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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Herbicide-tolerant, insect-resistant cotton
EN
MON88702 × MON15985 × COT102 × MON88701 × MON88913
Yes
MON-887Ø2-4 × MON-15985-7 × SYN-IR1Ø2-7 × MON-887Ø1-3 × MON-88913-8
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Person:Bayer CropScience Bayer CropSciencePhone:Fax:Email:Website:Related OrganizationBayer CropScience ()Phone:Fax:Email:Website:
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Organization:Syngenta ()Phone:Fax:Email:
The modified cotton (Gossypium hirsutum) was produced through crossing modified parental lines to achieve insect-resistance and herbicide-tolerance. For resistance to Lepidoptera insect pests, the cotton expresses Bacillus thuringiensis subsp. kurstaki crystal protein (delta-endotoxin) Cry1Ac and Cry2Ab2, as well as vegetative insecticidal protein 3A. For protection against Hemipteran and Thysanopteran insect pests, the cotton expresses modified crystal protein mCry51Aa2 from Bacillus thuringiensis strain EG2934. For tolerance to dicamba, the cotton expresses Stenotrophomonas maltophilia dicamba monooxygenase, which inactivates the herbicide via oxidative demethylation. For tolerance to glufosinate, the cotton expresses Streptomyces hygroscopicus phosphinothricin N-acetyltransferase, which inactivate the herbicide via acetylation. For tolerance to glyphosate, the cotton expresses Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase, which has a low binding affinity for the herbicide and thus allows for the continued synthesis of aromatic amino acids and compounds (shikimate pathway). In addition, the modified cotton also contains three selectable markers from Escherichia coli: neomycin phosphotransferase II (kanamycin resistance), hygromycin B phosphotransferase (hygromycin resistance) and beta-glucuronidase (colorimetric marker).
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-ORGA-SCBD-12080-6 Organism Gossypium hirsutum (Cotton)Crops
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BCH-LMO-SCBD-114159-4 Living Modified Organism MON-887Ø2-4 - Insect-protected cotton This document has been updated. This is not the latest published version. Click here to view the latest version of the record.Monsanto | Resistance to diseases and pests (Insects)
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BCH-LMO-SCBD-14774-19 Living Modified Organism MON-15985-7 - Bollgard II™ cottonMonsanto | Resistance to antibiotics (Kanamycin, Neomycin), Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths)), Selectable marker genes and reporter genes
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BCH-LMO-SCBD-14992-9 Living Modified Organism SYN-IR1Ø2-7 - VIPCOT™ CottonSyngenta | Resistance to antibiotics (Hygromycin), Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths)), Selectable marker genes and reporter genes
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BCH-LMO-SCBD-105602-3 Living Modified Organism MON-887Ø1-3 - Dicamba- and Glufosinate-tolerant cottonMonsanto | Resistance to herbicides (Glufosinate)
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BCH-LMO-SCBD-15168-16 Living Modified Organism MON-88913-8 - Roundup Ready™ Flex™ cottonMonsanto | Resistance to herbicides (Glyphosate)
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PV-GHIR508523; PV-GHBK11; PV-GHBK04; pCOT-1; PV-GHHT6997; PV-GHGT35
EN
- Cross breeding
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-101507-5 FMV 34S promoter | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)Promoter
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BCH-GENE-SCBD-114158-2 Heat shock protein 81-2 promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoter
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BCH-GENE-SCBD-114157-2 mCry51Aa2 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects)
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BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminator
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BCH-GENE-SCBD-103856-6 α' subunit of β-conglycinin gene terminator | Glycine max (Soybean, Soya bean, Soya, SOYBN)Terminator
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BCH-GENE-SCBD-14986-6 Cry1Ac | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-101416-6 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-100366-6 CaMV Enhanced 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-15033-8 3"(9)-O-aminoglycoside adenyltransferase | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Streptomycin)
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-111595-1 Neomycin phosphotransferase II promoter | Escherichia coli (ECOLX)Promoter
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-103901-2 HSP 70 5' untranslated leader sequence | Petunia hybrida (Petunia, PETHY)Leader
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BCH-GENE-SCBD-100365-6 Chloroplast transit peptide 2 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Transit signal
