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Living Modified Organism
(LMO)
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Black Nightshade with reduced pathogenesis-related protein 1S synthesis
EN
S03/144-2
No
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Organization:Max Planck Institute for Chemical Ecology (MPICE)Academic or research instituteHans-Knöll-Strasse 8Jena,
07745, GermanyPhone: +49 3641 57-0,Fax: +49 3641 57-1002,Email: info@ice.mpg.de,Website: https://www.ice.mpg.de,
In the transgenic plants of the line S03/144-2 the synthesis of the plant's pathogenesis-related (PR) protein 1S is to be reduced by post transcriptional gene silencing.
The pr-1S gene derived from Solanum nigrum codes for a PR protein. PR proteins are encoded by the host plant in response to pathogen attacks or comparable stress factors.
For this purpose, a construct was developed in which two complementary internal fragments of the pr-1S gene derived from Solanum nigrum were arranged in sense and antisense orientation, separated by a spacer. As a result of the genetic modification, the resistance of the transgenic plant to phytopathogenic microorganisms is reduced by targeted reduction of the formation of the PR-1S protein.
EN
The pr-1S gene derived from Solanum nigrum codes for a PR protein. PR proteins are encoded by the host plant in response to pathogen attacks or comparable stress factors.
For this purpose, a construct was developed in which two complementary internal fragments of the pr-1S gene derived from Solanum nigrum were arranged in sense and antisense orientation, separated by a spacer. As a result of the genetic modification, the resistance of the transgenic plant to phytopathogenic microorganisms is reduced by targeted reduction of the formation of the PR-1S protein.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-47832-5 Organism Solanum nigrum (Black nightshade, SOLNI)Crops
EN
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Black Nightshade with reduced pathogenesis-related protein 1S synthesis.| Resistance to antibiotics (Hygromycin), Resistance to diseases and pests
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Black nightshade modified for reduced production of endogenous prosystemin| Resistance to antibiotics (Hygromycin), Resistance to diseases and pests
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Black nightshade modified for reduced production of endogenous proteinase inhibitors| Resistance to antibiotics (Hygromycin), Resistance to diseases and pests (Insects)
pSOL3PRIS
EN
- Agrobacterium-mediated DNA transfer
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0.000 kb
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0.295 kb
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0.785 kb
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0.295 kb
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0.000 kb
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0.000 kb
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0.000 kb
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0.000 kb
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-14991-8 Hygromycin B phosphotransferase gene | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Hygromycin),Selectable marker genes and reporter genes
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-103123-6 Pyruvate orthophosphate dikinase, Intron 3 | Flaveria trinervia (Clustered Yellowtops, speedyweed, flaveria, yellow twinstem)Intron
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BCH-GENE-SCBD-108712-1 Pathogenesis-related protein 1S gene | Solanum nigrum (Black nightshade, SOLNI)Protein coding sequence | Resistance to diseases and pests
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
The plasmid pSOL3PRIS used for transformation includes a construct, in which two complementary 295 bp long internal fragments of the pr-1S gene derived from Solanum nigrum were arranged in sense and antisense orientation, separated by a spacer. The 785 bp long third intron of the pyruvate orthophosphate dikinase gene (pdk i3) of Flaveria trinervia, of which splicing activity is suspected, was used as a spacer. Expression is controlled by the promoter and the terminator of the 35S gene of the cauliflower mosaic virus (CaMV).
The hygromycin phosphotransferase gene (hptII) of Escherichia coli expressed under the control of the promoter and terminator of the nopaline synthase gene of Agrobacterium tumefaciens was used as a selection marker. The introduced nucleic acid is integrated in the genome of the recipient organism.
EN
The hygromycin phosphotransferase gene (hptII) of Escherichia coli expressed under the control of the promoter and terminator of the nopaline synthase gene of Agrobacterium tumefaciens was used as a selection marker. The introduced nucleic acid is integrated in the genome of the recipient organism.
EN
- Research
EN
EN
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