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Organisme vivant modifié
(LMO)
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Herbicide-tolerant, insect-resistant cotton
EN
T304-40
Oui
BCS-GHØØ4-7
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Organisation :Bayer Crop Science K.K ()Marunouchi Kitaguchi Building, 1-6-5, MarunouchiChiyoda-ku, Tokyo
, JaponTéléphone :Télécopieur :E-mail :
The cotton (Gossypium hirsutum) was modified for resistance against lepidopteran insect pests and glufosinate-ammonium tolerance. To achieve resistance against Lepidoptera, the cotton expresses the cry1Ab gene from Bacillus thuringiensis subsp. kurstaki, which produces a crystal delta-endotoxin that binds to specific midgut receptors in the insect larva, forming pores that disrupt osmotic balance and cause fatal cell lysis. To achieve glufosinate tolerance, the cotton expresses Streptomyces hygroscopicus phosphinothricin N-acetyltransferase encoding pat gene, which inactivates the active compound L-phosphinothricin through acetylation of the primary amino group.
Le terme « organisme récepteur » désigne un organisme (non modifié ou déjà modifié) ayant fait l'objet d'une modification génétique, tandis que les « organismes parentaux » désignent les organismes impliqués dans un croisement ou une fusion cellulaire pour générer un organisme modifié.
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BCH-ORGA-SCBD-12080-6 Organisme Gossypium hirsutum (Cotton)Cultures
Cultivar Coker 315
EN
pTDL008 derived from pGSV20
EN
- Transgénèse par agrobacterium
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Certains de ces éléments génétiques peuvent être présents sous forme de fragments ou sous forme tronquée. Veuillez consulter les notes ci-dessous, s’il y a lieu.
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BCH-GENE-SCBD-14972-12 Phosphinothricin N-acetyltransferase gene | Streptomyces hygroscopicus (STRHY)Séquence codante de protéines | Résistance aux herbicides (Glufosinate)
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BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)Séquence codante de protéines | Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites))
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BCH-GENE-SCBD-101021-3 Ps7s7 | Subterranean clover stunt virus (SCSV, Subterranean clover stunt virus)Promoteur
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BCH-GENE-SCBD-101025-5 NADP-malic enzyme 1 gene 3'UTR and terminator | Flaveria bidentis (Coastal plain yellowtops, FLABI)Terminateur
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoteur
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminateur
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BCH-GENE-SCBD-101416-7 Ti plasmid right border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-101415-9 Ti plasmid left border repeat | Agrobacterium tumefaciens (Agrobacterium)Plasmid vector
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BCH-GENE-SCBD-104947-3 5'e1 Leader | Oryza sativa (Rice, ORYSA)Leader
Information on the inserted DNA sequences
The transforming plasmid pTDL008 contains two gene cassettes: Bacillus thuringiensis crystal protein cry1Ab and Streptomyces hygroscopicus phosphinothricin N-acetyltransferase (bar).
(1) The cry1Ab coding sequence is under the control of Subterranean clover stunt virus genome segment 7 (Ps7s7) promoter and Flaveria bidentis NADP-malic enzyme 1 terminator. The 5' leader sequence from Oryza sativa E1 gene was also included in the cassette to enhance the expression of cry1Ab. High levels of transcription is expected due to the viral promoter.
(2) The bar coding sequence is under the control of a Cauliflower mosaic virus 35S promoter and Agrobacterium tumefaciens nopaline synthase terminator. High levels of transcription is expected due to the viral promoter.
Note
(1) The cry1Ab coding sequence is under the control of Subterranean clover stunt virus genome segment 7 (Ps7s7) promoter and Flaveria bidentis NADP-malic enzyme 1 terminator. The 5' leader sequence from Oryza sativa E1 gene was also included in the cassette to enhance the expression of cry1Ab. High levels of transcription is expected due to the viral promoter.
(2) The bar coding sequence is under the control of a Cauliflower mosaic virus 35S promoter and Agrobacterium tumefaciens nopaline synthase terminator. High levels of transcription is expected due to the viral promoter.
Note
- The cry1Ab gene has been derived from Bacillus thuringiensis strain berliner 1715 (Genbank accession No. X04698 - first cloned and characterised by Höfte et al (1986)).
- Sequencing of the 9056 bp inserted transgenic construct and Southern blot analysis revealed a truncated T-DNA construct (with an incomplete me1 terminator) was inserted into the T304-40 LM cotton line in addition to: (1) a partial 3’me1 terminator; (2) a partial copy of the cry1Ab gene cassette, with a truncated Ps7s7 promoter, in a tail-to-tail orientation; (3) a partial copy of the bar gene cassette in which the nos terminator is truncated.
- As a result of the transformation event, four new junctions were created (see Figure 4 in the attached file below), two being located at the 5’ and 3’ ends of the insert and two being located within the insert as a result of the rearrangement.
- Southern blot analysis of the genomic DNA from COT102 revealed the incorporation of single intact copies of the vip3Aa and hpt genes, along with intact copies of their respective regulatory sequences. Results of these analyses also demonstrated that none of the vector backbone sequences, including the streptomycin adenyltransferase (aadA) gene, were incorporated into the genomic DNA.
FR
- Nourriture pour les animaux
- Fibre / textile
- Nourriture
- Autres (Industrial)
- BCS-GHØØ4-7 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
FR
FR
- EUginius: T304-40 [URL] [ English ]
- OECD Biotrack database: T304-40 [URL] [ English ]
- FSANZ Safety assessment. Insect protected and herbicide tolerant cotton line T304-40 (A1028) [PDF] ( Food Standards Australia and New Zealand ) [ English ]
- COGEM advice. Import and processing of cotton T304-40 (CGM/120105-01) [PDF] [ English ]
- ISAAA GM Approval database: T304-40 [URL] [ English ]
- FAO GM Foods Platform: BCS-GHØØ4-7 [URL] [ English ]
- CFIA Novel Food Information: Cotton Event T304-40 [URL] [ English ]
| Type de fichier | Champs | Fichier(s) : | |
|---|---|---|---|
| Organisme vivant modifié | Organisme récepteur ou organismes parentaux | 8 | |
| Organisme vivant modifié | OVM connexe(s) | 1 | |
| Décision de pays ou tout autre communication | Identification de l'OVM | 15 | |
| Évaluation des risques générée par un processus de réglementation | Organisme(s) vivant(s) modifié(s) | 14 | |
| Laboratoire pour la détection et d'identification des OVM | OVM(s) détectable(s) par le laboratoire | 3 | |