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BCH-GENE-SCBD-14988-7 Cry2Ab2 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-46004-7 Beta-glucuronidase coding sequence | Escherichia coli (ECOLX)Protein coding sequence | Selectable marker genes and reporter genes
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BCH-GENE-SCBD-14991-8 Hygromycin B phosphotransferase gene | Escherichia coli (ECOLX)Protein coding sequence | Resistance to antibiotics (Hygromycin),Selectable marker genes and reporter genes
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BCH-GENE-SCBD-101874-2 Ubiquitin gene 3 promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoter
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BCH-GENE-SCBD-104517-2 Actin 2 promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoter
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BCH-GENE-SCBD-14990-5 Vegetative insecticidal protein 3A | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-104662-3 PCSV Promoter | Peanut chlorotic streak virus (PCSV, PClSV)Promoter
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BCH-GENE-SCBD-104664-2 TEV 5' Untranslated Region | Tobacco etch virus (TEV)Leader
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BCH-GENE-SCBD-100728-3 Dicamba monooxygenase gene | Stenotrophomonas maltophilia (S. maltophilia, Stenotrophomonas)Protein coding sequence | Resistance to herbicides
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BCH-GENE-SCBD-105600-1 E6 gene terminator | Gossypium barbadense (Sea-island cotton, Egyptian cotton, GOSBA)Terminator
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BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)Protein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-105196-2 FMV 35S Enhancer | Figwort mosaic virus (Figwort mottle virus, FMV, CMoVb)Leader
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BCH-GENE-SCBD-103903-1 Elongation factor EF-1alpha promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoter
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BCH-GENE-SCBD-103904-1 Elongation factor EF-1alpha Leader | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Leader
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BCH-GENE-SCBD-103905-1 Elongation factor EF-1alpha Intron 1 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Intron
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BCH-GENE-SCBD-14979-7 5-enolpyruvylshikimate-3-phosphate synthase gene | Agrobacterium tumefaciens (Agrobacterium)Protein coding sequence | Resistance to herbicides (Glyphosate)
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BCH-GENE-SCBD-101877-5 rbcS-E9 gene terminator | Pisum sativum (Garden pea, PEA)Terminator
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BCH-GENE-SCBD-105197-2 CaMV 35S Enhancer | Cauliflower mosaic virus (CaMV)Leader
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BCH-GENE-SCBD-103907-3 Actin 8 promoter | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Promoter
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BCH-GENE-SCBD-103908-4 Actin 8 Leader sequence | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Leader sequence
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BCH-GENE-SCBD-103909-3 Actin 8 Intron 1 | Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Intron
DNA insert from MON88702 vector PV-GHIR508523:
The parental cotton genome contains one gene cassette: Bacullis thuringiensis mCry51Aa2.
Transcription of mCry51Aa2 coding sequence directed by the Arabidopsis thaliana heat shock protein 81-2 promoter (P-hsp81-2), with enhanced activity due to the Figwort mosaic virus 34S enhancer (E-FMV) located adjacent to the promoter. Transcription is terminated by a Cauliflower mosaic virus (CaMV) 35S terminator (T-CaMV). Due to the enhancer, high levels of expression are expected.
Note:
The parental cotton genome contains one gene cassette: Bacullis thuringiensis mCry51Aa2.
Transcription of mCry51Aa2 coding sequence directed by the Arabidopsis thaliana heat shock protein 81-2 promoter (P-hsp81-2), with enhanced activity due to the Figwort mosaic virus 34S enhancer (E-FMV) located adjacent to the promoter. Transcription is terminated by a Cauliflower mosaic virus (CaMV) 35S terminator (T-CaMV). Due to the enhancer, high levels of expression are expected.
Note:
- Next-generation sequencing confirmed the vector backbone was not incorporated into the plant and the presence of a single T-DNA integration site.
- The plasmid PV-GHIR508523 contained two T-DNA regions, one of which was crossed out due to selective breeding and was not present in the final cotton line. See the parental LMO record for more information.
- MCry51Aa2 is a 302 amino acid, 33.6kDa single polypeptide. This modified protein contains eight amino acid substitutions (F46S, Y54H, S95A, F147S, Q149E, S167R, P219R, R273W) and a deletion of three amino acids (Δ196-198). Immunoblotting of leaf samples and enzyme-linked immunosorbent assay (ELISA) of leaf, pollen, root, and seed samples confirmed the expression of the protein.
