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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Black nightshade modified for reduced production of endogenous prosystemin
EN
S03/71; S03/82
No
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Organization:Max Planck Institute for Chemical Ecology (MPICE)Academic or research instituteHans-Knöll-Strasse 8Jena,
07745, GermanyPhone: +49 3641 57-0,Fax: +49 3641 57-1002,Email: info@ice.mpg.de,Website: https://www.ice.mpg.de,
Black nightshade was modified to reduce the synthesis of the plant’s native prosystemin by post-transcriptional gene silencing using an RNAi construct which was developed from fragments of the prosystemin gene from S. nigrum
The prosystemin gene codes for the 200 amino acid prosystemin peptide, which is the precursor of the active 18 amino acid systemin peptide. Systemin is a signaling molecule which plays an important role in mediating the systemic wound response in plant tissues.
The modification resulted in the targeted reduction of the production of the prosystemin peptide leading to a decrease in the plant's defences against herbivores/pathogen infestation.
EN
The prosystemin gene codes for the 200 amino acid prosystemin peptide, which is the precursor of the active 18 amino acid systemin peptide. Systemin is a signaling molecule which plays an important role in mediating the systemic wound response in plant tissues.
The modification resulted in the targeted reduction of the production of the prosystemin peptide leading to a decrease in the plant's defences against herbivores/pathogen infestation.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-47832-5 Organism Solanum nigrum (Black nightshade, SOLNI)Crops
Cultivar/breeding line: Sn 30
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Black nightshade modified for reduced production of endogenous proteinase inhibitors| Resistance to antibiotics (Hygromycin), Resistance to diseases and pests (Insects)
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Black Nightshade with reduced pathogenesis-related protein 1S synthesis| Resistance to antibiotics (Hygromycin), Resistance to diseases and pests
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Black Nightshade with reduced pathogenesis-related protein 1S synthesis.| Resistance to antibiotics (Hygromycin), Resistance to diseases and pests
pSOL3SYS
EN
- Agrobacterium-mediated DNA transfer
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-110670-2 Prosystemin gene | Solanum nigrum (Black nightshade, SOLNI)Protein coding sequence | Resistance to diseases and pests
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BCH-GENE-SCBD-103123-6 Pyruvate orthophosphate dikinase, Intron 3 | Flaveria trinervia (Clustered Yellowtops, speedyweed, flaveria, yellow twinstem)Intron
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-100270-6 Nopaline Synthase Gene Promoter | Agrobacterium tumefaciens (Agrobacterium)Promoter
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BCH-GENE-SCBD-14991-8 Hygromycin B phosphotransferase gene | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Hygromycin),Selectable marker genes and reporter genes
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
The plasmid pSOL3SYS used for transformation includes a construct in which two complementary 404 bp fragments of the prosystemin gene (nigpro) derived from S. nigrum were arranged in sense and antisense orientation, separated by a spacer. The 785 bp long third intron of the pyruvate orthophosphate dikinase gene (pdk i3) of Flaveria trinervia (Asteraceae), of which splicing activity is suspected, was used as a spacer. The expression is controlled by the promoter and the termination signal of the 35S gene of cauliflower mosaic virus (CaMV).
The hygromycin phosphotransferase gene (hptII) of Escherichia coli expressed under the control of the promoter and termination signal of the nopaline synthase gene of Agrobacterium tumefaciens was used as a selection marker. The introduced nucleic acid is integrated in the genome of the recipient organism.
EN
The hygromycin phosphotransferase gene (hptII) of Escherichia coli expressed under the control of the promoter and termination signal of the nopaline synthase gene of Agrobacterium tumefaciens was used as a selection marker. The introduced nucleic acid is integrated in the genome of the recipient organism.
EN
- Research
EN
EN
- Solanum nigrum: a model ecological expression system and its tools [ English ]
- B/DE/07/0188 - SNIF [ English ]
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