Loading...
Cet enregistrement a été supprimé. Comme ce document est lié à un enregistrement publié, il est disponible ici à titre de référence uniquement.
Cliquez ici pour consulter l'enregistrement suppriméCe document a été mis à jour. Il ne s’agit pas de la dernière version publiée. Cliquez ici pour voir la dernière version du fichier.
Organisme vivant modifié
(LMO)
L’image ci-dessous identifie l’OVM par son identificateur unique, son nom commercial et un lien vers cette page du Centre d’échange Cliquer dessus pour télécharger une plus grande image sur votre ordinateur Pour du soutien sur comment utiliser, aller dans la page de liens rapides OVM.
Herculex™ I maize
EN
TC1507
Oui
DAS-Ø15Ø7-1
-
Organisation :Dow AgroSciences ()Téléphone :Télécopieur :E-mail :Site Web : http://www.dowagro.com/homepage/index.htm,
-
Organisation :Pioneer Hi-Bred International Inc. ()Secteur privé (affaires et industrie)7100 NW 62nd Avenue PO Box 1000Johnston, Iowa
50131, États-Unis d'AmériqueTéléphone : +1 515 535-3200,Télécopieur :E-mail :Site Web : www.pioneer.com/,
Maize (Zea mays) was modified for resistance to the lepidopteran European corn borer (Ostrinia nubilalis) and tolerance to herbicide glufosinate ammonium. To achieve lepidopteran resistance, the modified corn Bacillus thuringiensis expresses the Cry1F protein (delta-endotoxin/crystal protein),which has a pore forming mode of action in the epithelial lines of the feeding insect larvae. To achieve tolerance to the herbicide glufinosinate, the modified corn expresses Streptomyces viridochromogenes phosphinothricin N-acetyltransferase, which acetylates the glufosinate ammonium herbicide to form a non-toxic compound, preventing the inhibition of glutamine synthetase and thus preserving ammonia levels and nitrogen metabolism in the plant.
Le terme « organisme récepteur » désigne un organisme (non modifié ou déjà modifié) ayant fait l'objet d'une modification génétique, tandis que les « organismes parentaux » désignent les organismes impliqués dans un croisement ou une fusion cellulaire pour générer un organisme modifié.
-
BCH-ORGA-SCBD-246-6 Organisme Zea mays (Maize, Corn, MAIZE)Cultures
FR
PHI8999A derived from plasmid PHP8999
EN
- Pistolet biolistique / à particules
|
0.980 kb
|
|
1.000 kb
|
|
1.820 kb
|
|
0.720 kb
|
|
0.550 kb
|
|
0.550 kb
|
|
0.200 kb
|
Certains de ces éléments génétiques peuvent être présents sous forme de fragments ou sous forme tronquée. Veuillez consulter les notes ci-dessous, s’il y a lieu.
-
BCH-GENE-SCBD-14987-8 Cry1F | Bacillus thuringiensis (Bt, Bacillus, BACTU)Séquence codante de protéines | Résistance aux maladies et aux parasites (Insectes, Chenille tisseuse (papillons et mites))
-
BCH-GENE-SCBD-15002-5 Phosphinothricin N-acetyltransferase | Streptomyces viridochromogenes (STRVR)Séquence codante de protéines | Résistance aux herbicides (Glufosinate)
-
BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoteur
-
BCH-GENE-SCBD-100363-5 ORF25 PolyA Terminator sequence | Agrobacterium tumefaciens (Agrobacterium)Terminateur
-
BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoteur
-
BCH-GENE-SCBD-100290-6 CaMV 35S terminator | Cauliflower mosaic virus (CaMV)Terminateur
-
BCH-GENE-SCBD-103627-5 Ubiquitin Intron 1 | Zea mays (Maize, Corn, MAIZE)Intron
The DNA insert from maize DAS-Ø15Ø7-1 contained two genetic cassettes expressing the cry1F gene from Bacillus thuringiensis and the phosphinothricin N-acetyltransferase (pat) gene from Streptomyces viridochromogenes.
(1) The cry1F gene regulated by the promoter and first exon and intron of the maize ubiquitin gene. The 3' terminator sequence used was the 3' polyadenylation signal from ORF25 (Agrobacterium tumefaciens) (see footnote*). Due to the constitutive nature of the promoter, high levels of expression in all plant tissues are expected from this cassette.
(2) The pat coding sequence was under control of the Cauliflower mosaic virus 35S promoter and terminator. Due to the viral nature of the promoter, high levels of transcriptional expression in all plant tissues are expected from this genetic cassette.
(1) The cry1F gene regulated by the promoter and first exon and intron of the maize ubiquitin gene. The 3' terminator sequence used was the 3' polyadenylation signal from ORF25 (Agrobacterium tumefaciens) (see footnote*). Due to the constitutive nature of the promoter, high levels of expression in all plant tissues are expected from this cassette.
