the evaluated lepidopteran pests, and behaves similarly to the non-genetically modified corn regarding the evaluated agronomic parameters (plant height, ear insertion height, male and female flowering date, disease score, percentage of erect plants, type of grain, color of grain), presenting performance statistically equal to the respective non-genetically modified hybrids under conditions of cultivation of the culture in Brazil.