DNA insert from MON15985 vectors PV-GHBK11and PV-GHBK04:
Information on the inserted DNA sequences from vector PV-GHBK11
Transformation with vector PV-GHBK11 inserted two gene cassettes into the modified parental line MON531: Bacillus thuringiensis cry2Ab2 and Escherichia coli beta-glucuronidase (uidA).
The cry2Ab2 sequence is under control of a Cauliflower mosaic virus (CaMV) 35S enhanced promoter and an Agrobacterium tumefaciens nopaline synthase (nos) terminator. A Petunia hybrida heat shock protein 70 leader for enhanced expression and an Arabidopsis thaliana chloroplast transit peptide 2 for targeting translated protein to the chloroplast are also present. The uidA coding sequence is under control of the CaMV 35S enhanced promoter and nos terminator.
Note:
- Due to the constitutive nature of the viral promoters, high levels of expression are expected in all plant tissues.
- Southern blot analysis indicated that a single T-DNA construct had integrated into the genome. The CaMV 35S promoter that drives the expression of the uidA gene was truncated, however this did not affect the expression of the gene. The analysis also showed that there was no integration of any portions of the vector backbone.
Pre-existing DNA insert from MON531 vector PV-GHBK04
The parental organism (MON531) contained two gene cassettes: B. thuringiensis cry1Ac and E. coli neomycin phosphotransferase II (nptII).
The cry1Ac coding sequence is under control of CaMV 35S enhanced promoter and Glycine max α' subunit of β-conglycinin terminator. See notes below about the partial cry1Ac cassette and antibiotic resistance gene aad. The nptII coding sequence is under control of a CaMV 35S promoter and and Agrobacterium tumefaciens nopaline synthase terminator.
Note:
- Due to the constitutive nature of the viral promoters, high levels of expression are expected in all plant tissues.
- The cry1Ac coding sequence was codon-optimized for expression in plants. The codon optimization resulted in a single amino acid change (leucine substituted for serine) at position 766 (L766S).
- Southern blot analysis indicated that the LMO contains a single active copy and a partial inactive copy of the cry1Ac gene both of which are linked.
- The T-DNA contains the antibiotic resistance 3"(9)-O-aminoglycoside adenylyltransferase (aad) gene, which facilitated the selection of bacteria containing the plasmid in the initial steps of transforming the cotton tissue. The gene is under control of a bacterial promoter, which is not active in plant tissues.
DNA insert from COT102 vector pCOT-1:
The DNA insert from COT102 contains two gene cassettes: Escherichia coli hygromycin B phosphotransferase (hpt) and Bacillus thuringiensis vegetative insecticidal protein 3A (vip3Aa).
Transcription of hpt is under control of the Arabidopsis thaliana ubiquitin 3 promoter and the Agrobacterium tumefaciens nopaline synthase (nos) terminator. The gene cassette is present in the counterclockwise orientation. The expression of Bacillus thuringiensis vip3Aa is under transcriptional control of the A. thaliana actin 2 promoter and the nos terminator.
Note:
- Transcription is expected to occur at elevated levels due to the constitutive nature of the promoters.
- The coding sequence of the vip3Aa was optimized for expression in plant cells.
- Southern blot analysis indicated the incorporation of a single copy of the transgene cassettes without the integration of the vector backbone sequences.
- Western blot analysis confirmed the expression of the proteins.
DNA insert from MON88701 vector PV-GHHT6997:
The modified cotton contains two gene cassettes: Stenotrophomonas maltophilia dicamba monooxygenase (dmo) and Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (bar).
The dmo coding sequence is under control of a Peanut chlorotic streak virus promoter and Gossypium barbadense E6 terminator. A Tobacco etch virus 5' untranslated region was added between the promoter and dmo coding sequence to enhance transcription of dmo. The bar coding sequence is under control of a Cauliflower mosaic virus 35S enhanced promoter and an Agrobacterium tumefaciens nopaline synthases terminator. A Petunia hybrida heat shock protein 70 leader was added between the viral promoter and the bar coding sequence to enhance expression of bar.
