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Living Modified Organism
(LMO)
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Potato modified for the expression of synthetic VP60 for vaccine production against RHDV
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Désirée 35SVP60SEK #17 #6
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Organization:University of Rostock ()Academic or research institute, Agrobiotechnology - Faculty of Agricultural and Environmental SciencesFaculty of Agricultural and Environmental Sciences Justus-von-Liebig-Weg 6Rostock,
18059, GermanyPhone: +49 381 498 3001,Fax:Email: dekan.auf@uni-rostock.de,
Vaccines against rabbit hemorrhagic disease virus (RHDV) are derived from transgenic potato plants modified to express RHDV VP60. Rabbits immunized with leaf extracts from modified plants showed high anti-VP60 antibody titers and were fully protected against the hemorrhagic disease.
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The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12106-6 Organism Solanum tuberosum (Potato, SOLTU)Crops
Cultivar/ Variety: Désirée, Albatros
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p35S (pLH9000-derivative)
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- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-102024-3 Viral protein 60 | Rabbit hemorrhagic disease virus (RHDV)Protein coding sequence | Production of medical or pharmaceutical compounds (human or animal) (Vaccines)
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BCH-GENE-SCBD-102033-4 SEKDEL ER retention signalTransit signal
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BCH-GENE-SCBD-15001-5 Neomycin Phosphotransferase II | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Kanamycin)
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
To enable expression of the VP60 gene in potatoes, a synthetic VP60 gene was introduced into potato plants via Agrobacterium tumefaciens-mediated gene transfer. This gene contains the sequence information of the VP60 gene from the RHDV virus isolate R-592, and codon-optimised using the tobacco genetic code. The synthetic gene fragment is 75% identical to the nucleotide sequence of VP60-gene and 100% identical to the amino acid sequence of the VP60 protein.
A DNA fragment that encodes the SEKDEL amino acids was added to the VP60 C-terminus sequence, thereby effecting the retention of the protein to the endoplasmic reticulum in the secretory pathway of protein synthesis. This is aimed at stabilising the protein.
In the plant, the expression is regulated by the 35S promoter from the cauliflower mosaic virus leading to expression in all plant parts throughout the entire lifecycle of the potato.
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A DNA fragment that encodes the SEKDEL amino acids was added to the VP60 C-terminus sequence, thereby effecting the retention of the protein to the endoplasmic reticulum in the secretory pathway of protein synthesis. This is aimed at stabilising the protein.
In the plant, the expression is regulated by the 35S promoter from the cauliflower mosaic virus leading to expression in all plant parts throughout the entire lifecycle of the potato.
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- Research
- Vaccine
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- Protection of Rabbits against Rabbit Hemorrhagic Disease Virus by Immunization with the VP60 Protein Expressed in Plants with a Potyvirus-Based Vector [ English ]
- Immunisation with potato plants expressing VP60 protein protects against rabbit haemorrhagic disease virus..pdf [ English ]
- Expression of the Viral Antigen VP60 in Transgenic Potatoes and its Effect on the Nutritional Composition of Tubers.pdf [ English ]
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