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Élément génétique
(GENE)
published: 09 janv. 2009
last updated: 05 mai 2016
Acetohydroxy acid synthase gene
EN
-
Acetolactate Synthase (ALS) geneEN
CS-ahas-ARATH
EN
Séquence codante de protéines
Non
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BCH-ORGA-SCBD-12098-4 Organisme Arabidopsis thaliana (Thale cress, Mouse-ear cress, Arabidopsis, ARATH)Plantes
FR
Acetohydroxy acid synthase
EN
AHAS is an essential enzyme for many organisms as it catalyzes the first step in the biosynthesis of the branched-chain amino acids valine, isoleucine, and leucine.
A common mutant form of the ahas gene was isolated from a herbicide-resistant Arabidopsis thaliana which differs from the wild type gene by only a single base pair. A "G" to "A" point mutation results in a single amino acid substitution in which the serine residue at position 653 is replaced by asparagine (S653N)
Tests using the mutant isoform of the ahas gene showed that it confers tolerance to sulfonylurea herbicides in plants. The ahas mutant isoform may, therefore, be used as a selectable marker in transgenic plants.
EN
A common mutant form of the ahas gene was isolated from a herbicide-resistant Arabidopsis thaliana which differs from the wild type gene by only a single base pair. A "G" to "A" point mutation results in a single amino acid substitution in which the serine residue at position 653 is replaced by asparagine (S653N)
Tests using the mutant isoform of the ahas gene showed that it confers tolerance to sulfonylurea herbicides in plants. The ahas mutant isoform may, therefore, be used as a selectable marker in transgenic plants.
FR
- Andersson, M. et al. 2003: A novel selection system for potato transformation using a mutated AHAS gene. Plant Cell Rep., 22: 261-267. [ English ]
- Sathasivan, K. et al. 1991: Molecular basis of imidazolinone herbicide resistance in Arabidopsis thaliana var. Colombia. Plant Physiol., 97: 1044-1050. [ English ]
- Lee, Y.-T., and Duggleby, R.G. 2000: Mutagenesis studies on the sensitivity of Escherichia coli acetohydroxyacid synthase II to herbicides and valine. Biochem. J., 350: 69-73. [ English ]
- Acetohydroxyacid Synthase.pdf [ English ]
- Herbicide resistant forms of Arabidopsis thaliana acetohydroxyacid synthase characterization of the catalytic properties and sensitivity to inhibitors of four defined mutants..pdf [ English ]
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| Organisme vivant modifié | Élément(s) génétique(s) introduit(s) ou modifié(S) | 14 | |
| Organisme vivant modifié | Construction d'éléments génétiques | 10 | |
