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Modified Organism
CDC-FLØØ1-2 - CDC Triffid flax modified for herbicide resistance
Record information and status
Record ID
14768
Status
Published
Date of creation
2006-06-05 14:39 UTC (kirsty.mclean.consultant@cbd.int)
Date of last update
2013-01-15 16:50 UTC (dina.abdelhakim@cbd.int)
Date of publication
2013-01-15 16:50 UTC (dina.abdelhakim@cbd.int)

Living Modified Organism identity
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.

LMO name
CDC Triffid flax modified for herbicide resistance
Transformation event
FP967
Unique identifier
CDC-FLØØ1-2
Developer(s)
University of Saskatchewan
Url:University of Saskatchewan
Description
Linseed tolerant to the herbicide sufonylurea through insertion of the acetolactate synthase (als) gene.  Neomycin phosphotransferase II (neo) confers resistance to the antibiotic kanamycin and the nos gene codes for nopaline synthase; these were used as selectable markers.
Recipient Organism or Parental Organisms
The term Recipient organism refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas Parental organisms refers to those that were involved in cross breeding or cell fusion.
Linum usitatissimum - Flax, Flax, Linseed, LINUS
Characteristics of the transformation process
Vector
pGH6 derived from pGV3850
Techniques used for the modification
  • Agrobacterium-mediated DNA transfer
Genetic elements construct
 
Acetohydroxy acid synthase gene promoter
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Acetohydroxy acid synthase gene
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Acetohydroxy acid synthase gene terminator
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Nopaline Synthase Gene Promoter
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Nopaline Synthase Gene
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Nopaline Synthase Gene Terminator
0.00 Kb
 
 
Nopaline Synthase Gene Promoter
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Neomycin Phosphotransferase II
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Nopaline Synthase Gene Terminator
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Further details
LMO characteristics
Modified traits
Common use(s)
  • Food
  • Feed
Additional Information
Additional Information
Sulfonylurea herbicides, such as triasulfuron and metsulfuron-methyl, target and bind to the enzyme acetolactate synthase (ALS) thereby inhibiting the biosynthesis of the branched chain amino acids valine, leucine and isoleucine and resulting in the accumulation of toxic levels of alpha-ketoglutarate.

In addition to its native ALS gene, CDC Triffid contains an als gene from a chlorsulfuron tolerant line of Arabidopsis thaliana. This variant als gene differs from the wild type A. thaliana gene by one nucleotide and the resulting ALS enzyme differs by one amino acid from the wild type ALS enzyme. The inserted als gene is linked to its native promoter and terminator.

Enzyme extracts from CDC Triffid exhibited a slightly higher ALS activity compared to its non-modified counterpart cv. Norlin. Whereas the statistical significance of this higher activity could not be verified, it may be expected due to the presence of at least two additional copies of the als gene in CDC Triffid.
Other relevant website address or attached documents

Records referencing this document (15)
IDDescription
15record(s) found
Country's Decision or any other Communication2 records
Organization12 records
Risk Assessment1 record