Note:
- Southern Blot and sequence analysis indicated that the transformation cassette was inserted into the MON88701 genome at a single intact locus containing all the genetic elements without the integration of vector backbone sequences.
DNA insert from MON88913 vector PV-GHGT35:
The transforming plasmid PV-GHGT35 carried a transfer DNA sequence comprising of two codon-optimised Agrobacterium tumefaciens 5-enolpyruvylshikimate-3-phosphate synthase (epsps) cassettes:
- the first epsps coding sequence under the regulation of a chimeric transcriptional promoter (Figwort mosaic virus 35S promoter enhancer and Arabidopsis thaliana elongation factor EF-1 alpha (tsf1) promoter), tsf1 leader and intron sequences, an A. thaliana chloroplast transit peptide 2 sequence and a Pisum sativum ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco) E9 transcript termination and polyadenylation sequence (T-E9).
- the second epsps coding sequence regulated by another chimeric transcriptional promoter (Cauliflower mosaic virus 35S enhancer and A. thaliana actin 8 (act8) promoter), act8 leader and intron sequences, A. thaliana chloroplast targeting peptide 2 and T-E9.
High levels of transcription are expected from both cassettes due to the presence of viral enhancer sequences. The EPSPS protein is expected to accumulate in the chloroplast due to the transit signal peptide.
Note:
Note:
- The promoter for both genetic constructs are chimeric promoters containing viral enhancer sequences. Thus, the size of the promoters (Elongation factor 1 alpha and Actin 8) in the 'Genetic elements construct' reflects the size of chimeric promoters (FMV 35S enhancer + Elongation factor 1 alpha promoter; CaMV 35S enhancer + Actin 8 promoter).
For more information, kindly refer to the parental LMO records.
EN
- Feed
- Fiber/textile
- MON-15985-7 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- MON-887Ø1-3 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- MON-88913-8 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- SYN-IR1Ø2-7 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- MON-15985-7 - CropLife International Detection Methods Database ( CropLife ) [ English ]
- SYN-IR1Ø2-7 - CropLife International Detection Methods Database ( CropLife ) [ English ]
- MON-887Ø1-3 - CropLife International Detection Methods Database ( CropLife ) [ English ]
- MON-88913-8 - CropLife International Detection Methods Database ( CropLife ) [ English ]
EN
The crystal proteins Cry1Ac and Cry2Ab2 target Lepidopteran pests, including tobacco budworm (Heliothis virescens), pink bollworm (Pectinophora gossypiella), cotton bollworm (Helicoverpa zea), cabbage looper (Trichoplusia ni), saltmarsh caterpillar (Estigmene acrea), cotton leaf perforator (Bucculatrix thurbeiella), soybean looper (Pseudoplusia includens), beet armyworm (Spodoptera exigua), fall armyworm (Spodoptera frugiperda), yellowstriped armyworm (Spodoptera ornithogolli) and European corn borer (Ostrinia nubilalis).
The modified crystal protein mCry51Aa2 from Bacillus thuringiensis strain EG2934 to confer protection against Hemipteran and Thysanopteran insect pests, particularly tarnished plant bugs (Lygus hesperus and Lygus lineolaris), cotton fleahopper (Pseudoalomoscelis seriatus) and thrips ( Frankiniella spp.).
Crystal proteins have a pore-forming mode of action in the epithelial lining of the midgut of feeding insects. The cation-specific pores formed disrupt the midgut ionic equilibrium, leading to gut paralysis and eventual death. Bacterial sepsis additionally contributes to the death of insect.
The modified crystal protein mCry51Aa2 from Bacillus thuringiensis strain EG2934 to confer protection against Hemipteran and Thysanopteran insect pests, particularly tarnished plant bugs (Lygus hesperus and Lygus lineolaris), cotton fleahopper (Pseudoalomoscelis seriatus) and thrips ( Frankiniella spp.).
Crystal proteins have a pore-forming mode of action in the epithelial lining of the midgut of feeding insects. The cation-specific pores formed disrupt the midgut ionic equilibrium, leading to gut paralysis and eventual death. Bacterial sepsis additionally contributes to the death of insect.
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