(2) The pat coding sequence was under control of the Cauliflower mosaic virus 35S promoter and terminator. Due to the viral nature of the promoter, high levels of transcriptional expression in all plant tissues are expected from this genetic cassette.
Note
- The coding sequence of both genes has been optimized to achieve a high level of expression in maize.
- The sequences of the complete cry1F and pat genes are identical to those in the original plasmid. The proteins produced in the modified plants are the ones intended, including a leucine residue (replacing a phenylalanine) at position 604 (of 605 amino acids in total) of Cry1F. This modification was introduced to create a specific restriction site for cloning purposes.
- Molecular analyses of the transformed plant show that the event TC1507 contains one site of integration of the introduced DNA which includes a full-length of the DNA fragment used for transformation (i.e. the ~6235 bp of DNA cassette containing the cry1F and pat genes) and an additional copy of the cry1F gene lacking the majority of the associated ubiquitin regulatory sequences.
- Southern analysis using a cry1F probe carried out by the European Food Safety Authority (EFSA; see document below) also revealed the presence of two cry1F inserts. The first represented the intact gene from the expression cassette. The second insert was a truncated cry1F fragment of 335bp, which is located at the 5’ end of the insertion locus. In addition, analysis of the sequences adjacent to the insert of fragment PHI8999A revealed DNA fragments that correspond to small segments from PHI8999A, including incomplete sequences from the pat coding sequence, the maize ubiquitin promoter and the terminator from Agrobacterium tumefaciens. Furthermore, different fragments of chloroplast DNA and a number of sequences with similarity to retrotransposons are also present in the border region of the insert.
Footnote *: The EFSA document indicates that the 3’ sequence from the A. tumefaciens mannopine synthase gene was used as terminator of the cry1F gene.
FR
- Biocombustible
- Nourriture pour les animaux
- Nourriture
- DAS-Ø15Ø7-1 - CropLife International Detection Methods Database [ English ]
- DAS-Ø15Ø7-1 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- DAS-Ø15Ø7-1 - CropLife International Detection Methods Database ( CropLife ) [ English ]
FR
The transformation event TC1507 is the genetic modification present in the commercial maize variety Fenaltec 22, the first locally developed modified crop released for commercial cultivation in Colombia and the first off-patent (generic) GM maize commercialized in Latin America. Developed through a collaboration between the National Federation of Cereal, Legumes, and Soybean Growers (FENALCE) and the Plant Genetic Engineering Group from National University of Colombia, it incorporates the previously approved TC1507 event into a Colombian-adapted maize variety. Following the expiration or absence of applicable patent protections, existing biotechnology events can be legally integrated into locally adapted crops, reducing development costs and dependence on proprietary technologies. Fenaltec 22 received authorization for commercial cultivation in Colombia in 2019, followed by approvals for food and feed use, marking an important milestone for public-sector agricultural biotechnology and national seed development in the region (Sánchez-Betancourt et al., 2024). See attached for publication.
- EUginius: DAS1507 [URL] [ English ]
- OECD BioTrack database: 1507 [URL] [ English ]
- ISAAA GM Approval database: TC1507 [URL] [ English ]
- FAO GM Approval database: DAS-Ø15Ø7-1 [URL] [ English ]
- BATS (2003), Genetically Modified (GM) Crops: Molecular and Regulatory details, version 2 [PDF] [ English ]
- EFSA (2005). Opinion of the Scientific Panel on Genetically Modified Organisms on placing on the market of insect-tolerant genetically modified maize 1507, for food use (EFSA-GMO-NL-2004-02) [PDF] [ English ]
- Dow AgroSciences LLC (2000): DAS-Ø15Ø7-1 [PDF] [ English ]
- FSANZ: Assessment Report A446 (corn line 1507) [PDF] [ English ]
- Canadian Food Inspection Agency (2002). DD2002-41 - Determination of the Safety of Corn (Zea mays L.) Line 1507 [URL] [ English ]
- Crop Breed. Appl. Biotechnol. (2024). First Latin American off-patent corn event - Fenaltec 22 [PDF] ( Crop Breeding and Applied Biotechnology, Brazilian Society of Plant Breeding ) [ English ]
- Crop Breed. Appl. Biotechnol. (2024). First Latin American off-patent corn event - Fenaltec 22 [PDF] ( Crop Breeding and Applied Biotechnology; Brazilian Society of Plant Breeding; Sánchez-Betancourt et al., 2024 ) [ English ]
| Type de fichier | Champs | Fichier(s) : | |
|---|---|---|---|
| Organisme vivant modifié | Organisme récepteur ou organismes parentaux | 120 | |
| Organisme vivant modifié | OVM connexe(s) | 3 | |
| Décision de pays ou tout autre communication | Identification de l'OVM | 76 | |
| Évaluation des risques générée par un processus de réglementation | Organisme(s) vivant(s) modifié(s) | 90 | |
| Laboratoire pour la détection et d'identification des OVM | OVM(s) détectable(s) par le laboratoire | 30 | |
